基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Matrix Metalloproteinase 2 (MMP2)

CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase

  • 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA100Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测基质金属蛋白酶2(MMP2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的基质金属蛋白酶2(MMP2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-103 83
heparin plasma(n=5) 90-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的基质金属蛋白酶2(MMP2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中基质金属蛋白酶2(MMP2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 83-99% 84-98% 83-101% 96-103%
heparin plasma(n=5) 89-97% 85-101% 97-105% 87-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将基质金属蛋白酶2(MMP2)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的基质金属蛋白酶2(MMP2)与连接于固相载体上的抗体结合,然后加入生物素化的基质金属蛋白酶2(MMP2)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的基质金属蛋白酶2(MMP2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA100Mu01 基质金属蛋白酶2(MMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA100Mu01 基质金属蛋白酶2(MMP2)活性蛋白 Cell culture; Activity Assays.
RPA100Mu02 基质金属蛋白酶2(MMP2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA100Mu01 基质金属蛋白酶2(MMP2)多克隆抗体 WB; IHC; ICC; IP.
PAA100Mu02 基质金属蛋白酶2(MMP2)多克隆抗体 WB; IHC; ICC; IP.
SEA100Mu 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA100Mu 基质金属蛋白酶2(MMP2)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA100Mu 基质金属蛋白酶2(MMP2)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA100Mu 基质金属蛋白酶2(MMP2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Moleculer Vision Bone morphogenetic protein-2: a potential regulator in scleral remodeling [PubMed: PMC2605409]
Neuroimmunomodulation Upregulated Expression of Matrix Metalloproteinases and Tissue Inhibitors of Matrix Metalloproteinases in BALB/c Mouse Brain Challenged with Japanese Encephalitis Virus [Pubmed: source]
Zahedan Journal of Research in Medical Sciences The Effect of Acute Sub-Maximal Endurance Exercise on Serum Angiogenic Indices in Sedentary Men [Zjrms: Source]
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source]
Experimental & Translational Stroke Medicine Early outcome and blood-brain barrier integrity after co-administered thrombolysis and hyperbaric oxygenation in experimental stroke [PubMed: 21679435]
PLoS One Downregulation of CD9 in Keratinocyte Contributes to Cell Migration via Upregulation of Matrix Metalloproteinase-9 [PubMed: PMC3797697]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Clinical and Investigative Medicine The protective effect of dexmedetomidine on bupivacaine-induced sciatic nerve inflammation is mediated by mast cells [Pubmed: 23544611]
Physiol. Res. The unwounded skin remodeling in animal models of diabetes types 1 and 2. [Pubmed: 24020818]
Acta Histochemica RNA interference-mediated knockdown of Notch-1 inhibits migration and invasion, down-regulates matrix metalloproteinases and suppresses NF-κB signaling pathway in trophoblast cells [Pubmed: 24681113]
Peritoneal Dialysis Can effluent matrix metalloproteinase 2 and plasminogen activator inhibitor 1 be used as biomarkers of peritoneal membrane alterations in peritoneal dialysis patients? [Pubmed: 23994841]
Polski Przeglad Chirurgiczny Concentration of gelatinases and their tissue inhibitors in pancreatic inflammatory and neoplastic tumors and their influence on the early postoperative course [Pubmed: 23585206]
Thromb Haemost. Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655]
Int Immunopharmacol.? Cadherin-11 involves in synovitis and increases the migratory and invasive capacity of fibroblast-like synoviocytes of osteoarthritis [Pubmed:25824718]
Journal of Perinatology Increased expression of prostasin contributes to early-onset severe preeclampsia through inhibiting trophoblast invasion [Pubmed:25078863]
Biomedical Materials In vivo bioengineered ovarian tumors based on collagen, matrigel, alginate and agarose hydrogels: a comparative study [Pubmed:25634132]
Stem Cells International Matrix Metalloproteinase 9 Secreted by Hypoxia Cardiac Fibroblasts Triggers Cardiac Stem Cell Migration In Vitro [Pubmed:25767513]
Physiology and Pharmacology A Study on the Serum Levels of Angiogenic Factors in Response to Acute Long-term Submaximal Exercise in Sedentary Men [Phypha:Source]
Mediators of Inflammation Metalloproteinases and Their Tissue Inhibitors in Comparison between Different Chronic Pneumopathies in the Horse [Journals: Mi]
Journal of Biomaterials and Tissue Engineering MicroRNA-506 Up-Regulation Inhibits Growth and Invasion in Non-Small Cell Lung Cancer Cells by Possibly Targeting Sphingosine Kinase 1 [Content: Asp]
Biomedical Materials In vivo [PubMed: 25634132]
Int J Clin Exp Pathol Up-regulation of microRNA-302a inhibited the proliferation and invasion of colorectal cancer cells by regulation of the MAPK and PI3K/Akt signaling pathways [PubMed: 26191138]
