高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法,快捷型)

Instant ELISA Kit for High Mobility Group Protein 1 (HMG1)

HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

  • 高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法,快捷型) 产品包装(模拟)
  • 高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法,快捷型) 产品包装(模拟)
  • 高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法,快捷型) 实验结果图
  • IEA399Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测高迁移率族蛋白1(HMG1),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的高迁移率族蛋白1(HMG1)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 96-103 99
EDTA plasma(n=5) 79-93 82
heparin plasma(n=5) 98-105 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的高迁移率族蛋白1(HMG1),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中高迁移率族蛋白1(HMG1)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 94-101% 79-91% 85-92% 83-96%
EDTA plasma(n=5) 83-90% 86-95% 81-99% 79-98%
heparin plasma(n=5) 89-98% 89-98% 96-105% 91-99%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

试剂盒内容

试剂名称 数量 试剂名称 数量
96孔板(预包被) 1 96孔板覆膜 4
标准品 2 标准品稀释液 1×20mL
检测溶液A 1×120µL 检测稀释液A 1×12mL
检测溶液B 1×120µL 检测稀释液B 1×12mL
TMB底物 1×9mL 终止液 1×6mL
浓洗涤液 (30 × concentrate) 1×20mL 说明书 1

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

Instant ELISA Kit for High Mobility Group Protein 1 (HMG1)

实验原理

将高迁移率族蛋白1(HMG1)单克隆抗体包被于96孔微孔板中,制成固相载体,向微孔中加入标准品或标本以及同时加入HRP标记高迁移率族蛋白1(HMG1)的多克隆抗体,孵育结束彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的被检测物浓度呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA399Hu02 高迁移率族蛋白1(HMG1)活性蛋白 Cell culture; Activity Assays.
EPA399Hu61 高迁移率族蛋白1(HMG1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA399Hu02 高迁移率族蛋白1(HMG1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA399Hu01 高迁移率族蛋白1(HMG1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA399Hu06 高迁移率族蛋白1(HMG1)多克隆抗体 WB; IHC; ICC; IP.
PAA399Hu01 高迁移率族蛋白1(HMG1)多克隆抗体 WB; IHC; ICC; IP.
PAA399Hu02 高迁移率族蛋白1(HMG1)多克隆抗体 WB; IHC; ICC; IP.
LAA399Hu71 高迁移率族蛋白1(HMG1)多克隆抗体(生物素标记) WB; IHC; ICC.
LAA399Hu81 高迁移率族蛋白1(HMG1)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA399Hu22 高迁移率族蛋白1(HMG1)单克隆抗体 WB; IHC; ICC; IP.
SEA399Hu 高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA399Hu 高迁移率族蛋白1(HMG1)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA399Hu 高迁移率族蛋白1(HMG1)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA399Hu01 高迁移率族蛋白1(HMG1)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料
PGA399Hu01 高迁移率族蛋白1(HMG1)引物对 PCR

