碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

  • 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA551Rb.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测碱性成纤维细胞生长因子(FGF2),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的碱性成纤维细胞生长因子(FGF2)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 88-101 94
EDTA plasma(n=5) 86-97 90
heparin plasma(n=5) 92-102 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的碱性成纤维细胞生长因子(FGF2),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中碱性成纤维细胞生长因子(FGF2)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 84-97% 85-93% 86-101% 82-99%
EDTA plasma(n=5) 79-101% 90-97% 93-102% 85-97%
heparin plasma(n=5) 85-98% 91-101% 97-105% 89-96%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
TMB底物 1×9mL 终止液 1×6mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)

实验原理

将碱性成纤维细胞生长因子(FGF2)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的碱性成纤维细胞生长因子(FGF2)与连接于固相载体上的抗体结合,然后加入生物素化的碱性成纤维细胞生长因子(FGF2)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的碱性成纤维细胞生长因子(FGF2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPA551Rb02 碱性成纤维细胞生长因子(FGF2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA551Rb01 碱性成纤维细胞生长因子(FGF2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA551Rb51 碱性成纤维细胞生长因子(FGF2)多克隆抗体 WB; IHC; ICC; IP.
PAA551Rb52 碱性成纤维细胞生长因子(FGF2)多克隆抗体 WB; IHC; ICC; IP.
SEA551Rb 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

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