凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Prothrombin Fragment 1+2 (F1+2)
- 编号SEA710Hu
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围3.9-250pg/mL
- 灵敏度最小可检测剂量小于等于1.5pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测凝血酶原片段F1+2(F1+2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的凝血酶原片段F1+2(F1+2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 78-99 | 93 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的凝血酶原片段F1+2(F1+2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中凝血酶原片段F1+2(F1+2)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-99% | 78-103% | 85-102% | 98-105% |
| EDTA plasma(n=5) | 88-101% | 83-104% | 84-98% | 84-101% |
| heparin plasma(n=5) | 80-99% | 84-99% | 84-101% | 88-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将F1抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的F1与连接于固相载体上的抗体结合，然后加入生物素化的F2抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的F1+2呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA710Hu01 | 凝血酶原片段F1+2(F1+2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA710Hu01 | 凝血酶原片段F1+2(F1+2)多克隆抗体 | WB; IHC; ICC; IP. |
| LAA710Hu71 | 凝血酶原片段F1+2(F1+2)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAA710Hu22 | 凝血酶原片段F1+2(F1+2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA710Hu23 | 凝血酶原片段F1+2(F1+2)单克隆抗体 | WB; IHC; ICC/IF |
| MAA710Hu24 | 凝血酶原片段F1+2(F1+2)单克隆抗体 | WB; IHC |
| MAA710Hu25 | 凝血酶原片段F1+2(F1+2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA710Hu21 | 凝血酶原片段F1+2(F1+2)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA710Hu26 | 凝血酶原片段F1+2(F1+2)单克隆抗体 | WB; IHC; ICC; IP. |
| FAA710Hu02 | 抗凝血酶原片段F1+2(F1+2)单克隆抗体 | Flow cytometry. |
| LAA710Hu72 | 凝血酶原片段F1+2(F1+2)单克隆抗体(生物素标记) | WB; IHC; ICC. |
| SEA710Hu | 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA710Hu | 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA710Hu | 凝血酶原片段F1+2(F1+2)检测试剂盒(化学发光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA710Hu | 凝血酶原片段F1+2(F1+2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSA710Hu01 | 凝血酶原片段F1+2(F1+2)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSA710Hu01 | 凝血酶原片段F1+2(F1+2)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
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| Journal of Vascular Surgery | Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair [ScienceDirect: S0741521411018568] |
| Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine | High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study [Sjtrem:1757-7241] |
| Journal of Thrombosis and Haemostasis | High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality [Wiley: source] |
| British Journal of Dermatology | Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria [PubMed: 21910697] |
| Arteriosclerosis, Thrombosis, and Vascular Biology. | Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status [PubMed: 22859493] |
| Journal of Vascular Surgery | Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [PubMed: 23140799] |
| PLoS ONE | Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension [Plosone: Source] |
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| Cogent Medicine | Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction [Tandfonline:Source] |
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| J Thromb Haemost | Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism [PubMed: 26514101] |
| Acta Oncologica Turcica | Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer [aot:AOT_49_1_6_12.pdf] |
| Advances in Medical Sciences | Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation [pubmed:29145170] |
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| Acta Haematologica | Elevations of Thrombotic Biomarkers in Hemoglobin H Disease [Pubmed:29402840] |
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| Rheumatol Int | The impact of anti-endothelial cell antibodies (AECAs) on the development of blood vessel damage in patients with systemic lupus erythematosus: the preliminary … [Pubmed:35284968] |
