凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Prothrombin Fragment 1+2 (F1+2)

  • 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA710Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测凝血酶原片段F1+2(F1+2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的凝血酶原片段F1+2(F1+2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
sodium citrate plasma(n=5) 96-104 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的凝血酶原片段F1+2(F1+2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中凝血酶原片段F1+2(F1+2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 92-101% 86-103% 79-90% 97-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将F1抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的F1与连接于固相载体上的抗体结合,然后加入生物素化的F2抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的F1+2呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA710Hu01 凝血酶原片段F1+2(F1+2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA710Hu01 凝血酶原片段F1+2(F1+2)多克隆抗体 WB; IHC; ICC; IP.
LAA710Hu71 凝血酶原片段F1+2(F1+2)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA710Hu21 凝血酶原片段F1+2(F1+2)单克隆抗体 WB; IHC; ICC; IP.
LAA710Hu72 凝血酶原片段F1+2(F1+2)单克隆抗体(生物素标记) WB; IHC; ICC.
SEA710Hu 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA710Hu 凝血酶原片段F1+2(F1+2)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA710Hu 凝血酶原片段F1+2(F1+2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
PSA710Hu01 凝血酶原片段F1+2(F1+2)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA710Hu01 凝血酶原片段F1+2(F1+2)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

参考文献

杂志 参考文献
Critical Care Disseminated intravascular coagulation or acute coagulopathy of trauma shock early after trauma? An observational study [BioMed: cc10553]
Journal of Vascular Surgery Changes in thrombin generation, fibrinolysis, platelet and endothelial cell activity, and inflammation following endovascular abdominal aortic aneurysm repair [ScienceDirect: S0741521411018568]
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine High levels of soluble VEGF receptor 1 early after trauma are associated with shock, sympathoadrenal activation, glycocalyx degradation and inflammation in severely injured patients: a prospective study [Sjtrem:1757-7241]
Journal of Thrombosis and Haemostasis High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality [Wiley: source]
British Journal of Dermatology Evaluation of autologous plasma skin test in patients with chronic idiopathic urticaria [PubMed: 21910697]
Arteriosclerosis, Thrombosis, and Vascular Biology. Diet Modulates Endogenous Thrombin Generation, A Biological Estimate of Thrombosis Risk, Independently of the Metabolic Status [PubMed: 22859493]
Journal of Vascular Surgery Medium-term effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [PubMed: 23140799]
PLoS ONE Markers of Thrombogenesis and Fibrinolysis and Their Relation to Inflammation and Endothelial Activation in Patients with Idiopathic Pulmonary Arterial Hypertension [Plosone: Source]
Journal of Vascular Surgery Effect of endovascular and open abdominal aortic aneurysm repair on thrombin generation and fibrinolysis [Pubmed: 23140799 ]
Haemetology Clinical impact of factor V Leiden, prothrombin G20210A, and MTHFR C677T mutations among sickle cell disease patients of Central India [Pubmed: 23992124]
Cogent Medicine Study the association of adiponectin with inflammation and hypercoagulability in case of type 2 diabetic subjects with renal dysfunction [Tandfonline:Source]
Interact Cardiovasc Thorac Surg. An ex vivo evaluation of blood coagulation and thromboresistance of two extracorporeal circuit coatings with reduced and full heparin dose [Pubmed:24632424]
Perfusion Ex vivo simulation of cardiopulmonary bypass with human blood for hemocompatibility testing [PubMed: 26243277]
J Thromb Haemost Comparison of the Effect of Dabigatran and Dalteparin on Thrombus Stability in a Murine Model of Venous Thromboembolism [PubMed: 26514101]
Acta Oncologica Turcica Thrombin activatable fibrinolysis inhibitor (TAFI), tissue factor pathway inhibitor (TFPI), and prothrombin fragment 1+2 levels in patients with advanced colorectal cancer [aot:AOT_49_1_6_12.pdf]
Advances in Medical Sciences Perioperative thrombocytopenia predicts poor outcome in patients undergoing transcatheter aortic valve implantation [pubmed:29145170]
Thrombosis Research The causes of thrombocytopenia after transcatheter aortic valve implantation [pubmed:28582640]
Acta Haematologica Elevations of Thrombotic Biomarkers in Hemoglobin H Disease [Pubmed:29402840]
Journal of Neurotrauma The cerebral thrombin system is activated after intracerebral hemorrhage and contributes to secondary lesion growth and poor neurological outcome in C57Bl/6 mice [Pubmed: 31830857]
Indian Journal of Public Health Research & Development Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever []
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