参考文献

杂志 参考文献
Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine High mobility group box protein-1 (HMGB-1) as a new diagnostic marker in patients with acute appendicitis [PubMed: PMC3094252]
Respiration Protective Effect of Nicotine on Lipopolysaccharide-Induced Acute Lung Injury in Mice [Karger: 000319151]
Surgery Clinical relevance of single nucleotide polymorphisms of the high mobility group box 1 protein gene in patients with major trauma in Southwest China [Pubmed: 22047946]
Viral Immunology Is HMGB1 a New Indirect Marker for Revealing Fibrosis in Chronic Hepatitis and a New Therapeutic Target in Treatment? [Wiley: source]
Chinese Medical Journal Severity of sepsis is correlated with the elevation of serum high-mobility group box 1 in rats [CMJ: 2009218808935901080]
Microbiology and Immunology Diagnostic value of serum concentrations of high-mobility group-box protein 1 and soluble hemoglobin scavenger receptor in brucellosis [PubMed: 23278480]
ACTA biochimica polonica High mobility group B1 levels in sepsis and disseminated intravascular coagulation [Actabp: Source]
Anesthesia and Analgesia The Inhibitory Effect of Lidocaine on the Release of High Mobility Group Box 1 in Lipopolysaccharide-Stimulated Macrophages [PubMed: 21288974]
Rheumatology International Elevated serum levels of high mobility group box protein 1 (HMGB1) in patients with ankylosing spondylitis and its association with disease activity and quality of life [PubMed: 23143556]
Inflammation Research Mammalian target of rapamycin complex 2 regulates inflammatory response to stress [PubMed: 22899279]
Acta Biochimica et Biophysica Sinica IGF-1 alleviates ox-LDL-induced inflammation via reducing HMGB1 release in HAECs [PubMed: 22782142]
LabVest The suppression of TRIM21 and the accumulation of IFN-α play crucial roles in the pathogenesis of osteonecrosis of the femoral head. [PubMed: 22825687]
Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
Evidence-based Complementary and Alternative Medicine ATF3 Protects against LPS-Induced Inflammation in Mice via Inhibiting HMGB1 Expression [PubMed: PMC3770047]
Exp Ther Med Correlation between serum high-mobility group box-1 levels and high-sensitivity C-reactive protein and troponin I in patients with coronary artery disease [Pubmed: 23935732]
International of Rheumatic disesases Serum levels of high mobility group box 1 protein and its association with quality of life and psychological and functional status in patients with fibromyalgia [Pubmed: 23901875]
Surgery A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC) [Pubmed: 24468034]
Acta Pharmacol Sin. Simvastatin suppresses vascular inflammation and atherosclerosis in ApoE-/- mice by downregulating the HMGB1-RAGE axis [Pubmed: 23564080]
BMC pulmonary medicine Soluble receptor for advanced glycation end-products and progression of airway disease [Pubmed: 24758342]
Biochemical Pharmacology Ascorbic acid reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and improves survival rate in septic mice by activation of Nrf2/HO-1 signals [Pubmed:25896849]
BioMed Research International Effects of Single or Combined Treatments with Radiation and Chemotherapy on Survival and Danger Signals Expression in Glioblastoma Cell Lines [Hindawi:Source]
J Cardiothorac Surg. High plasma levels of high mobility group box 1 is associated with the risk of sepsis in severe blunt chest trauma patients: a prospective cohort study [Pubmed:Pmc4132233]
J Leukoc Biol. HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells [Pubmed:24374967]
PLOS ONE The Alarmin Concept Applied to Human Renal Transplantation: Evidence for a Differential Implication of HMGB1 and IL-33 [Pubmed:24586382]
Journal of Surgical Research Plasma levels of high mobility group box 1 increase in patients with posttraumatic stress disorder after severe blunt chest trauma: a prospective cohort study [Pubmed:25016440]
Virus Research High-mobility group box-1, promising serological biomarker for the distinction of human WNV disease severity [Pubmed:25197038]
Journal of Stroke and Cerebrovascular Diseases Increased VEGF and Decreased SDF-1α in Patients with Silent Brain Infarction Are Associated with Better Prognosis after First-Ever Acute Lacunar Stroke [Pubmed:25601176]
中华临床医师杂志 高迁移率族蛋白B1和神经元烯醇化酶对心脏骤停大鼠复苏后脑损伤评价的研究 [Journal: Fulltext]
Oxidative Medicine and Cellular Longevity Diabetes-Induced Oxidative Stress in Endothelial Progenitor Cells May Be Sustained by a Positive Feedback Loop Involving High Mobility Group Box-1 [Journals: Omcl]
Int Immunopharmacol Hesperidin ameliorates lipopolysaccharide-induced acute lung injury in mice by inhibiting HMGB1 release [PubMed: 25724384]
Journal of Immunotherapy Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity [PubMed: 25962106]
Atherosclerosis Retinoic acid inhibits tissue factor and HMGB1 via modulation of AMPK activity in TNF-α activated endothelial cells and LPS-injected mice [PubMed: 26116962]
