硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Selenoprotein P1, Plasma (SEPP1)

SEPP; SeP; SEP-P1; SELP

  • 硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB809Ra.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测硒蛋白P1(SEPP1)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的硒蛋白P1(SEPP1)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 86-95 90
EDTA plasma(n=5) 96-105 102
heparin plasma(n=5) 83-90 86

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的硒蛋白P1(SEPP1)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中硒蛋白P1(SEPP1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 82-96% 89-96% 81-105% 98-105%
EDTA plasma(n=5) 86-101% 80-96% 93-105% 96-104%
heparin plasma(n=5) 87-94% 91-101% 80-99% 90-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将硒蛋白P1(SEPP1)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的硒蛋白P1(SEPP1)与连接于固相载体上的抗体结合，然后加入生物素化的硒蛋白P1(SEPP1)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的硒蛋白P1(SEPP1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
RPB809Ra01 硒蛋白P1(SEPP1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB809Ra01 硒蛋白P1(SEPP1)多克隆抗体 WB; IHC; ICC; IP.
MAB809Ra21 硒蛋白P1(SEPP1)单克隆抗体 WB; IHC; ICC; IP.
SEB809Ra 硒蛋白P1(SEPP1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB809Ra 硒蛋白P1(SEPP1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
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Journal of Pediatric Biochemistry Trace elements, heavy metals and vitamins in Egyptian school children with iron deficiency anemia [Metapress:Source]
Journal of Trace Elements in Medicine and Biology Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto&#039;s thyroiditis and hypothyroidism [PubMed: 26854239]
OBES RES CLIN PRACT/OBESITY RESEARCH & CLINICAL PRACTICE Selenoprotein P is elevated in individuals with obesity, but is not independently associated withinsulin resistance. [pubmed:27524654]
Annals of Nutrition and Metabolism Acute Overfeeding Does Not Alter Liver or Adipose Tissue-Derived Cytokines in HealthyHumans. [pubmed:27832637]
European Journal of Nutrition DNA damage and oxidative stress response to selenium yeast in the non-smoking individuals: ashort-term supplementation trial with respect to GPX1 and SEPP1 polymorphism. [pubmed:26658762]
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Biological & Pharmceutical Bulletin Comparison of Human Selenoprotein P Determinants in Serum between Our Original Methods and Commercially Available Kits [Pubmed:29709922]
Diabetologia Non-alcoholic fatty liver disease in the first trimester and subsequent development of gestational diabetes mellitus [Pubmed: 30470912]
Biological Trace Element Research Selenium Levels in Community Dwellers with Type 2 Diabetes Mellitus [Pubmed: 30725267]
Advances in Medical Sciences Can hepatokines be regarded as novel non-invasive serum biomarkers of intrahepatic lipid content in obese children? [Pubmed: 30921653]
Acute swimming exercise, but not exposure to moderate hypoxic conditions reduces circulating selenoprotein P levels in short-term, high-fat diet-fed rats [Pubmed: 31236416]
Hormones Selenium and selenoprotein P in nonalcoholic fatty liver disease [Pubmed: 31493247]
Bull Environ Contam Toxicol Soil Selenium Concentration and Residents Daily Dietary Intake in a Selenosis Area: A Preliminary Study in Yutangba Village, Enshi City, China [Pubmed: 32909074]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Higher Serum Selenoprotein P Level as a Novel Inductor of Metabolic Complications in Psoriasis [Pubmed: 32605214]
Circulating hepassocin level in patients with stable angina is associated with fatty liver and renal function []
Nat Immunol Selenium–GPX4 axis protects follicular helper T cells from ferroptosis [34413521]
Reviews in Cardiovascular Medicine Selenoprotein P-1 (SEPP1) as An Early Biomarker of Acute Kidney Injury in Patients Undergoing Cardiopulmonary Bypass []
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