亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法,高敏型)

High Sensitive ELISA Kit for Cyclophilin A (CYPA)

PPIA; CyP-A; CYPH; Peptidylprolyl Isomerase A; Peptidyl-Prolyl Isomerase A; Peptidyl-prolyl cis-trans isomerase A; Cyclosporin A-binding protein

  • 亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法,高敏型) 产品包装(模拟)
  • 亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法,高敏型) 产品包装(模拟)
  • 亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法,高敏型) 实验结果图
  • HEA979Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测亲环素A(CYPA),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的亲环素A(CYPA)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-92 88
EDTA plasma(n=5) 85-101 95
heparin plasma(n=5) 89-102 98

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的亲环素A(CYPA),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中亲环素A(CYPA)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 87-98% 97-105% 80-101%
EDTA plasma(n=5) 88-96% 79-98% 92-105% 79-101%
heparin plasma(n=5) 95-102% 84-104% 95-102% 98-105%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将亲环素A(CYPA)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的亲环素A(CYPA)与连接于固相载体上的抗体结合,然后加入生物素化的亲环素A(CYPA)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的亲环素A(CYPA)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA979Mu01 亲环素A(CYPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA979Mu02 亲环素A(CYPA)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA979Mu02 亲环素A(CYPA)多克隆抗体 WB; IHC; ICC; IP.
PAA979Mu01 亲环素A(CYPA)多克隆抗体 WB; IHC; ICC; IP.
LAA979Mu81 亲环素A(CYPA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA979Mu71 亲环素A(CYPA)多克隆抗体(生物素标记) WB; IHC; ICC.
HEA979Mu 亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA979Mu 亲环素A(CYPA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
PSA979Mu01 亲环素A(CYPA)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA979Mu01 亲环素A(CYPA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Atherosclerosis EMMPRIN and its ligand cyclophilin A regulate MT1-MMP, MMP-9 and M-CSF during foam cell formation [PubMed: 19758589]
Biomarkers Cyclophilin-A: a novel biomarker for untreated male essential hypertension [Pubmed: 24164594]
Cardiovascular Diabetology Plasma level of cyclophilin A is increased in patients with type 2 diabetes mellitus and suggests presence of vascular disease [Pubmed:24502618]
International Journal of Biological Sciences Decrease in Plasma Cyclophilin A Concentration at 1 Month after Myocardial Infarction Predicts Better Left Ventricular Performance and Synchronicity at 6 Months: A Pilot Study in Patients with ST Elevation Myocardial Infarction [Pubmed:25552928]
Medicine Cyclophilin A in Ruptured Intracranial Aneurysm [PubMed: 26426668]
Mol Cell Biochem PPIA rs6850: A> G single-nucleotide polymorphism is associated with raised plasma cyclophilin A levels in patients with coronary artery disease [PubMed: 26702934]
Medicine (Baltimore) Urinary Cyclophilin A as a New Marker for Diabetic Nephropathy: A Cross-Sectional Analysis of Diabetes Mellitus [PubMed: 26496315]
International Journal of Cardiology Cyclophilin A modulates bone marrow-derived CD117(+) cells and enhances ischemia-induced angiogenesisvia the SDF-1/CXCR4 axis. [Pubmed:27057951]
Clinica Chimica Acta Novel findings of secreted cyclophilin A in diabetic nephropathy and its association with renal protection of dipeptidyl peptidase 4 inhibitor [pubmed:27823952]
Cardiovascular Diabetology Cyclophilin A enhances macrophage differentiation and lipid uptake in high glucose conditions: a cellular mechanism for accelerated macro vascular disease in diabetes mellitus. [pubmed:27809851]
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