去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Noradrenaline (NE)

NA; NAd; Norepinephrine; Norepi

  • 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • CEA907Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测去甲肾上腺素(NE),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的去甲肾上腺素(NE)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 85-102 95
EDTA plasma(n=5) 97-105 101
heparin plasma(n=5) 99-105 102

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的去甲肾上腺素(NE),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中去甲肾上腺素(NE)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 88-96% 95-104% 78-89%
EDTA plasma(n=5) 87-101% 89-104% 81-102% 96-103%
heparin plasma(n=5) 96-104% 88-96% 80-98% 99-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)50µL,
    加入50µL检测液A(临用前配制);
    37°C温育1小时。
3. 洗板3次;
4. 加检测溶液B100µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物90µL,37°C孵育10-20分钟;
7. 加终止液50µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将去甲肾上腺素(NE)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA907Ge11 去甲肾上腺素(NE)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA907Ge21 去甲肾上腺素(NE)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA907Ge01 去甲肾上腺素(NE)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA907Ge81 去甲肾上腺素(NE)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA907Ge25 去甲肾上腺素(NE)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA907Ge24 去甲肾上腺素(NE)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA907Ge23 去甲肾上腺素(NE)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA907Ge21 去甲肾上腺素(NE)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA907Ge 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA907Ge 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
HEA907Ge 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA907Ge 去甲肾上腺素(NE)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA907Ge 去甲肾上腺素(NE)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA907Ge11 去甲肾上腺素(NE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Renal Failure Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients [Pubmed: 23530612]
Acta Physiologica Apelin-13 and APJ in paraventricular nucleus contribute to hypertension via sympathetic activation and vasopressin release in spontaneously hypertensive rats [Pubmed:24995933]
Physiological Research Renal Sympathetic Denervation Improves Cardiac Dysfunction [PubMed: 25804096]
J Cardiovasc Pharmacol Ther Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of 伪1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta. [PubMed: 26025460]
Neuropsychiatr Dis Treat Catha edulis chewing effects on treatment of paranoid schizophrenic patients [PubMed: 25926735]
Journal of Caediovascular Pharmacology and Therapeutics Erythropoietin Reverses Sepsis-Induced Vasoplegia to Norepinephrine Through Preservation of α1D-Adrenoceptor mRNA Expression and Inhibition of GRK2-Mediated Desensitization in Mouse Aorta [content:21]
Neuropharmacology Relaxin in paraventricular nucleus contributes to sympathetic overdrive and hypertension via PI3K-Akt pathway [Pubmed:26746861]
Microchemical Journal Determination of 17β-estradiol and noradrenaline in dog serum using surface-enhanced Raman spectroscopy and random Forest [science:S0026265X16300388]
Scientific Reports Reduced lipolysis response to adipose afferent reflex involved in impaired activation of adrenoceptor-cAMP-PKA-hormone sensitive lipase pathway in obesity [pubmed:27694818]
PLoS One. Artepillin C, a Typical Brazilian Propolis-Derived Component, Induces Brown-Like Adipocyte Formation in C3H10T1/2 Cells, Primary Inguinal White Adipose Tissue-Derived Adipocytes, and Mice. [pubmed:27598888]
Cellular Physiology and Biochemistry Renal Denervation Attenuates Multi-Organ Fibrosis and Improves Vascular Remodeling in Rats with Transverse Aortic Constriction Induced Cardiomyopathy [pubmed:27889753]
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. [pubmed:28070608]
Acta Physiologica c-Jun N-terminal Kinase mediates prostaglandin-induced sympathoexcitation in rats with chronic heart failure by reducing GAD1 and GABRA1 expression [pubmed:27439062]
Nutrition Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats [S0899900716302350]
