基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Matrix Gla Protein (MGP)

MGLAP; GIG36; Cell growth-inhibiting gene 36 protein

  • 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB477Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测基质Gla蛋白(MGP),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的基质Gla蛋白(MGP)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 80-97 82
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 87-98 92

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的基质Gla蛋白(MGP),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中基质Gla蛋白(MGP)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 93-101% 96-105% 96-105% 94-101%
EDTA plasma(n=5) 85-101% 79-92% 78-105% 93-103%
heparin plasma(n=5) 83-102% 94-104% 88-95% 87-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将基质Gla蛋白(MGP)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的基质Gla蛋白(MGP)与连接于固相载体上的抗体结合,然后加入生物素化的基质Gla蛋白(MGP)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质Gla蛋白(MGP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB477Hu01 基质Gla蛋白(MGP)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB477Hu01 基质Gla蛋白(MGP)多克隆抗体 WB; IHC; ICC; IP.
MAB477Hu22 基质Gla蛋白(MGP)单克隆抗体 WB; IHC; ICC; IP.
SEB477Hu 基质Gla蛋白(MGP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB477Hu 基质Gla蛋白(MGP)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
PSB477Hu01 基质Gla蛋白(MGP)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB477Hu01 基质Gla蛋白(MGP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Nephrology Dialysis Transplantation Magnesium prevents phosphate-induced calcification in human aortic vascular smooth muscle cells [PubMed: PMC3611891]
Maturitas. Bone metabolism regulators and arterial stiffness in postmenopausal women [Pubmed: 23916080]
The Egyptian Rheumatologist Osteoprotegerin (OPG) and Matrix Gla protein (MGP) in rheumatoid arthritis patients: Relation to disease activity [ScienceDirect: S1110116414000076]
Toxicology Reports Canola and hydrogenated soybean oils accelerate ectopic bone formation induced by implantation of bone morphogenetic protein in mice [Researchgate:Source]
Lipids in Health and Disease Markers of increased cardiovascular risk in patients with chronic kidney disease [Biomedcentral:Source]
Human &amp; Veterinary Medicine The behavior of circulating matrix Gla protein, matrix metalloproteinase-9 and nitrotyrosine in patients with varicose veins [Hvm:Source]
Pol Arch Med Wewn Effect of Vitamin K2 on progression of atherosclerosis and vascular calcification in nondialyzed patients with chronic kidney disease stages 3-5 [PubMed: 26176325]
Lipids Health Dis. Markers of increased atherosclerotic risk in patients with chronic kidney disease: a preliminary study [pmc:PMC4739105]
Kidney Blood Press Res. Plasma Desphospho-Uncarboxylated Matrix Gla Protein as a Marker of Kidney Damage and Cardiovascular Risk in Advanced Stage of Chronic Kidney Disease [Pubmed:27100101]
Archives of Oral Biology Matrix-Gla Protein rs4236 [A/G] gene polymorphism and serum and GCF levels of MGP in patients with subgingival dental calculus [pubmed:27348051]
Cell Death & Disease Mesenchymal stromal cells-derived matrix Gla protein contribute to the alleviation of experimental colitis [Pubmed:29880866]
Urology High concentration of calcium promotes mineralization in NRK52E cells via inhibiting the expression of matrix Gla protein [Pubmed:29935264]
Clinica Chimica Acta Serum total matrix Gla protein: Reference interval in healthy adults and variations in patients with vascular and osteoarticular diseases [Pubmed: 30597137]
Biological Trace Element Research Altered Mineral Metabolism and Disequilibrium Between Calcification Promoters and Inhibitors in Chronic Hemodialysis Patients [Pubmed: 30847765]
Renal Failure Relationship of matrix Gla protein and vitamin K with vascular calcification in hemodialysis patients [Pubmed: 31538831]
Heliyon Matrix Gla protein maintains normal and malignant hematopoietic progenitor cells by interacting with bone morphogenetic protein-4 [Pubmed: 32322728]
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