细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Intercellular Adhesion Molecule 1 (ICAM1)

CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor

  • 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA548Rb.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测细胞间粘附分子1(ICAM1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的细胞间粘附分子1(ICAM1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 87-94 90
heparin plasma(n=5) 96-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的细胞间粘附分子1(ICAM1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中细胞间粘附分子1(ICAM1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 79-101% 95-103% 79-97%
EDTA plasma(n=5) 81-89% 80-101% 93-105% 84-101%
heparin plasma(n=5) 78-95% 90-101% 88-96% 97-104%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将细胞间粘附分子1(ICAM1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的细胞间粘附分子1(ICAM1)与连接于固相载体上的抗体结合,然后加入生物素化的细胞间粘附分子1(ICAM1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞间粘附分子1(ICAM1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPA548Rb01 细胞间粘附分子1(ICAM1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA548Rb21 细胞间粘附分子1(ICAM1)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA548Rb51 细胞间粘附分子1(ICAM1)多克隆抗体 WB; IHC; ICC; IP.
PAA548Rb01 细胞间粘附分子1(ICAM1)多克隆抗体 WB; IHC; ICC; IP.
MAA548Rb21 细胞间粘附分子1(ICAM1)单克隆抗体 WB; IHC; ICC; IP.
SEA548Rb 细胞间粘附分子1(ICAM1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA548Rb 细胞间粘附分子1(ICAM1)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Amran et al. Lipids in Health and Disease Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. [BioMed: 1476511X]
Evidence-based Complementary and Alternative Medicine Preventive Effects of a Natural Anti-Inflammatory Agent, Astragaloside IV, on Ischemic Acute Kidney Injury in Rats [PubMed: PMC3703719]
British Journal of Medical and Health Sciences EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS [Researchgate: Source]
Cytokine Astragaloside IV ameliorates renal injury in streptozotocin-induced diabetic rats through inhibiting NF-κB-mediated inflammatory genes expression. [Pubmed: 23434274]
BioMed research international Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192]
Journal of Veterinary Internal Medicine Markers of coagulation activation, endothelial stimulation, and inflammation in dogs with babesiosis [Pubmed: 23875771]
Arch Dis Child PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns [Bmj:Source]
Arch Dis Child? PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome [Bmj:Source]
Arch Dis Child PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography [Bmj:Source]
J Thorac Dis.? Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension [Pubmed:Pmc4015009]
Journal of Immunotoxicology Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice [Pubmed:24873678]
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
Int J Neurosci. The effect of therapeutic hypothermia after cardiopulmonary resuscitation on ICAM-1 and NSE levels in sudden cardiac arrest rabbits [Pubmed:25111247]
Naunyn-Schmiedeberg&#039;s Archives of Pharmacology Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits [Pubmed:25172523]
Nutr Res. Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128]
Experimental &amp; Molecular Medicine Elevated levels of serum sICAM-1 in asphyxiated low birth weight newborns [Pubmed:25358349]
Neural Regen Res.? Inflammatory response and neuronal necrosis in rats with cerebral ischemia [Pubmed:25422636]
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880]
CNS Neuroscience &amp; Therapeutics Peroxisome Proliferator-activated Receptor-γ Agonist Pioglitazone Ameliorates White Matter Lesion and Cognitive Impairment in Hypertensive Rats [Pubmed:25611692]
Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats [Sciencedirect:S2090506814000268]
Canadian Journal of Physiology and Pharmacology Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism [Doi: Abs]
J Cell Biochem Tumor Necrosis Factor-Alpha Up-Regulates ICAM-1 Expression and Release in Intestinal Myofibroblasts by Redox-Dependent and -Independent Mechanisms [PubMed: 26177712]
Neurotoxicology Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats [PubMed: 25845300]
Alexandria Journal of Medicine Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in [Science: Article]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140]
Experimental Eye Research The effect of triamcinolone acetonide or bevacizumab on the levels of proinflammatory cytokines after retinal laser photocoaCavia (Guinea pig )lation in pigmented rabbits [Pubmed:27296072]
Chinese Chemical Letters Screening the anti-gout traditional herbs from TCM using an in vitro method [science:S1001841716301450]
Am J Physiol Lung Cell Mol Physiol Systematic phenotyping and correlation of biomarkers with lung function and histology in lung fibrosis [Pubmed:26993522]
Mol Neurobiol A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655]
Comparative Clinical Pathology Identification of some plasma biomarkers associated with early weaning stress in crossbred piglets [article:10.1007]
Journal of Cellular Biochemistry Tumor Necrosis Factor‐Alpha Up‐Regulates ICAM‐1 Expression and Release in Intestinal Myofibroblasts by Redox‐Dependent and‐Independent Mechanisms [pubmed:26177712]
BMC Vet Res. Blood markers of fibrinolysis and endothelial activation in canine babesiosis. [pubmed:28363279]
