神经胶质纤维酸性蛋白(GFAP)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)
Intermediate Filament Protein
- 编号SEA068Mu
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于6.0pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2722 ¥ 3888 ¥ 17496 ¥ 33048 ¥ 272160
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测神经胶质纤维酸性蛋白(GFAP)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的神经胶质纤维酸性蛋白(GFAP)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 80-102 | 83 |
| EDTA plasma(n=5) | 87-96 | 92 |
| heparin plasma(n=5) | 85-98 | 88 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的神经胶质纤维酸性蛋白(GFAP)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中神经胶质纤维酸性蛋白(GFAP)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 88-101% | 91-98% | 78-101% | 78-92% |
| EDTA plasma(n=5) | 88-101% | 94-102% | 78-94% | 92-105% |
| heparin plasma(n=5) | 99-105% | 88-105% | 96-105% | 96-104% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将神经胶质纤维酸性蛋白(GFAP)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的神经胶质纤维酸性蛋白(GFAP)与连接于固相载体上的抗体结合，然后加入生物素化的神经胶质纤维酸性蛋白(GFAP)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的神经胶质纤维酸性蛋白(GFAP)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA068Mu03 | 神经胶质纤维酸性蛋白(GFAP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Mu02 | 神经胶质纤维酸性蛋白(GFAP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Mu01 | 神经胶质纤维酸性蛋白(GFAP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA068Mu04 | 神经胶质纤维酸性蛋白(GFAP)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA068Mu03 | 神经胶质纤维酸性蛋白(GFAP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA068Mu02 | 神经胶质纤维酸性蛋白(GFAP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA068Mu01 | 神经胶质纤维酸性蛋白(GFAP)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA068Mu04 | 神经胶质纤维酸性蛋白(GFAP)多克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu22 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu23 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu21 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu24 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu25 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu27 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu28 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA068Mu29 | 神经胶质纤维酸性蛋白(GFAP)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA068Mu | 神经胶质纤维酸性蛋白(GFAP)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA068Mu | 神经胶质纤维酸性蛋白(GFAP)检测试剂盒(酶联免疫吸附试验法,小样本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA068Mu | 神经胶质纤维酸性蛋白(GFAP)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSA068Mu01 | 神经胶质纤维酸性蛋白(GFAP)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Occupational Health | Effects of Exposure to 1-Bromopropane on Astrocytes and Oligodendrocytes in Rat Brain [Pubmed: 23183024] |
| Morfovirtual 2014 | EFECTO PROTECTOR DEL TRATAMIENTO CON NEUROEPO SOBRE LA GLIA DE LA CORTEZA CEREBRAL EN RATONES TRANSGéNICOS CON LA ENFERMEDAD DE ALZHEIMER. [Sld:Source] |
| Annals of Anatomy | Effect of memantine, a NMDA receptor blocker, on ethambutol-induced retinal injury [PubMed: 26704355] |
| Mediators of Inflammation | IL-4 Inhibits IL-1β-Induced Depressive-Like Behavior and Central Neurotransmitter Alterations [PubMed: 26417153] |
| Neurochem Res | Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436] |
| Cell Mol Neurobiol | Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [PubMed: 26119304] |
| Sleep and Biological Rhythms | Relationship of orexin (hypocretin) system and astrocyte activation in Parkinson's disease with hypersomnolence [Doi: 10.1111] |
| Cellular and Molecular Neurobiology | Hypericum Perforatum Hydroalcoholic Extract Mitigates Motor Dysfunction and is Neuroprotective in Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson’s Disease [Pubmed:26119304] |
| Ann Anat | Effect of memantine: A NMDA receptor blocker, on ethambutol-induced retinal injury [Pubmed:26704355] |
| United States Patent Application | ESTROGENIC COMPONENTS FOR USE IN THE TREATMENT OF NEUROLOGICAL DISORDERS [y2016:0101116.html] |
