甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型)

Instant ELISA Kit for Thyroxine (T4)

3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine

  • 甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型) 产品包装(模拟)
  • 甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型) 产品包装(模拟)
  • 甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型) 实验结果图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 78-98 90
EDTA plasma(n=5) 78-104 84
heparin plasma(n=5) 88-97 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 92-99% 85-93% 78-99% 80-103%
EDTA plasma(n=5) 78-90% 83-94% 89-102% 81-96%
heparin plasma(n=5) 80-101% 98-105% 88-104% 79-96%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C温育30分钟;
3. 甩干,加检测溶液A100µL,37°C温育30分钟;
4. 洗板3次;
5. 加检测溶液B100µL, 37°C孵育10分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型)单克隆抗体包被于96孔微孔板中,制成固相载体,向微孔中加入标准品或标本以及同时加入HRP标记甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型)的多克隆抗体,孵育结束彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的被检测物浓度呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA452Ge11 甲状腺素(T4)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA452Ge21 甲状腺素(T4)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA452Ge01 甲状腺素(T4)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA452Ge81 甲状腺素(T4)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
MAA452Ge28 甲状腺素(T4)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA452Ge25 甲状腺素(T4)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA452Ge23 甲状腺素(T4)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA452Ge22 甲状腺素(T4)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA452Ge21 甲状腺素(T4)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA452Ge 甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
IEA452Ge 甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA452Ge 甲状腺素(T4)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CCA452Ge 甲状腺素(T4)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA452Ge 甲状腺素(T4)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA452Ge11 甲状腺素(T4)检测试剂盒DIY材料(酶联免疫吸附试验法) 自己动手制作ELISA试剂盒的主要材料

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