Hattori and Ishihara Stem Cell Research & Therapy Altered protein secretions during interactions between adipose tissue-or bone marrow-derived stromal cells and inflammatory cells [PubMed: 25884474]
Biosens Bioelectron Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V3+ [PubMed: 26433066]
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140]
Canadian Journal of Physiology and Pharmacology Resveratrol reduces matrix metalloproteinases and alleviates intrahepatic cholestasis of pregnancy in rats [Doi: Abs]
PLASTIC AND AESTHETIC RESEARCH A comparative study to evaluate the effect of limited access dressing on diabetic ulcers [Article: 2347-9264]
Int J Mol Sci Evodiamine Attenuates PDGF-BB-Induced Migration of Rat Vascular Smooth Muscle Cells through Activating PPARγ [PubMed: 26703570]
Basic Research CTGF upreCavia (Guinea pig )lation correlates with MMP-9 level in airway remodeling in a murine model of asthma [Journal:27699]
International Journal of Oncology 1, 25-Dihydroxyvitamin D3 alleviates salivary adenoid cystic carcinoma progression by suppressing GPX1 expression through the NF-κB pathway [Pubmed:26782341]
International Journal of Pharmacology Research Article Effects of DNMT and HDAC Inhibitors (RG108 and Trichostatin A) on NGF-induced Neurite Outgrowth and Cellular Migration [profile:57162df608aebf0697f1843d]
Journal of Stroke and Cerebrovascular Diseases Epigallocatechin Gallate Extends the Therapeutic Window of Recombinant Tissue Plasminogen Activator Treatment in Ischemic Rats [Pubmed:26851971]
VirusDisease Circulating levels of matrix metalloproteinases and tissue inhibitors of matrix metalloproteinases during Japanese encephalitis virus infection [Pubmed:26925446]
Cell Physiol Biochem. MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1 [Pubmed:27160777]
Int Wound J. From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases. [Pubmed:26991748]
Biosensors and Bioelectronics Ultrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+ conjugates [science:S0956566315304474]
world journal of surgery Metalloproteinases and Their Inhibitors in Patients with Inguinal Hernia. [pubmed:28050662]
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. [pubmed:28348451]
Balkan Medical Journal Anti-inflammatory and Anti-apoptotic Effect of Valproic Acid and Doxycycline Independent from MMP Inhibition in Early Radiation Damage [pubmed:27761275]
Microvascular Research Pleiotropic effects of survivin in vascular endothelial cells. [pubmed:27373518]
BMC Veterinary Research Metalloproteinases and their inhibitors are influenced by inhalative glucocorticoid therapy incombination with environmental dust reduction in equine recurrent airway obstruction. [pubmed:27938355]
Cellular Physiology and Biochemistry MicroRNA-340 Induces Apoptosis and Inhibits Metastasis of Ovarian Cancer Cells by Inactivation of NF-x03BA;B1. [pubmed:27160777]
Journal of Biomaterials and Tissue Engineering MicroRNA-340 Targets NF-κB1 to Inhibit Cell Proliferation, Migration and Invasion in Hepatocellular Carcinoma Cell Line [publication:306067745]
World Journal of Plastic Surgery Superficial Burn Wound Healing with Intermittent Negative Pressure Wound Therapy UnderLimited Access and Conventional Dressings. [pubmed:27853690]
Journal of Biomaterials and Tissue Engineering Zoledronic Acid Inhibits TGF-β1-Induced the Proliferation, Migration and EMT Through Smad Pathway in Human Gingival Fibroblasts [art00008]
Human cell Liver X receptor α (LXRα) promoted invasion and EMT of gastric cancer cells by regulation of NF-κB activity [pubmed:28091828]
Human Cell MicroRNA-124 upregulation inhibits proliferation and invasion of osteosarcoma cells by targeting sphingosine kinase 1 [pubmed:27743351]
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422]
Human Cell MicroRNA-30a functions as tumor suppressor and inhibits the proliferation and invasion of prostate cancer cells by down-regulation of SIX1 [pubmed:28573504]
Cellular Physiology and Biochemistry The MiR-495/Annexin A3/P53 Axis Inhibits the Invasion and EMT of Colorectal Cancer Cells [pubmed:29224019]
International Wound Journal From varices to venous ulceration: the story of chronic venous disease described by metalloproteinases [pubmed:26991748]
Fundamental & Clinical Pharmacology Epigallocatechin-3-gallate ameliorates intrahepatic cholestasis of pregnancy by inhibiting matrix metalloproteinase-2 and matrix metalloproteinase-9 [DOI:10.1111/fcp.12294]
PLoS One Pro-fibrotic compounds induce stellate cell activation, ECM-remodelling and Nrf2 activation in a human 3D-multicellular model of liver fibrosis [pubmed:28665955]
Nanotoxicology Cellular proliferation and differentiation induced by single-layer molybdenum disulfide and mediation mechanisms of proteins via the Akt-mTOR-p70S6K signaling pathway [10.1080/17435390.2017.1357213]
Archives of Medical Science CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma. [pubmed:28507585]
Molecular Medicine Reports Lysyl oxidase is involved in synovial hyperplasia and angiogenesis in rats with collagen‑induced arthritis [pubmed:28901438]
