Ⅱ型胶原交联羧基端肽(CTXII)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Cross Linked C-Telopeptide Of Type II Collagen (CTXII)

CTX-II; CTX2

  • Ⅱ型胶原交联羧基端肽(CTXII)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • Ⅱ型胶原交联羧基端肽(CTXII)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • Ⅱ型胶原交联羧基端肽(CTXII)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA686Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测Ⅱ型胶原交联羧基端肽(CTXII),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的Ⅱ型胶原交联羧基端肽(CTXII)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-94 82
EDTA plasma(n=5) 98-105 101
heparin plasma(n=5) 89-96 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的Ⅱ型胶原交联羧基端肽(CTXII),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中Ⅱ型胶原交联羧基端肽(CTXII)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 89-105% 78-94% 93-101%
EDTA plasma(n=5) 93-101% 79-99% 82-98% 78-97%
heparin plasma(n=5) 79-90% 94-101% 95-102% 78-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将Ⅱ型胶原交联羧基端肽(CTXII)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
CPA686Hu21 Ⅱ型胶原交联羧基端肽(CTXII)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
SPA686Hu01 Ⅱ型胶原交联羧基端肽(CTXII)合成多肽 Immunogen; Blocking Peptide.
PAA686Hu01 Ⅱ型胶原交联羧基端肽(CTXII)多克隆抗体 WB; IHC; ICC; IP.
LAA686Hu71 Ⅱ型胶原交联羧基端肽(CTXII)多克隆抗体(生物素标记) WB; IHC; ICC.
CEA686Hu Ⅱ型胶原交联羧基端肽(CTXII)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA686Hu Ⅱ型胶原交联羧基端肽(CTXII)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA686Hu11 Ⅱ型胶原交联羧基端肽(CTXII)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Aging Cell Transthyretin deposition promotes progression of osteoarthritis [pubmed:28941045]
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Advances in Clinical and Experimental Medicine Fibulin-3 and other cartilage metabolism biomarkers in relationship to calprotectin (MRP8/14) and disease activity in rheumatoid arthritis patients treated with … [68362.pdf]
Journal of Orthopaedic Surgery and Research The levels of urine CTX-II, C2C, and PYD in children patients with Kashin-Beck disease in Qinghai Province of China [Pubmed: 30635000]
Nutrients The Preventive Effects of Greenshell Mussel (Perna canaliculus) on Early-Stage Metabolic Osteoarthritis in Rats with Diet-Induced Obesity [Pubmed: 31311115]
International journal of molecular sciences Differences in Osteoimmunological Biomarkers Predictive of Psoriatic Arthritis among a Large Italian Cohort of Psoriatic Patients [Pubmed: 31717649]
Applied Physiology Nutrition and Metabolism Effectiveness of collagen supplementation on pain scores in healthy individuals with self-reported knee pain; A randomized controlled trial [Pubmed: 31990581]
heliyon Oral intake of Streptococcus thermophilus improves knee osteoarthritis degeneration: A randomized, double-blind, placebo-controlled clinical study []
EBioMedicine Repopulation of decellularised articular cartilage by laser-based matrix engraving [33483297]
Biomolecules Concerted Actions by PIICP, CTXII, and TNF-¦Á in Patients with Juvenile Idiopathic Arthritis [33924892]
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