S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for S100 Calcium Binding Protein A12 (S100A12)

CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6

  • S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB080Rb.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测S100钙结合蛋白A12(S100A12),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的S100钙结合蛋白A12(S100A12)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 79-97 89
EDTA plasma(n=5) 80-103 81
heparin plasma(n=5) 83-96 91

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的S100钙结合蛋白A12(S100A12),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中S100钙结合蛋白A12(S100A12)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 88-101% 85-98% 78-88% 88-101%
EDTA plasma(n=5) 80-94% 88-101% 85-92% 80-102%
heparin plasma(n=5) 81-101% 84-92% 83-104% 91-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将S100钙结合蛋白A12(S100A12)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的S100钙结合蛋白A12(S100A12)与连接于固相载体上的抗体结合,然后加入生物素化的S100钙结合蛋白A12(S100A12)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的S100钙结合蛋白A12(S100A12)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Oryctolagus cuniculus (Rabbit,兔) 应用(仅供研究使用,不用于临床诊断!)
RPB080Rb01 S100钙结合蛋白A12(S100A12)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB080Rb51 S100钙结合蛋白A12(S100A12)多克隆抗体 WB; IHC; ICC; IP.
PAB080Rb01 S100钙结合蛋白A12(S100A12)多克隆抗体 WB; IHC; ICC; IP.
SEB080Rb S100钙结合蛋白A12(S100A12)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB080Rb S100钙结合蛋白A12(S100A12)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB080Rb01 S100钙结合蛋白A12(S100A12)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It(ELISA Kit) Yourself"

参考文献

杂志 参考文献
Inflammation Research Correlation of human S100A12 (EN-RAGE) and high-sensitivity C-reactive protein as gingival crevicular fluid and serum markers of inflammation in chronic periodontitis and type 2 diabetes [Pubmed: 24378957]
Dig Dis Sci The Circulating Level of Soluble Receptor for Advanced Glycation End Products Displays Different Patterns in Ulcerative Colitis and Crohn’s Disease: A Cross-Sectional Study [PubMed: 25757448]
The Journal of Clinical Endocrinology & Metabolism Low Endogenous Secretory Receptor for Advanced Glycation End-Products Levels Are Associated With Inflammation and Carotid Atherosclerosis in Prediabetes [Pubmed:26885882]
Nutrition, Metabolism, and Cardiovascular Diseases High intake of dietary advanced glycation end-products is associated with increased arterial stiffness and inflammation in subjects with type 2 diabetes. [pubmed:28958695]
The Journal of Clinical Endocrinology & Metabolism HbA1c Identifies Subjects With Prediabetes and Subclinical Left Ventricular Diastolic Dysfunction. [pubmed:28973588]
Journal of Immunology Research A Granulocyte-Specific Protein S100A12 as a Potential Prognostic Factor Affecting Aggressiveness of Therapy in Patients with Juvenile Idiopathic Arthritis [Doi: 10.1155/2018/5349837]
Al-Azhar Assiut Medical Journal The role of serum S100A12 (calgranulin C) as a diagnostic marker in Egyptian patients with irritable bowel syndrome and ulcerative colitis [Doi: 10.4103/AZMJ.AZMJ_52_18]
Acta Diabetologica Analysis of S100A12 plasma levels in hyperlipidemic subjects with or without familial hypercholesterolemia [Pubmed: 30963307]
Cells 1 h Postload Glycemia Is Associated with Low Endogenous Secretory Receptor for Advanced Glycation End Product Levels and Early Markers of Cardiovascular … []
Biomolecules . Multimarker Approach to Identify Patients with Coronary Artery Disease at High Risk for Subsequent Cardiac Adverse Events: The Multi-Biomarker Study [Pubmed: 32549327]
Mediators of Inflammation Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris []
Animals (Basel) The Serum and Saliva Proteome of Dogs with Diabetes Mellitus [33271797]
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