| Clinical Research | Human plasma glial fibrillary acidic protein and heat shock protein 27 concentrations in acute moderate-intensity aerobic exercise with different duration [a825e1e27b0221df9a627769c0f614432e92.pdf] |
| Medical Journal of Indonesia | Comparison of GFAP and HSP27 concentrations in acute moderate-intensity aerobic exercise of different duration [2031884] |
| Peptides. | Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064] |
| european journal of pharmacology | Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041] |
| european journal of pharmacology | Troxerutin exerts neuroprotection in 6-hydroxydopamine lesion rat model of Parkinson's disease: Possible involvement of PI3K/ERβ signaling. [pubmed:28284752] |
| biomedicine & pharmacotherapy | Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883] |
| J Endocrinol. | Estrogen receptors and estetrol-dependent neuroprotective actions: a pilot study. [pubmed:27799463] |
| IOS Press Content Library | An Intranasal Formulation of Erythropoietin (Neuro-EPO) Prevents Memory Deficits and Amyloid Toxicity in the APPSwe Transgenic Mouse Model of Alzheimer's … [articles:journal-of-alzheimers-disease] |
| Tierärztliche Hochschule Hannover | Evaluierung von Biomarkern im Liquor cerebrospinalis bei paraplegischen Hunden mit Rückenmarksverletzung [:] |
| Nigerian Journal of Clinical Practice | Utilization of Glial Fibrillary Acidic Protein and Galectin-3 in the Diagnosis of Cerebral Infarction Patients with Normal Cranial Tomography [pubmed:28406123] |
| Cytokine | Soy isoflavone genistein attenuates lipopolysaccharide-induced cognitive impairments in the rat via exerting anti-oxidative and anti-inflammatory effects [pubmed:29102164] |
| Biomedicine & pharmacotherapy | Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403] |
| Experimental gerontology | The anti-aging protein klotho alleviates injury of nigrostriatal dopaminergic pathway in 6-hydroxydopamine rat model of Parkinson's disease: Involvement of PKA/CaMKII/CREB signaling. [pubmed:29107062] |
| Pesticide Biochemistry and Physiology | Acrolein-mediated neurotoxicity in growing Wistar male rats [Pubmed:30033014] |
| Journal of Perinatal Medicine | Mechanisms of death in structurally normal stillbirths [Pubmed: 30231013] |
| Metabolic Brain Disease | Berberine ameliorates lipopolysaccharide-induced learning and memory deficit in the rat: insights into underlying molecular mechanisms [Pubmed: 30456649] |
| Metabolic Brain Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms [Pubmed: 30421246] |
| 博士论文 | INSTITUTO DE CIENCIAS BÁSICAS Y PRECLÍNICAS “VICTORIA DE GIRÓN” DEPARTAMENTO DE HISTOLOGÍA [] |
| Medicina | The Administration of the New Pyrimidine Derivative—4-{2-[2-(3, 4-Dimethoxyphenyl)-Vinyl]-6-Ethyl-4-Oxo-5-Phenyl-4H-Pyrimidine-1-Il} Benzsulfamide Restores the … [] |
| Open Access Macedonian Journal of Medical Sciences | Inhibition of Receptor for Advanced Glycation End Products as New Promising Strategy Treatment in Diabetic Retinopathy [Pubmed: 32165929] |
| NEUROPSYCHOBIOLOGY | Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752] |
| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326] |
| Acta Histochemica | Quercetin mitigates monosodium glutamate-induced excitotoxicity of the spinal cord motoneurons in aged rats via p38 MAPK inhibition [Pubmed: 32622428] |
| stanbul Med J | The Predictive Role of Neurobiochemical Markers in Multiple Sclerosis. [] |
| Basic and Clinical Neuroscience | Paeonol Protects Against Intrastriatal 6-Hydroxydopamine Rat Model of Parkinson's Disease [33995926] |
| pharmacology online | PHARMACOLOGICAL SCREENING OF A POTENTIALLY EFFECTIVE COMPOUND FOR THE TREATMENT OF CHRONIC TRAUMATIC ENCEPHALOPATHY?¡ [] |
| Neuropeptides | The bee venom active compound melittin protects against bicuculline-induced seizures and hippocampal astrocyte activation in rats [34808488] |
| Neural Plast | P2Y2 Receptor Mediated Neuronal Regeneration and Angiogenesis to Affect Functional Recovery in Rats with Spinal Cord Injury [Pubmed:35154311] |
留言咨询