Biomedicine & pharmacotherapy= Biomedecine & pharmacotherapie Protective effects of N-acetylcystein and atorvastatin against renal and hepatic injury in a rat model of intestinal ischemia-reperfusion [pubmed:28273631]
Pakistan journal of pharmaceutical sciences Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. [pubmed:29084680]
Experimental Parasitology Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422]
Molecular Medicine Reports Age‑related differences in serum MFG‑E8, TGF‑β1 and correlation to the severity of atherosclerosis determined by ultrasound. [pubmed:29152651]
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
International Journal of Clinical and Experimental Pathology Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881]
Oncotarget Global gene expression profile of cerebral ischemia-reperfusion injury in rat MCAO model [pubmed:29088811]
Microbial Pathogenesis Protective effects of sinomenine against LPS-induced inflammation in piglets. [pubmed:28757275]
Journal of ophthalmology Panretinal Photocoagulation Using Short-Pulse Laser Induces Less Inflammation and Macular Thickening in Patients with Diabetic Retinopathy [pubmed:28761761]
The Journal of Biological Chemistry Toll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]
Life Sciences Lipoxin A4 may attenuate the progression of obesity-related glomerulopathy by inhibiting NF-κB and ERK/p38 MAPK-dependent inflammation [Pubmed:29499280]
International Journal of Molecular Medicine Aspirin modulates the inflammatory response in a thrombus‑stimulated LMVEC model [Pubmed:29568915]
Clinical And Experimental Pharmacology And Physiology  Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336]
Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010]
Bioscience Reports N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation. [Doi: 10.1042/BSR20180858]
Prostaglandins & Other Lipid Mediators Detoxification and activating blood circulation decoction reduces restenosis involving the TLR4/NF-κB pathway after balloon injury [Pubmed: 30453089]
Biochemical and Biophysical Research Communications The anti-inflammatory and anti-oxidative effects of conbercept in treatment of macular edema secondary to retinal vein occlusion [Pubmed: 30558792]
Nutrition & Metabolism L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835]
Cytokine Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls [Pubmed: 30716657]
European Journal of Pharmacology The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in … [Pubmed: 30826323]
International journal of molecular sciences Cellular Metabolomics Reveal the Mechanism Underlying the Anti-Atherosclerotic Effects of Aspirin Eugenol Ester on Vascular Endothelial Dysfunction [Pubmed: 31261711]
Experimental cell research Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts [Pubmed: 31233740]
international journal of molecular sciences Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764]
Archives of Insect Biochemistry and Physiology Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia [Pubmed: 31774317]
BMC Endocrine Disorders The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561]
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
Journal of Applied Microbiology The effects of dietary sodium butyrate supplementation on the growth performance, carcass traits, and intestinal microbiota of growing‐finishing pigs [Pubmed: 32048746]
LIFE SCIENCES Intravitreal conbercept improves outcome of proliferative diabetic retinopathy through inhibiting inflammation and oxidative stress [Pubmed: 33227274]
Metab Brain Dis Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats [Pubmed: 33170419]
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice [Pubmed: 33065235]
BRITISH JOURNAL OF NUTRITION Ginger and turmeric lipid-solubles attenuate heated oil-induced cardio-hepatic oxidative stress via the upregulation of NRF2 and decrease blood pressure in rats [Pubmed: 33028437]
MOLECULAR AND CELLULAR BIOCHEMISTRY MicroRNA-217 ameliorates inflammatory damage of endothelial cells induced by oxidized LDL by targeting EGR1 [Pubmed: 32737769]
OBESITY SURGERY The Protective Effects of Butorphanol on Pulmonary Function of Patients with Obesity Undergoing Laparoscopic Bariatric Surgery: a Double-Blind Randomized … [Pubmed: 32535786]
Inflammation Tetramethylpyrazine Inhibits Platelet Adhesion and Inflammatory Response in Vascular Endothelial Cells by Inhibiting P38 MAPK and NF-κB Signaling Pathways []
J Transl Med Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555]
Molecules Alpha-Lipoic Acid Plays a Role in Endometriosis: New Evidence on Inflammasome-Mediated Interleukin Production, Cellular Adhesion and Invasion [33430011]
Antioxidants (Basel) Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model [33562069]
J Vet Intern Med Association of markers of endothelial activation and dysfunction with occurrence and outcome of pulmonary hemorrhage in dogs with leptospirosis [34076314]
PLoS One A comparison of the effects of monotherapy with rosuvastatin, atorvastatin or ezetimibe versus combination treatment with rosuvastatin-ezetimibe and … [34492054]
Sci. Pharm Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism []
Journal of the South African Veterinary Association Circulating markers of endothelial activation in canine parvoviral enteritis []
留言咨询