胱天蛋白酶1(CASP1)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Caspase 1 (CASP1)
ICE; IL1BC; IL1-BC; P45; Interleukin 1 Beta Converting Enzyme; Apoptosis-Related Cysteine Peptidase; Interleukin-1 beta convertase
- 编号SEB592Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围0.312-20ng/mL
- 灵敏度最小可检测剂量小于等于0.113ng/mL.
- 样本类型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2793 ¥ 3990 ¥ 17955 ¥ 33915 ¥ 279300
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测胱天蛋白酶1(CASP1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将胱天蛋白酶1(CASP1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的胱天蛋白酶1(CASP1)与连接于固相载体上的抗体结合，然后加入生物素化的胱天蛋白酶1(CASP1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的胱天蛋白酶1(CASP1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB592Hu02 | 胱天蛋白酶1(CASP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB592Hu01 | 胱天蛋白酶1(CASP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB592Hu03 | 胱天蛋白酶1(CASP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB592Hu04 | 胱天蛋白酶1(CASP1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB592Hu01 | 胱天蛋白酶1(CASP1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB592Hu04 | 胱天蛋白酶1(CASP1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAB592Hu02 | 胱天蛋白酶1(CASP1)多克隆抗体 | WB,IHC,ICC/IF |
| LAB592Hu71 | 胱天蛋白酶1(CASP1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| LAB592Hu81 | 胱天蛋白酶1(CASP1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| MAB592Hu22 | 胱天蛋白酶1(CASP1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEB592Hu | 胱天蛋白酶1(CASP1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB592Hu | 胱天蛋白酶1(CASP1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSB592Hu01 | 胱天蛋白酶1(CASP1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
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| Journal of Virology | NS1 protein of 2009 pandemic influenza A virus inhibits porcine NLRP3 inflammasome-mediated interleukin-1 beta production by suppressing ASC ubiquitination [Pubmed:29386291] |
| Journal of Translational Medicine | Recurrent pregnancy loss is associated to leaky gut: a novel pathogenic model of endometrium inflammation? [Pubmed:29665864] |
| Reproductive Sciences | A role for the inflammasome in spontaneous labor at term [Pubmed:27852921] |
| Journal of Virology | CHARACTERIZATION OF PORCINE NLRP3 INFLAMMASOME-MEDIATED INTERLEUKIN-1 BETA PRODUCTION IN RESPONSE TO INFLUENZA A VIRUSES [Pubmed: 29386291] |
| ACTA Otorhinolaryngologica Italica | NLRP3 inflammasome expression in lipopolysaccharide-induced otitis media [Pubmed: 30676848] |
| American Journal of Reproductive Immunology | Effect of alpha lipoic acid and myoinositol on endometrial inflammasome from recurrent pregnancy loss women [Pubmed: 31148259] |
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| ACTIVITY OF APOPTOSIS MARKERS IN HPV-ASSOCIATED CERVICAL PATHOLOGIES [] | |
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| LIFE SCIENCES | The NLRP3 inhibitor dapansutrile attenuates folic acid induced nephrotoxicity via inhibiting inflammasome/caspase-1/IL axis and regulating autophagy/proliferation [34560082] |
| Biol Reprod | The alarmin S100A12 causes sterile inflammation of the human chorioamniotic membranes and preterm birth and neonatal mortality in mice [34632484] |
| J Mol Neurosci | TRPV1 Suppressed NLRP3 Through Regulating Autophagy in Microglia After Ischemia-Reperfusion Injury [Pubmed:35041191] |
| Viruses | Influenza A Virus Infection Activates NLRP3 Inflammasome through Trans-Golgi Network Dispersion [Pubmed:35062292] |