PLOS ONE Plasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in China [PubMed: 26317615]
Int Immunopharmacol Betulin attenuates lung and liver injuries in sepsis [PubMed: 26644168]
journal of cardiochoracic surgery High mobility group box 1 gene polymorphism is associated with the risk of postoperative atrial fibrillation after coronary artery bypass surgery [PubMed: 26109393]
Int Immunopharmacol Glycyrrhizin reduces HMGB1 secretion in lipopolysaccharide-activated RAW 264.7 cells and endotoxemic mice by p38/Nrf2-dependent induction of HO-1 [PubMed: 25812767]
World J Gastroenterol Overexpression of HMGB1 A-box reduced lipopolysaccharide-induced intestinal inflammation via HMGB1/TLR4 signaling in vitro [PubMed: 26167076]
Neurotoxicology Allopurinol reduces severity of delayed neurologic sequelae in experimental carbon monoxide toxicity in rats [PubMed: 25845300]
Journal of Neuroimmunology HMGB1 expression patterns during the progression of experimental autoimmune encephalomyelitis [PubMed: 25773152]
J Neuroinflammation HMGB1 release triggered by the interaction of live retinal cells and uveitogenic T cells is Fas/FasL activation-dependent [PubMed: 26394985]
PLoS One Low Shear Stress Induced HMGB1 Translocation and Release via PECAM-1/PARP-1 Pathway to Induce Inflammation Response [PubMed: 25793984]
Neuroscience Interleukin-33 is released in spinal cord and suppresses experimental autoimmune encephalomyelitis in mice [PubMed: 26363151]
Cancer Biomarkers Serum levels of HMGB1 have a diagnostic role in metastatic renal cell cancer [Pubmed:27062570]
International Immunopharmacology Oxymatrine attenuates CCl 4-induced hepatic fibrosis via modulation of TLR4-dependent inflammatory and TGF-β1 signaling pathways [Pubmed:27179304]
SpringerPlus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway. [Pubmed:27026909]
Acta Biochim Biophys Sin (Shanghai). High mobility group box1 protein is involved in acute inflammation induced by Clostridium difficile toxin A. [content:2016]
United States Patent Application COMPOSITIONS AND METHODS FOR INHIBITING HMGB1 ACTIVATION OF MELANOCYTES [y2016:0077082.html]
United States Patent Application METHOD OF MAKING A SKIN CARE COMPOSITION [y2016:0077080.html]
Oxid Med Cell Longev. Oxidative Stress in Metabolic Disorders: Pathogenesis, Prevention, and Therapeutics [Pubmed:26949450]
Oncotarget Zoledronic acid-encapsulating self-assembling nanoparticles and doxorubicin: a combinatorial approach to overcome simultaneously chemoresistance and immunoresistance in breast tumors [pubmed:26980746]
clinical biochemistry Relevance of pre-analytical blood management on the emerging cardiovascular protein biomarkers TWEAK and HMGB1 and on miRNA serum and plasma profiling. [pubmed:27847340]
disease markers Serum HMGB1 as a Potential Biomarker for Patients with Asbestos-Related Diseases. [pubmed:28348451]
Biogerontology. Changes in pro-inflammatory markers and leucine concentrations in response to Nordic Walking training combined with vitamin D supplementation in elderly women. [pubmed:28316011]
Journal of Immunology Research Serum HMGB1 serves as a novel laboratory indicator reflecting disease activity and [pubmed:27800496]
Springerplus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway [pubmed:27026909]
Molecular Immunology HMGB1 blockade differentially impacts pulmonary inflammation and defense responses in poly (I: C)/LPS-exposed heart transplant mice [pubmed:27387278]
Oxidative Medicine and Cellular Longevity Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in … [pubmed:27833703]
BioMed Research International  High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A [pubmed:27579314]
Cardiology The role of high mobility group box 1 protein in interleukin-18-induced myofibroblastic transition of valvular interstitial cells [pubmed:27395056]
Innate immunity HMGB1 exacerbates experimental mouse colitis by enhancing innate lymphoid cells 3 inflammatory responses via promoted IL-23 production [pubmed:27670944]
Urolithiasis. Urinary MCP-1、HMGB1 increased in calcium nephrolithiasis patients and the influence ofhypercalciuria on the production of the two cytokines. [pubmed:27393275]
Journal of Investigative Surgery Diagnostic Value of High-Mobility Group Box 1 (HMGB1) Protein in Acute and Perforated Appendicitis. [pubmed:27922766]
J Cell Biochem High Mobility Group Box 1 Mediates Interferon-r-Induced Phenotypic Modulation of Vascular Smooth Muscle Cells. [pubmed:27579780]
BMC Vet Res. Blood markers of fibrinolysis and endothelial activation in canine babesiosis. [pubmed:28363279]
Immunol Res. Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells. [pubmed:28108937]
Biomed Pharmacother. SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311]
Sci Rep.  Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats. [pubmed:28373667]
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