肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Hepatocyte Growth Factor (HGF)

HGF; F-TCF; HGFB; HPTA; SF; Scatter Factor; Hepapoietin A; Fibroblast-Derived Tumor Cytotoxic Factor; Lung Fibroblast-Derived Mitogen

  • 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA047Rb.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测肝细胞生长因子(HGF),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的肝细胞生长因子(HGF)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 92-101 97
EDTA plasma(n=5) 92-99 96
heparin plasma(n=5) 78-96 89

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的肝细胞生长因子(HGF),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中肝细胞生长因子(HGF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 98-105% 99-105% 82-103% 96-103%
EDTA plasma(n=5) 85-105% 85-95% 81-94% 98-105%
heparin plasma(n=5) 90-101% 78-99% 81-97% 89-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将肝细胞生长因子(HGF)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的肝细胞生长因子(HGF)与连接于固相载体上的抗体结合,然后加入生物素化的肝细胞生长因子(HGF)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的肝细胞生长因子(HGF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPA047Rb01 肝细胞生长因子(HGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA047Rb51 肝细胞生长因子(HGF)多克隆抗体 WB; IHC; ICC; IP.
MAA047Rb21 肝细胞生长因子(HGF)单克隆抗体 WB; IHC; ICC; IP.
SEA047Rb 肝细胞生长因子(HGF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA047Rb 肝细胞生长因子(HGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.

参考文献

杂志 参考文献
Gene Therapy and Molecular Biology Sphingosine kinase-1 protects transplanted mesenchymal stem cells and improves the performance of the infarcted heart [LWW: 01000]
Journal of Translational Medicine Low temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinoma [BioMed: 14795876]
Digestive Diseases and Sciences Gene Transfer of c-met Confers Protection Against d-Galactosamine/Lipopolysaccharide-Induced Acute Liver Failure [SpringerLink: k22823l138x2344x]
Biomarker insights Correlation Between Hepatocyte Growth Factor (HGF) and Gamma-Aminobutyric Acid (GABA) Plasma Levels in Autistic Children [PubMed: PMC3694825]
Biomarker insights Decreased Hepatocyte Growth Factor (HGF) and Gamma Aminobutyric Acid (GABA) in Individuals with Obsessive-Compulsive Disorder (OCD) [PubMed: PMC3762604]
BMC cell biology BMSCs reduce rat granulosa cell apoptosis induced by cisplatin and perimenopause [PubMed: PMC3640998]
Cell Journal (Yakhteh) Effect of Mesenchymal Stem Cells on Doxorubicin-Induced Fibrosis [PubMed: PMC3584430]
Gastroenterol Res Pract Proinflammatory Cytokines (IL-1α, IL-6) and Hepatocyte Growth Factor in Patients with Alcoholic Liver Cirrhosis [PubMed: 26448742]
Tumor Biology Hypoxia-induced secretion of IL-11 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151]
Cytotherapy Cell type–dependent variation in paracrine potency determines therapeutic efficacy against neonatal hyperoxic lung injury [PubMed: 25863963]
Biomedicine & Pharmacotherapy Silymarin-loaded Eudragit® RS100 nanoparticles improved the ability of silymarin to resolve hepatic fibrosis in bile duct ligated rats [Pubmed:27261582]
Can J Physiol Pharmacol.  Piceatannol increases the expression of hepatocyte growth factor and IL-10 thereby protecting hepatocytes in thioacetamide-induced liver fibrosis [Pubmed:27186801]
Genet Mol Res Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064]
Changes in HGF levels after a progressive test before and after Tabata HIIT program [profile:Pawel_Wolanski3]
留言咨询