谷丙转氨酶(ALT)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Alanine Aminotransferase (ALT)
GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
- 编号SEA207Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围1.56-100ng/mL
- 灵敏度最小可检测剂量小于等于0.61ng/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测谷丙转氨酶(ALT)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的谷丙转氨酶(ALT)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 93-102 | 96 |
| EDTA plasma(n=5) | 92-105 | 97 |
| heparin plasma(n=5) | 92-105 | 99 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的谷丙转氨酶(ALT)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中谷丙转氨酶(ALT)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-95% | 88-102% | 87-95% | 82-99% |
| EDTA plasma(n=5) | 92-105% | 90-97% | 82-94% | 80-104% |
| heparin plasma(n=5) | 79-94% | 87-101% | 79-102% | 96-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将谷丙转氨酶(ALT)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的谷丙转氨酶(ALT)与连接于固相载体上的抗体结合，然后加入生物素化的谷丙转氨酶(ALT)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的谷丙转氨酶(ALT)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA207Mu01 | 谷丙转氨酶(ALT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA207Mu02 | 谷丙转氨酶(ALT)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA207Mu02 | 谷丙转氨酶(ALT)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA207Mu01 | 谷丙转氨酶(ALT)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA207Mu | 谷丙转氨酶(ALT)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA207Mu | 谷丙转氨酶(ALT)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA207Mu01 | 谷丙转氨酶(ALT)检测试剂盒DIY材料(酶联免疫吸附试验法) | 自己动手制作ELISA试剂盒的主要材料 |
参考文献
| 杂志 | 参考文献 |
| Cancer Gene Therapy | Enhanced antitumor activity by combining an adenovirus harboring ING4 with cisplatin for hepatocarcinoma cells [Nature: 201067] |
| Endocrinology. | Additive effects of nicotine and high-fat diet on hepatic steatosis in male mice. [PubMed: 23093702] |
| Kidney International | Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms [PubMed: PMC3612362] |
| Proceedings of the National Academy of Sciences | Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model [PubMed: PMC3382536] |
| African Journal of Pharmacy and Pharmacology | Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats [Academicjournals: Source] |
| Kidney International | Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms [Pubmed: 23325074] |
| European Journal of Pharmacology | Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats [ScienceDirect: S0014299914000235] |
| Eur J Nutr. | Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis [Pubmed:25657013] |
| Experimental and Molecular Pathology | Resolvin E1 and chemerin C15 peptide do not improve rodent non-alcoholic steatohepatitis [Pubmed:25681756] |
| Gastroenterol Res Pract | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061] |
| FNG & Bilim Tıp Dergisi | The effects of subacute deltamethrin exposure on rat liver: histochemical, immunohistochemical, and biochemical study [xmlui:11446] |
| Gastroenterol Res Pract. | Advanced Studies in Clinical and Experimental Research in Gastroenterology [Pubmed:26955389] |
| DARU Journal of Pharmaceutical Sciences | Effects of developmental exposure to silver in ionic and nanoparticle form: A study in rats [pubmed:27716437] |
| Oncotarget | Dihydromyricetin protects against liver ischemia/reperfusion induced apoptosis via activation ofFOXO3a-mediated autophagy. [pubmed:27793014] |
| Scientific Reports | Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. [pubmed:27678302] |
| Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485] |
| Gastroenterology Research and Practice | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model [pubmed:28912805] |
| PLoS Pathogens | Alcohol-associated intestinal dysbiosis impairs pulmonary host defense against Klebsiella pneumoniae. [pubmed:28604843] |
| FEBS Open Bio | A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis [Pubmed:29321963] |
| Rolle von CMKLR1 und dessen Liganden bei der nicht-alkoholischen Fettlebererkrankung [Regensburg:Source] | |
| Cumhuriyet Medical Journal | The valuable effects of potent antioxidant curcumin in cisplatin induced liver and kidney injury [DOI:Source] |
| BMC Complementary and Alternative Medicine | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910] |
| Scientific Reports | Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cycle [Pubmed: 30787318] |
| Brain Behavior and Immunity | Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| Frontiers in Immunology | CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid Metabolism [Pubmed: 32158445] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313] |
| European Journal of Biology | Effects of Tempol in Lipopolysaccharide-Induced Liver Injury [] |
| Pathogens | Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus [33925018] |
| Gastroenterol Res Pract | An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment [34804155] |
| Int Immunopharmacol | Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes [34848154] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver … [Pubmed:35212074] |
| Medcomm | Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and … [Pubmed:35548710] |
| International Immunopharmacology | Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation [Pubmed:35605433] |