Auris Nasus Larynx. Stress hormonal changes in the brain and plasma after acute noise exposure in mice. [pubmed:27496010]
Oncotarget Effects of renal denervation on monocrotaline induced pulmonary remodeling [pubmed:28187460]
JOURNAL OF APPLIED PHYSIOLOGY  Renal sympathetic denervation attenuates hypertension and vascular remodeling in renovascular hypertensive rats [pubmed:27742806]
Nutrition Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats [pubmed:28241996]
Acta Physiologica Beneficial Effects of Renal Denervation on Cardiac Angiogenesis in Rats with Prolonged Pressure Overload [pubmed:27575955]
Proceedings of the National Academy of Sciences of the United States of America (PNAS) cAMP-inducible coactivator CRTC3 attenuates brown adipose tissue thermogenesis [Pubmed:29784793]
Toxicology Reports Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor … [Pubmed:29854627]
International Journal of Nursing Practice Non‐pharmacological interventions during childbirth for pain relief, anxiety, and neuroendocrine stress parameters: A randomized controlled trial [Pubmed:29512230]
Acta Astronautica A novel sympathetic neuronal GABA ergic signaling system regulates NE release to prevent ventricular arrhythmias after acute myocardial infarction []
Journal of Taibah University for Science Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles []
Journal of the American College of Cardiology Noninvasive Stereotactic Radiotherapy for Renal Denervation in a Swine Model [Pubmed: 31558254]
American Journal of Physiology-Heart and Circulatory Physiology Exacerbated pressor and sympatho-excitatory effects of central Elabela in spontaneously hypertensive rats [Pubmed: 31834836]
European Journal of Pharmacology Resiniferatoxin reduces ventricular arrhythmias in heart failure via selectively blunting cardiac sympathetic afferent projection into spinal cord in rats [Pubmed: 31811858]
A porcine model for studying the cardiovascular consequences of high‐thoracic spinal cord injury [Pubmed: 31876952]
The American Journal of Pathology Sympathetic Nerves Positively Regulate Eosinophil-Driven Allergic Conjunctivitis via α1-Adrenergic Receptor Signaling [Pubmed: 32194050]
pharmaceutical biology Role of melatonin in intestinal mucosal injury induced by restraint stress in mice [Pubmed: 32298156]
Nature Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714]
European Journal of Medical Research Laparoscopic-based perivascular renal sympathetic nerve denervation: a feasibility study in a porcine model [Pubmed: 32552871]
Scientific Reports Baroreceptor denervation reduces inflammatory status but worsens cardiovascular collapse during systemic inflammation [Pubmed: 32332859]
bioRxiv Baroreceptor denervation reduces inflammatory status and worsens cardiovascular collapse during systemic inflammation []
Blockade of adrenergic β‐receptor activation through local delivery of propranolol from a 3D collagen/polyvinyl alcohol/hydroxyapatite scaffold promotes bone repair … []
Cogent Medicine Fractionation and bioassay-guided isolation of antihypertensive components of senecio serratuloides []
PHARMACOLOGICAL RESEARCH Piezo2 channel in nodose ganglia neurons is essential in controlling hypertension in a pathway regulated directly by Nedd4-2 [33352230]
ACTA PHARMACOLOGICA SINICA The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990]
Mol Metab Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D [33549845]
International Journal of?Nanomedicine Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ¡­ [33762821]
Int J Hypertens The Effects of Renal Nerve Denervation on Blood Pressure and Target Organs in Different Hypertensive Rat Models [33884205]
Kidney Blood Press Res Renal interstitial fibrosis is reduced in high-fat diet-induced obese pigs following renal denervation from the intima and adventitia of the renal artery [34852339]
Hypertension Expression of LHCGR (Luteinizing Hormone/Chorionic Gonadotrophin Receptor) in Pheochromocytomas Unveils an Endocrine Mechanism Connecting Pregnancy … [Pubmed:35189708]
Exp Eye Res Autonomic nervous system receptor-mediated regulation of mast cell degranulation modulates the inflammation after corneal epithelial abrasion [Pubmed:35421396]
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