分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Secreted Frizzled Related Protein 5 (SFRP5)

SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b

  • 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEC842Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测分泌型卷曲相关蛋白5(SFRP5),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的分泌型卷曲相关蛋白5(SFRP5)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 94-101 98
EDTA plasma(n=5) 78-96 84
heparin plasma(n=5) 79-98 95

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的分泌型卷曲相关蛋白5(SFRP5),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中分泌型卷曲相关蛋白5(SFRP5)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 95-105% 83-90% 97-105% 87-96%
EDTA plasma(n=5) 81-93% 87-98% 89-103% 91-103%
heparin plasma(n=5) 98-105% 78-90% 80-102% 88-98%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将分泌型卷曲相关蛋白5(SFRP5)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的分泌型卷曲相关蛋白5(SFRP5)与连接于固相载体上的抗体结合,然后加入生物素化的分泌型卷曲相关蛋白5(SFRP5)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的分泌型卷曲相关蛋白5(SFRP5)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPC842Ra01 分泌型卷曲相关蛋白5(SFRP5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC842Ra01 分泌型卷曲相关蛋白5(SFRP5)多克隆抗体 WB; IHC; ICC; IP.
LAC842Ra71 分泌型卷曲相关蛋白5(SFRP5)多克隆抗体(生物素标记) WB; IHC; ICC.
MAC842Ra21 分泌型卷曲相关蛋白5(SFRP5)单克隆抗体 WB; IHC; ICC; IP.
SEC842Ra 分泌型卷曲相关蛋白5(SFRP5)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC842Ra 分泌型卷曲相关蛋白5(SFRP5)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Diabetes Research and Clinical Practice Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance [PubMed: 23290274]
European Journal of Clinical Investigation Sfrp5 correlates with insulin resistance and oxidative stress [PubMed: 23398169]
Pediatric Research Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children [Pubmed: 24603290]
Clin Endocrinol (Oxf). SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children [Pubmed: 24330025]
The Journal of Clinical Endocrinology & Metabolism Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans [Pubmed: 23185036]
Atherosclerosis Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease [Pubmed: 24530778]
PLoS One. Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation [Pubmed:Pmc4072672]
J Clin Endocrinol Metab. Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation [Pubmed:24840810]
Oncology Letters Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma [Pubmed:25120720]
Diabetes Metab Res Rev. Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls [Pubmed:25139699]
Clinical &amp; Experimental Immunology The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis [Pubmed:25382802]
Diabetes Metab Res Rev Circulating SFRP5 levels are elevated in drug-na [PubMed: 25139699]
J Matern Fetal Neonatal Med Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels [PubMed: 26100762]
Eur J Clin Invest Sfrp5 associates with beta-cell function in humans [Pubmed:27019073]
Osteoporosis International Bone marrow sFRP5 level is negatively associated with bone formation markers [pubmed:27986984]
Diabetes/Metabolism Research and Reviews Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults [pubmed:28303637]
Cardiovascular Diabetology Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study [pubmed:28851362]
Arteriosclerosis, Thrombosis, and Vascular Biology  Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … [Pubmed:29674475]
Obesity Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans [Pubmed:29427376]
J Bas Res Med Sci Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats [article-1-352-en.pdf]
Molecular Biology Reports The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome [Pubmed: 30194556]
Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling []
British Journal Of Nutrition A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile [Pubmed: 30375290]
J Clin Endocrinol Metab Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542]
Frontiers in Endocrinology Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients [Pubmed: 32300333]
CLINICAL BIOCHEMISTRY Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773]
Sao Paulo Medical Journal Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086]
Kidney Blood Press Res Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway [34469882]
Cytokine Association of serum uromodulin with adipokines in dependence of type 2 diabetes [34920231]
PLoS One Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470]
JMIR Res Protoc Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition … [Pubmed:35475790]
Diabetology Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop … []
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