载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Apolipoprotein A1 (APOA1)

Apo-A1; ApoA-1 Milano; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I

  • 载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA519Po.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测载脂蛋白A1(APOA1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的载脂蛋白A1(APOA1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 83-93 87
EDTA plasma(n=5) 82-98 91
heparin plasma(n=5) 94-102 98

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的载脂蛋白A1(APOA1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中载脂蛋白A1(APOA1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 84-96% 89-101% 89-97% 86-101%
EDTA plasma(n=5) 88-102% 82-103% 81-95% 80-102%
heparin plasma(n=5) 91-105% 91-98% 83-97% 88-95%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将载脂蛋白A1(APOA1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的载脂蛋白A1(APOA1)与连接于固相载体上的抗体结合,然后加入生物素化的载脂蛋白A1(APOA1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的载脂蛋白A1(APOA1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Sus scrofa; Porcine (Pig,猪) 应用(仅供研究使用,不用于临床诊断!)
RPA519Po01 载脂蛋白A1(APOA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA519Po02 载脂蛋白A1(APOA1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA519Po01 载脂蛋白A1(APOA1)多克隆抗体 WB; IHC; ICC; IP.
PAA519Po02 载脂蛋白A1(APOA1)多克隆抗体 WB; IHC; ICC; IP.
LAA519Po81 载脂蛋白A1(APOA1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA519Po71 载脂蛋白A1(APOA1)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA519Po24 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Po23 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Po22 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
MAA519Po21 载脂蛋白A1(APOA1)单克隆抗体 WB; IHC; ICC; IP.
SEA519Po 载脂蛋白A1(APOA1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA519Po 载脂蛋白A1(APOA1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
European Journal of Applied Physiology Endurance training enhances ABCA1 expression in rat small intestine [PubMed: 19629515]
Official Journal of the INWR Effect of eight weeks of wrestling and circuit fitness training on Apo lipoportein A-I and lymphocyte ABCA1 gene expression in well-trained wrestler. [Wrestling: source]
Biochemical and Biophysical Research Communications Treadmill exercise enhances ABCA1 expression in rat liver [ScienceDirect: S0006291X0701488X]
World Journal of Sport Sciences The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Training on Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein A-1 [Idosi: source]
Biochimica et Biophysica Acta Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ]
Biomarkers The association of a distinct plasma proteomic profile with the cervical high-grade squamous intraepithelial lesion of Uyghur women: a 2D liquid-phase chromatography/mass spectrometry study [PubMed: 22458349]
Journal of the American Heart Association MicroRNA-33 Deficiency Reduces the Progression of Atherosclerotic Plaque in ApoE?/? Mice [PubMed: PMC3540673]
PLoS ONE Apolipoprotein AI Is a Potential Mediator of Remote Ischemic Preconditioning [Plosone: Source]
Molecular Genetics and Metabolism DYRK1A overexpression decreases plasma lecithin: cholesterol acyltransferase activity and apolipoprotein AI levels [Pubmed: 23920041]
Endocrinology Deficiency of Clusterin Exacerbates High-Fat Diet-Induced Insulin Resistance in Male Mice [Pubmed: 24684302]
Molecular Genetics and Metabolism Reports Corrective effects of hepatotoxicity by hepatic Dyrk1a gene delivery in mice with intermediate hyperhomocysteinemia [Sciencedirect:S2214426915000026]
J Psychopharmacol Anti-atherogenic properties of high-density lipoproteins in psychiatric patients before and after two months of atypical anti-psychotic therapy [PubMed: 26253619]
Biochim Biophys Acta Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253]
Pharmacol Res Perspect Efficacy of tomato concentrates in mouse models of dyslipidemia and cancer [PubMed: 26171234]
Clin Exp Nephrol Differentially expressed urinary biomarkers in children with idiopathic nephrotic syndrome [PubMed: 26351173]
Oncogene Apolipoprotein A-I inhibits experimental colitis and colitis-propelled carcinogenesis [PubMed: 26279300]
Oncogene Apolipoprotein AI inhibits experimental colitis and colitis-propelled carcinogenesis [Pubmed:26279300]
Allergy Serum levels of 9α,11β-PGF2 and apolipoprotein A1 achieve high predictive power as biomarkers of anaphylaxis. [pubmed:28378321]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
11 High-Density Lipoproteins Exert Pro-inflammatory Effects on Macrophages via Passive Cholesterol Depletion and PKC-NF-kB/STAT1-IRF1 Signaling [pubmed:27866837]
Biomedical Science Letters Effect of Ginkgo biloba Extract (EGb 761) on Serum Cholesterol Levels in Wild-type C57Bl/6 Mice [10.15616/BSL.2017.23.2.80]
ACS Biomaterials Science & Engineering Enhanced Antiatherosclerotic Efficacy of Statin-Loaded Reconstituted High-Density Lipoprotein via Ganglioside GM1 Modification [abs:10.1021]
Food Research International Wild Lonicera caerulea berry polyphenol extract reduces cholesterol accumulation and enhances antioxidant capacity in vitro and in vivo [Pubmed:29580541]
Journal of The Science of Food and Agriculture Consumption of orange fermented beverage improves antioxidant status and reduces peroxidation lipid and inflammatory markers in healthy humans [Pubmed:29124773]
Toxicon A membrane disrupting toxin from wasp venom underlies the molecular mechanism of tissue damage [Pubmed:29654869]
European Journal of Pharmacology The effect of exenatide (a GLP-1 analog) and sitagliptin (a DPP-4 inhibitor) on plasma platelet-activating factor acetylhydrolase (PAF-AH) activity and concentration in?… [Pubmed: 30768981]
European Journal of Preventive Cardiology High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats [Pubmed: 31718266]
Diagnostics Systemic Alterations of Immune Response-Related Proteins during Glaucoma Development in the Murine Model DBA/2J [Pubmed: 32585848]
VETERINARY PATHOLOGY Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234]
Nat Commun GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313]
Cardiovasc Toxicol Interplay of Obesity, Ethanol, and Contaminant Mixture on Clinical Profiles of Cardiovascular and Metabolic Diseases: Evidence from an Animal Study [Pubmed:35429258]
Peptides Neuropeptide Y promotes hepatic apolipoprotein A1 synthesis and secretion through neuropeptide Y Y5 receptor [Pubmed:35660638]
Journal of Functional Foods Castanea mollissima shell polyphenols regulate JAK2 and PPARγ expression to suppress inflammation and lipid accumulation by inhibiting M1 macrophages … []
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