Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Salusin Beta (SALb)
- 编号CEC026Ra
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 实验方法竞争抑制
- 反应时长2h
- 检测范围4.94-400pg/mL
- 灵敏度最小可检测剂量小于等于1.94pg/mL.
- 样本类型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下载 英文说明书 中文说明书
- 规格 48T96T 96T*5 96T*10 96T*100
- 价格 ¥ 3192 ¥ 4560 ¥ 20520 ¥ 38760 ¥ 319200
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产品包装(模拟)
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产品包装(模拟)
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实验结果图
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标准曲线图
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测Salusin肽β(SALb)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的Salusin肽β(SALb)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 87-96 | 90 |
| EDTA plasma(n=5) | 95-103 | 101 |
| heparin plasma(n=5) | 86-101 | 93 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的Salusin肽β(SALb)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中Salusin肽β(SALb)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-102% | 90-104% | 97-104% | 88-96% |
| EDTA plasma(n=5) | 80-105% | 86-99% | 89-97% | 79-91% |
| heparin plasma(n=5) | 88-103% | 80-101% | 95-102% | 94-102% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）50µL，
加入50µL检测液A(临用前配制);
37°C温育1小时。
3. 洗板3次；
4. 加检测溶液B100µL，37°C孵育30分钟；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分钟；
7. 加终止液50µL，立即450nm读数。
实验原理
本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将Salusin肽β(SALb)单克隆抗体包被微孔板，制成固相载体，往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本)，待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物，然后加入HRP标记的亲和素，经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。待测标本浓度越高，标记抗原和抗体的结合就越受到抑制，显色愈浅。显色的深浅与酶量呈正相关，而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度（O.D.值），计算样品浓度。
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| CPC026Ra21 | Salusin肽β(SALb)卵白蛋白偶联物 | Immunogen; SDS-PAGE; WB. |
| CEC026Ra | Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| WEC026Ra | Salusin肽β(SALb)检测试剂盒(酶联免疫吸附试验法,宽范围) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC026Ra | Salusin肽β(SALb)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| KSC026Ra11 | Salusin肽β(SALb)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| Journal of Maternal-Fetal and Neonatal Medicine | Serum levels of apelin, salusin-alpha, and salusin-beta in normal pregnancy and preeclampsia [Informa: 14767058.2012.660221] |
| Peptides | The bioactive peptides salusins and apelin–36 are produced in human arterial and venous tissues and the changes of their levels during cardiopulmonary bypass [PubMed: 22884920] |
| Department of Medical Biochemistry and Clinical Biochemistry | Presence of adropin, nesfatin-1, apelin-12, ghrelins and salusins peptides in the milk, cheese whey and plasma of dairy cows [ScienceDirect: S0196978113000533] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Association of low maternal levels of salusins with gestational diabetes mellitus and with small-for-gestational-age fetuses. [PubMed: 23178004] |
| Biotech Histochem. | Serum concentration and kidney expression of salusin-α and salusin-β in rats with metabolic syndrome induced by fructose. [PubMed: 23244237] |
| Gynecological Endocrinology, | Salusins, newly identified regulators of hemodynamics and mitogenesis, increase in polycystic ovarian syndrome. [PubMed: 22827297] |
| Peptides | Concentrations of preptin, salusins and hepcidins in plasma and milk of lactating women with or without gestational diabetes mellitus [ScienceDirect: S0196978113003100] |
| Biotechnic & Histochemistry | Salusin-alpha and -beta expression in heart and aorta with and without metabolic syndrome [Com:Source] |
| Med Sci Monit. | Brain, Liver, and Serum Salusin-alpha and-beta Alterations in Sprague-Dawley Rats with or Without Metabolic Syndrome [Pubmed:Pmc4136931] |
| Acta Physiologica | Superoxide anions involved in sympathoexcitation and pressor effects of salusin-β in paraventricular nucleus in hypertensive rats [Pubmed:24304512] |
| Pediatr Nephrol. | Is the serum level of salusin-β associated with hypertension and atherosclerosis in the pediatric population? [Pubmed:25245503] |
| European Journal of Dermatology | Serum salusin-α and salusin-β levels in patients with psoriasis [PubMed: 26055619] |
| J Investig Med | Serum Salusin-β Levels Are Associated With the Presence and Severity of Coronary Artery Disease [PubMed: 25730454] |
| Genet Test Mol Biomarkers. | Serum Salusin-β Levels Are Correlated with Slow Coronary Flow. [Pubmed:27227272] |
| Sci Rep. | Silencing salusin-β attenuates cardiovascular remodeling and hypertension in spontaneously hypertensive rats. [pubmed:28230187] |
| Cell Death Dis. | Salusin-β contributes to oxidative stress and inflammation in diabetic cardiomyopathy [pubmed:28333148] |
| Oxidative Medicine and Cellular Longevity | Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma [10.1155:2017] |
| International Journal Of Cardiology | Silencing salusin β ameliorates heart failure in aged spontaneously hypertensive rats by ROS-relative MAPK/NF-κB pathways in the paraventricular nucleus [Pubmed: 30581101] |
| Multiple Sclerosis and Related Disorders | Increased level of Plasma Salusin-α and Salusin-β in patients with Multiple Sclerosis [Pubmed: 30738876] |
| cardiology in the young | Salusin-α levels are negatively correlated with diastolic blood pressure in children with obesity [Pubmed: 31486354] |
| Redox Biology | Salusin-β mediates tubular cell apoptosis in acute kidney injury: Involvement of the PKC/ROS signaling pathway [Pubmed: 31884071] |
| Journal of international medical research | Salusin-β is superior to salusin-α as a marker for evaluating coronary atherosclerosis [Pubmed: 32054363] |
| cardiovascular drugs and therapy | A TOR2A Gene Product: Salusin-β Contributes to Attenuated Vasodilatation of Spontaneously Hypertensive Rats. [Pubmed: 32458319] |
| Serum level of salusin β as an indicator of metabolic disorders in Acute Lymphoblastic Leucaemia and Wilms Tumour survivors [] | |
| Mol Med Rep | Salusin‑β participates in high glucose‑induced HK‑2 cell ferroptosis in a Nrf‑2‑dependent manner [34296310] |