European Journal of Medicinal Chemistry Syntenin-targeted peptide blocker inhibits progression of cancer cells [Pubmed:29857221]
Journal of Neurochemistry Liraglutide ameliorated peripheral neuropathy in diabetic rats: Involvement of oxidative stress, inflammation and extracellular matrix remodeling [Pubmed:29572844]
Journal of Cellular Biochemistry Matrix metalloproteinases‐2 (MMP‐2) and matrix metalloproteinases‐9 (MMP‐9) are differentially expressed in different regions of normal and preeclampsia … [10.1002:jcb.26849]
Inflammation Extracellular Matrix Remodeling and Modulation of Inflammation and Oxidative Stress by Sulforaphane in Experimental Diabetic Peripheral Neuropathy [Pubmed:29704151]
OncoTargets and Therapy sirT4 acts as a tumor suppressor in gastric cancer by inhibiting cell proliferation, migration, and invasion [Pubmed:30022839]
Biomed Research International CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling [Pubmed:29984229]
APMIS Hepatoprotectivity of Panduratin A against liver damage: demonstration with a rat model of cirrhosis induced by thioacetamide [Pubmed:30058214]
Molecular Medicine Reports Effect of a synthetic inhibitor of urokinase plasminogen activator on the migration and invasion of human cervical cancer cells in vitro [Pubmed:29328476]
Oncology Letters miRNA‑199a‑5p suppresses proliferation and invasion by directly targeting NF‑κB1 in human ovarian cancer cells [10.3892:ol.2018.9170]
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663]
Open Medicine MMP-2 and TIMP-2 in patients with heart failure and chronic kidney disease [Pubmed:29915813]
Pediatric Research Anti-thymic stromal lymphopoietin antibody suppresses airway remodeling in asthma through reduction of MMP and CTGF. [Pubmed: 30464333]
Advanced Healthcare Materials Impact of Hydrogel Elasticity and Adherence on Osteosarcoma Cells and Osteoblasts [Pubmed: 30838809]
Cellular & Molecular Biology Letters MiR-613 inhibits proliferation and invasion and induces apoptosis of rheumatoid arthritis synovial fibroblasts by direct down-regulation of DKK1 []
Mediators of Inflammation Metalloproteinases and their Inhibitors under the Course of Immunostimulation by CPG-ODN and Specific Antigen Inhalation in Equine Asthma []
Journal of endovascular therapy: an official journal of the International Society of Endovascular Specialists Correlation of Baseline Plasma and Inguinal Connective Tissue Metalloproteinases and Their Inhibitors With Late High-Pressure Endoleak After Endovascular … [Pubmed: 31464166]
Oncology Research MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 [Pubmed: 31796150]
Scientific Reports Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722]
Acta Biomaterialia Stiffness of the Aligned Fibers Affects Structural and Functional Integrity of the Oriented Endothelial Cells [Pubmed: 32205213]
JOURNAL OF THROMBOSIS AND THROMBOLYSIS Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289]
JOURNAL OF ETHNOPHARMACOLOGY Danlou tablet inhibits the inflammatory reaction of high-fat diet-induced atherosclerosis in ApoE knockout mice with myocardial ischemia via the NF-κB signaling … [Pubmed: 32745509]
heliyon Matrix levels of metalloproteinase-2 (MMP-2) and toxicity evaluation of carbonate apatite-based endodontic sealer in rat subcutaneous implantation [Pubmed: 32671254]
J Cell Physiol A novel role of VEPH1 in regulating AoSMC phenotypic switching [Pubmed: 32342520]
Advances in Animal and Veterinary Sciences Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats []
Alteration on MMP-2 Levels in Women Diabetes Patients in Basrah []
Veterinary Sciences: Research and Reviews Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep []
MEDIATORS OF INFLAMMATION MMP-9 Concentration in Peritoneal Fluid Is a Valuable Biomarker Associated with Endotoxemia in Equine Colic [33488296]
Oncol Lett GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression [33613720]
Journal of Pregnancy Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia [33381317]
Immunopharmacol Immunotoxicol Tofacitinib enhances remyelination and improves myelin integrity in cuprizone-induced mice [34618622]
Exp Cell Res Netrin-like domain of sFRP4, a Wnt antagonist inhibits stemness, metastatic and invasive properties by specifically blocking MMP-2 in cancer stem cells from human … [34762897]
Sci Rep Curcumin derivative ST09 modulates the miR-199a-5p/DDR1 axis and regulates proliferation and migration in ovarian cancer cells [34837026]
Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer [34650982]
Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980]
Journal of Pharmaceutical Research International Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid … []
Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … []
Turkish Journal of Biochemistry-Turk Biyokimya Dergisi Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes []
留言咨询