骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Periostin (POSTN)

OSF-2; PDLPOSTN; PN; Osteoblast Specific Factor 2

  • 骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEH339Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测骨膜蛋白(POSTN),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的骨膜蛋白(POSTN)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 80-89 85
heparin plasma(n=5) 81-101 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的骨膜蛋白(POSTN),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中骨膜蛋白(POSTN)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-104% 98-105% 98-105% 93-104%
EDTA plasma(n=5) 79-93% 98-105% 97-105% 86-101%
heparin plasma(n=5) 94-102% 87-98% 88-96% 79-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将骨膜蛋白(POSTN)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的骨膜蛋白(POSTN)与连接于固相载体上的抗体结合,然后加入生物素化的骨膜蛋白(POSTN)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的骨膜蛋白(POSTN)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPH339Hu03 骨膜蛋白(POSTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPH339Hu01 骨膜蛋白(POSTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPH339Hu02 骨膜蛋白(POSTN)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAH339Hu01 骨膜蛋白(POSTN)多克隆抗体 WB; IHC; ICC; IP.
PAH339Hu02 骨膜蛋白(POSTN)多克隆抗体 WB; IHC; ICC; IP.
PAH339Hu03 骨膜蛋白(POSTN)多克隆抗体 WB; IHC; ICC; IP.
LAH339Hu71 骨膜蛋白(POSTN)多克隆抗体(生物素标记) WB; IHC; ICC.
MAH339Hu23 骨膜蛋白(POSTN)单克隆抗体 WB; IHC; ICC; IP.
MAH339Hu24 骨膜蛋白(POSTN)单克隆抗体 WB
MAH339Hu21 骨膜蛋白(POSTN)单克隆抗体 WB
MAH339Hu27 骨膜蛋白(POSTN)单克隆抗体 WB; IHC; ICC; IP.
MAH339Hu29 骨膜蛋白(POSTN)单克隆抗体 WB; IHC; ICC; IP.
MAH339Hu22 骨膜蛋白(POSTN)单克隆抗体 WB; ICC/IF
SEH339Hu 骨膜蛋白(POSTN)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMH339Hu 骨膜蛋白(POSTN)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Blood Circulating Periostin Is Elevated In Patients With Hemoglobinopathies and Correlates With Bone Mineral Density In Double Heterozygous Sickle-Cell/Beta-Thalassemia Patients; A Novel Marker Of Bone Strength? [Bloodjournal: Source]
The Journal of Clinical Endocrinology & Metabolism Serum periostin is associated with fracture risk in postmenopausal women: a 7 years prospective analysis of the OFELY study [Pubmed: 24628551]
J Clin Endocrinol Metab. Serum Periostin Is Associated With Fracture Risk in Postmenopausal Women: A 7-Year Prospective Analysis of the OFELY Study [Pubmed:24628551]
Rheumatology (Oxford). Circulating periostin levels in patients with AS: association with clinical and radiographic variables, inflammatory markers and molecules involved in bone formation [Pubmed:25349442]
Journal of Periodontal Research Assessment of periostin levels in serum and gingival crevicular fluid of patients with periodontal disease [Pubmed:25529858]
新医学 血清内脂素水平与多囊卵巢综合征的相关性研究 [Xinyixue:Source]
Osteoarthritis Serum periostin is associated with prevalent knee osteoarthritis and disease incidence/progression in women: the OFELY study [PubMed: 26072384]
Endocrine Serum periostin is a potential biomarker for non-alcoholic fatty liver disease: a case– control study [PubMed: 26362060]
Blood Cancer Journal High levels of periostin correlate with increased fracture rate, diffuse MRI pattern, abnormal bone remodeling and advanced disease stage in patients with newly … [pubmed:27716740]
Metabolism.  Low periostin levels in adult patients with Langerhans cell histiocytosis are independently associated with the disease activity. [pubmed:28521873]
Osteoporosis International Circulating periostin levels increase in association with bone density loss and healing progression during the early phase of hip fracture in Chinese older women [pubmed:28382553]
Molecular Diagnosis & Therapy The Utility of Biomarkers in Osteoporosis Management [pubmed:28271451]
Journal of Bone and Mineral Research Cortical and trabecular bone microstructure did not recover at weight-bearing skeletal sites and progressively deteriorated at non-weight-bearing sites during the year following International Space Station missions [doi:10.1002]
Clinical Cases in Mineral and Bone Metabolism Periostin and sclerostin levels in juvenile Paget's disease [pubmed:29263750]
Calcified Tissue International Development of a New Immunoassay for Human Cathepsin K-Generated Periostin Fragments as a Serum Biomarker for Cortical Bone [pubmed:28725907]
