巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Macrophage Migration Inhibitory Factor (MIF)

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA698Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测巨噬细胞移动抑制因子(MIF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的巨噬细胞移动抑制因子(MIF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 82-99 87
EDTA plasma(n=5) 84-95 89
heparin plasma(n=5) 96-103 99

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的巨噬细胞移动抑制因子(MIF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中巨噬细胞移动抑制因子(MIF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 79-91% 85-101% 85-96%
EDTA plasma(n=5) 93-102% 87-101% 93-105% 80-97%
heparin plasma(n=5) 87-101% 95-102% 80-97% 94-102%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将巨噬细胞移动抑制因子(MIF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的巨噬细胞移动抑制因子(MIF)与连接于固相载体上的抗体结合，然后加入生物素化的巨噬细胞移动抑制因子(MIF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的巨噬细胞移动抑制因子(MIF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA698Ra01 巨噬细胞移动抑制因子(MIF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA698Ra01 巨噬细胞移动抑制因子(MIF)多克隆抗体 WB; IHC; ICC; IP.
MAA698Ra21 巨噬细胞移动抑制因子(MIF)单克隆抗体 WB; IHC; ICC; IP.
WEA698Ra 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA698Ra 巨噬细胞移动抑制因子(MIF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA698Ra 巨噬细胞移动抑制因子(MIF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Kidney International Targeted reduction of advanced glycation improves renal function in obesity [PubMed: 21412218]
The Journal of Immunology Macrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the Gut [Jimmunol: 5673]
The Journal of Endocrinology Involvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver disease [PubMed: PMC3757527]
The Journal of Immunology Role of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing mice [PubMed: PMC3466372]
Diabetologia Deletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetes [Pubmed:24957662]
BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the Bladder [PubMed: 25705692]
Diabetologia Combined NOX1/4 inhibition with GKT137831 in mice provides dose-dependent reno- and atheroprotection even in established micro- and macrovascular disease. [pubmed:28160092]
Life Sciences Protective effect of chlorogenic acid on the inflammatory damage of pancreas and lung in mice with l-arginine-induced pancreatitis [pubmed:28919396]
Scientific Reports Cytokine MIF Enhances Blood-Brain Barrier Permeability: Impact for Therapy in Ischemic Stroke [Pubmed:29335619]
Ocular Immunology and Inflammation Immune Response and Mechanisms of IFN-γ in Administration for Keratomycosis [Pubmed: 30307777]
International Journal of Immunopathology and Pharmacology Effect of voluntary running on expression of myokines in brains of rats with depression [Pubmed: 30834799]
Biomarkers in Heart Failure and Associated Diseases Biomarkers in Heart Failure and Associated Diseases []
Science China-Life Sciences Autophagy mediates the secretion of macrophage migration inhibitory factor from cardiomyocytes upon serum-starvation [Pubmed: 31209799]
Folia Morphologica Beneficial effects of voluntary over forced exercise on skeletal muscle structure and myokines expression [Pubmed: 31802473]
Biomed Pharmacother Deletion of macrophage migration inhibitory factor ameliorates inflammation in mice model severe acute pancreatitis [Pubmed: 32062385]
Sci Rep Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223]
Commun Biol Macrophage migration inhibitory factor is overproduced through EGR1 in TET2low resting monocytes [Pubmed:35115654]
留言咨询