血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法)

ELISA Kit for Von Willebrand Factor (vWF)

F8VWF; VWD; von Willebrand antigen 2

  • 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法) 产品包装(模拟)
  • 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法) 实验结果图
  • SEA833Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测血管性血友病因子(vWF)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的血管性血友病因子(vWF)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
sodium citrate plasma(n=5) 79-93 87

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的血管性血友病因子(vWF)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中血管性血友病因子(vWF)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 87-99% 94-103% 85-96% 88-98%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备；
2. 加样（标准品及样本）100µL，37°C孵育1小时；
3. 吸弃，加检测溶液A100µL，37°C孵育1小时；
4. 洗板3次；
5. 加检测溶液B100µL，37°C孵育30分钟；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分钟；
8. 加终止液50µL，立即450nm读数。

实验原理

将血管性血友病因子(vWF)抗体包被于96孔微孔板中，制成固相载体，向微孔中分别加入标准品或标本，其中的血管性血友病因子(vWF)与连接于固相载体上的抗体结合，然后加入生物素化的血管性血友病因子(vWF)抗体，将未结合的生物素化抗体洗净后，加入HRP标记的亲和素，再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管性血友病因子(vWF)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值)，计算样品浓度。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPA833Hu01 血管性血友病因子(vWF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA833Hu21 血管性血友病因子(vWF)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA833Hu01 血管性血友病因子(vWF)多克隆抗体 WB; IHC; ICC; IP.
PAA833Hu08 血管性血友病因子(vWF)多克隆抗体 WB; IHC; ICC; IP.
LAA833Hu71 血管性血友病因子(vWF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA833Hu22 血管性血友病因子(vWF)单克隆抗体 WB; IHC; ICC; IP.
SEA833Hu 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA833Hu 血管性血友病因子(vWF)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA833Hu 血管性血友病因子(vWF)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA833Hu11 血管性血友病因子(vWF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Int J Nanomedicine. Amorphous silica nanoparticles impair vascular homeostasis and induce systemic inflammation [Pubmed:Pmc4047982]
Inflammation.? Systemic and Flap Inflammatory Response Associates with Thrombosis in Flap Venous Crisis [Pubmed:25448261]
Cellular Physiology and Biochemistry Short-Term Nose-Only Water-Pipe (Shisha) Smoking Exposure Accelerates Coagulation and Causes Cardiac Inflammation and Oxidative Stress in Mice [Pubmed:25634761]
Asian Pac J Trop Med Blood pH in coronary artery microthrombosis of rats [PubMed: 26522304]
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880]
Sci Rep. Differential integrative omic analysis for mechanism insights and biomarker discovery of abnormal Savda syndrome and its unique Munziq prescription [Pubmed:27296761]
American Journal of Reproductive Immunology Circulating endothelial cells, circulating endothelial progenitor cells, and von Willebrand factor in pregnancies complicated by hypertensive disorders [doi:10.1111]
Shock Low-Dose Sodium Nitrite Fluid Resuscitation Prevents Lethality From Crush Syndrome by Improving Nitric Oxide Consumption and Preventing Myoglobin Cytotoxicity in Kidney in A Rat Model. [pubmed:27941593]
Regenerative Biomaterials The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic [10.1093/rb/rbx028]
Acta Anaesthesiologica Scandinavica Early prognostic factors in septic shock cancer patients: a prospective study with a proteomic approach [Pubmed:29315472]
Gynecologic and obstetric investigation Relationship between the von Willebrand Factor Plasma Concentration and Ultrasonographic Doppler Findings in Pregnancies Complicated by Hypertensive … [Pubmed:29621786]
Biological Trace Element Research Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020]
Experimental and Therapeutic Medicine Risk stratification and prognostic evaluation of endothelial cell‑specific molecule1, von Willebrand factor, and a disintegrin‑like and metalloprotease with … []
Scientific Reports Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827]
International Journal of Molecular Sciences Waterpipe Tobacco Smoke Inhalation Triggers Thrombogenicity, Cardiac Inflammation and Oxidative Stress in Mice: Effects of Flavouring [Pubmed: 32075078]
environmental research Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827]
evidence-based complementary and alternative medicine Xiaoyukang Jiaonang Promotes the Degradation of Hypoxia-Inducible Factor 1α and Antiangiogenesis and Anti-Inflammation in Chronic Subdural Hematoma … [Pubmed: 32228124]
Indian Journal of Public Health Research & Development Enhanced Fibrin-Lysis in Grade-1 Dengue Haemorrhagic Fever []
Ad hatoda Vasica: a potential ayurvedic intervention against COVID-19 associated impaired immune response and hypoxia-inflammation phenotype []
Radiat Res Investigating the Multi-Faceted Nature of Radiation-Induced Coagulopathies in a G?ttingen Minipig Model of Hematopoietic Acute Radiation Syndrome [34019667]
Intensive Care Medicine Experimental Preservation of renal endothelial integrity and reduction of renal edema by aprotinin does not preserve renal perfusion and function following experimental … [34169407]
Experimental and Therapeutic Medicine Salvianolic acid B improves the survival rate, acute kidney dysfunction, inflammation and NETosis‑mediated antibacterial action in a crush syndrome rat model [Pubmed:35386617]
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