Wiley Characterization of a sandwich ELISA for the quantification of all human periostin isoforms [pubmed:28493527]
International Osteoporosis Foundation and National Osteoporosis Foundation Interaction between LRP5 and periostin gene polymorphisms on serum periostin levels and cortical bone microstructure [pubmed:29038835]
Bone Effect of age and gender on serum periostin: Relationship to cortical measures, bone turnover and hormones [pubmed:28323143]
Clinica Chimica Acta Association between serum periostin concentrations and outcome after acute spontaneous intracerebral hemorrhage [pubmed:28882488]
Clinica Chimica Acta Serum periostin concentrations and outcomes after severe traumatic brain injury [pubmed:28668564]
Tumor Biology Predictive and prognostic value of serum periostin in advanced non–small cell lung cancer patients receiving chemotherapy [pubmed:28459197]
Journal of Bone and Mineral Research Serum Levels of a Cathepsin-K Generated Periostin Fragment Predict Incident Low-Trauma Fractures in Postmenopausal Women Independently of BMD and FRAX [pubmed:28766739]
ОСОБЕННОСТИ СОСТОЯНИЯ ЗДОРОВЬЯ НОВОРОЖДЕННЫХ, РОЖДЕННЫХ ОТ МАТЕРЕЙ С БРОНХИАЛЬНОЙ АСТМОЙ [2017; 62:(4)]
Endocrine Circulating periostin in patients with nonalcoholic fatty liver disease. [pubmed:27738886]
Molecular Medicine Reports Periostin cross‑reacts with the renin‑angiotensin system during liver fibrosis development [pubmed:28849131]
The Journal of Biological Chemistry Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]
Calcified Tissue International The C-Terminal Intact Forms of Periostin (iPTN) Are Surrogate Markers for Osteolytic Lesions in Experimental Breast Cancer Bone Metastasis [Pubmed:29916127]
Journal of Endocrinological Investigation Increased serum periostin concentrations are associated with the presence of diabetic retinopathy in patients with type 2 diabetes mellitus [Pubmed:29349642]
Wiener klinische Wochenschrift Rheumatoid arthritis in remission []
Clinical and Experimental Medicine Myostatin and other musculoskeletal markers in lung transplant recipients [Pubmed: 30317402]
Experimental and Clinical Endocrinology & Diabetes Polycystic Ovary Syndrome is Associated with Elevated Periostin Levels [Pubmed: 30372763]
ИСПОЛЬЗОВАНИЕ СЫВОРОТОЧНОГО ПЕРИОСТИНА В КАЧЕСТВЕ МАРКЕРА ОБОСТРЕНИЙ АСТМЫ У ДЕТЕЙ []
Bone Tartrate-resistant acid phosphatase 5b, but not periostin, is useful for assessing Paget's disease of bone [Pubmed: 31051316]
Journal of Clinical Laboratory Analysis Identification and diagnostic value of pleural fluid periostin and serum periostin of malignant pleural effusions in patients with non–small‐cell lung cancer [Pubmed: 31268191]
Bone Serum periostin is associated with cancer mortality but not cancer risk in older home-dwelling men: A 8-year prospective analysis of the STRAMBO study [Pubmed: 31812700]
FASEB J Fibronectin rescues aberrant phenotype of endothelial cells lacking either CCM1, CCM2 or CCM3 [Pubmed: 32515053]
Journal of Reproductive Immunology Serum and placental periostin levels in women with early pregnancy loss [Pubmed: 32460058]
Annals of Medical Research Evaluation of periostin levels in gingival crevicular fluid and peri-implant sulcus fluid in patients with periodontal and peri-implanter disease: A cross-sectional … []
Myostatin and Markers of Bone Metabolism in Dermatomyositis []
Am J Physiol Heart Circ Physiol Periostin promotes arterial calcification through PPAR¦Ã-related glucose metabolism reprogramming [33834866]
International Journal of Chronic Obstructive Pulmonary Disease High Blood Eosinophil and YKL-40 Levels, as Well as Low CXCL9 Levels, are Associated with Increased Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease [33814903]
J Bone Oncol Serum total periostin is an independent marker of overall survival in bone metastases of lung adenocarcinoma [34150488]
Rambam Maimonides Med J The Relationship between Serum Periostin Levels and Neutrophil¨CLymphocyte Ratio with Lesion Localization and Acute Prognosis in Acute Ischemic Stroke [34137680]
Rambam Maimonides Medical Journal First Admission Neutrophil–Lymphocyte Ratio May Indicate Acute Prognosis of Ischemic Stroke [34137680]
留言咨询