碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- 编号SEA551Rb
- 物种Oryctolagus cuniculus (Rabbit，兔) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3h
- 检测范围15.6-1,000pg/mL
- 灵敏度最小可检测剂量小于等于5.9pg/mL.
- 样本类型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
-
产品包装(模拟)
-
产品包装(模拟)
-
实验结果图
-
标准曲线图
-
通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测碱性成纤维细胞生长因子(FGF2)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的碱性成纤维细胞生长因子(FGF2)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 88-101 | 94 |
| EDTA plasma(n=5) | 86-97 | 90 |
| heparin plasma(n=5) | 92-102 | 95 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的碱性成纤维细胞生长因子(FGF2)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中碱性成纤维细胞生长因子(FGF2)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 84-97% | 85-93% | 86-101% | 82-99% |
| EDTA plasma(n=5) | 79-101% | 90-97% | 93-102% | 85-97% |
| heparin plasma(n=5) | 85-98% | 91-101% | 97-105% | 89-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。
实验原理
将碱性成纤维细胞生长因子(FGF2)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的碱性成纤维细胞生长因子(FGF2)与连接于固相载体上的抗体结合，然后加入生物素化的碱性成纤维细胞生长因子(FGF2)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的碱性成纤维细胞生长因子(FGF2)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。
增值服务
相关产品
| 编号 | 适用物种：Oryctolagus cuniculus (Rabbit，兔) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA551Rb02 | 碱性成纤维细胞生长因子(FGF2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA551Rb01 | 碱性成纤维细胞生长因子(FGF2)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA551Rb51 | 碱性成纤维细胞生长因子(FGF2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA551Rb52 | 碱性成纤维细胞生长因子(FGF2)多克隆抗体 | WB; IHC; ICC; IP. |
| PAA551Rb01 | 碱性成纤维细胞生长因子(FGF2)多克隆抗体 | WB; IHC; ICC; IP. |
| SEA551Rb | 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA551Rb | 碱性成纤维细胞生长因子(FGF2)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940] |
| International Journal of Nanomedicine | Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis [PubMed: PMC3254262] |
| Archives of Animal Nutrition | Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis [Tandfonline: 644918] |
| Diabetes | Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia [Pubmed: 22266669] |
| Molecular Pharmacology | Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats [Pubmed: 22091745] |
| Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source] |
| Nanoscale Research Letters | Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. [PubMed: 22827927] |
| PLoS One | Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis [PubMed: PMC3771850] |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| Archives of Animal Nutrition | Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis [Pubmed: 23952606] |
| Journal of Rehabilitation Research & Development | Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness wounds [Pubmed: 23934870] |
| PLoS One.? | Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity [Pubmed:25426952] |
| Implant Dentistry | Effects of Hemostatic Agents on Fibroblast Cells [Lww:Source] |
| Journal of Materials Chemistry B | bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing [Rsc:Source] |
| Archives of Animal Nutrition | Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos [Pubmed:25530495] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972] |
| Theriogenology | Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article] |
| Tumor Biology | Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Acta Biomaterialia | Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa [article:S1742706115301410] |
| 22 | Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars [Pubmed:26774564] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| BMC Neuroscience | 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA [pubmed:27456338] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [898221] |
| Biomedicine & Pharmacotherapy | FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis [pubmed:28841454] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [pubmed:28052053] |
| International Journal of Clinical and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| Journal of Biomaterials and Tissue Engineering | The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice [10.1166/jbt.2017.1652] |
| Stem Cells and Development | Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration [Pubmed:29630447] |
| Neuroscience Letters | Increased serum FGF2 levels in first-episode, drug-free patients with schizophrenia [Pubmed: 30172685] |
| Biochemistry and Cell Biology | Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells [Pubmed: 30359541] |
| Journal of Materials Chemistry B | Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and … [] |
| BMC Ophthalmology | HOXA9 is a novel myopia risk gene [Pubmed: 30674274] |
| Applied Materials Today | Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration [] |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
| Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells [] |
| Chemico-Biological Interactions | Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor [Pubmed: 31669319] |
| International Journal of Molecular Sciences | β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe Disease [Pubmed: 31905906] |
| Molecular Medicine Reports | Long non‑coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR‑590‑3p under hypoxic conditions [Pubmed: 32323787] |
| Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells [] | |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| Materials Science & Engineering C | Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing [33812576] |
| Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨¹zerine etkisi [] | |
| World J Hepatol | Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis [Pubmed:35070009] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
留言咨询
