细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Suppressors Of Cytokine Signaling 3 (SOCS3)

ATOD4; CIS3; Cish3; MGC71791; SOCS-3; SSI-3; SSI3; Cytokine-inducible SH2 protein 3; STAT-induced STAT inhibitor 3

  • 细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEB684Bo.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测细胞因子信号转导抑制因子3(SOCS3),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将细胞因子信号转导抑制因子3(SOCS3)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的细胞因子信号转导抑制因子3(SOCS3)与连接于固相载体上的抗体结合,然后加入生物素化的细胞因子信号转导抑制因子3(SOCS3)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的细胞因子信号转导抑制因子3(SOCS3)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
SEB684Bo 细胞因子信号转导抑制因子3(SOCS3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
The Journal of Biological Chemistry Human primary keratinocytes show a restricted ability to up-regulate suppressor of cytokine signaling (SOCS)3 compared to autologous macrophages [Jcb: 280370]
The Journal of Biological Chemistry Human Primary Keratinocytes Show Restricted Ability to Up-regulate Suppressor of Cytokine Signaling (SOCS)3 Protein Compared with Autologous Macrophages [PubMed: PMC3323061]
Journal of Reproductive Immunology What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? [Pubmed: 24368037]
The Journal of Immunology Alveolar Epithelial Cell–Derived Prostaglandin E2 Serves as a Request Signal for Macrophage Secretion of Suppressor of Cytokine Signaling 3 during Innate Inflammation [Pubmed:27183597]
Scientific Reports The FXR Agonist, Obeticholic Acid, Suppresses HCC Proliferation & Metastasis: Role of IL-6/STAT3 Signalling Pathway [pubmed:28970500]
Journal of Immunological Methods Endogenous peroxidases in sputum interfere with horse-radish peroxidase-based ELISAs. [pubmed:29154772]
Turkish Journal Of Medical Sciences The impact of plasma SOCS3 levels and endometrial leukocytes on unexplained infertility [Pubmed:29914245]
Novel trajectories of Bromocriptine antidiabetic action: Leptin-IL-6/JAK2/p-STAT3/SOCS3, p-IR/p-AKT/GLUT4, PPARγ/Adiponectin, Nrf2/PARP-1, and GLP-1 [articles:10.3389]
JCI Insight Alveolar macrophage secretion of vesicular SOCS3 represents a platform for lung cancer therapeutics [Pubmed: 31619584]
European Journal of Pharmacology Olmesartan attenuates type 2 diabetes-associated liver injury: Cross-talk of AGE/RAGE/JNK, STAT3/SCOS3 and RAS signaling pathways [Pubmed: 32067934]
FASEB JOURNAL Resident alveolar macrophage‐derived vesicular SOCS3 dampens allergic airway inflammation [Pubmed: 32030817]
molecular immunology SOCS3-deficient lung epithelial cells uptaking neutrophil-derived SOCS3 worsens lung influenza infection [Pubmed: 32645550]
Experimental and Therapeutic Medicine Changes and clinical significance of serum inflammatory factors in the treatment of pregnancy hypertension syndrome with magnesium sulfate combined with … [Pubmed: 32742411]
NEUROCHEMICAL RESEARCH Modulation of the Nitric Oxide/BH4 Pathway Protects Against Irradiation-Induced Neuronal Damage [33755856]
BIOMEDICINE & PHARMACOTHERAPY Potential contribution of ELISA and LFI Assays to assessment of the oxidative stress condition based on 8-oxodG biomarker [33957564]
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