聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Aggrecan (AGC) ,etc. by FLIA (Flow Luminescence Immunoassay)

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

（注：单次混测多因子不超过8个指标 ）

  • 聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 91-98 94
heparin plasma(n=5) 85-92 88
sodium citrate plasma(n=5) 83-96 90

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%

线性

在定值血清及血浆样本内加入适量的聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 89-98% 90-99% 93-105% 93-104%
EDTA plasma(n=5) 78-91% 86-95% 84-98% 97-105%
heparin plasma(n=5) 78-91% 78-91% 78-101% 85-97%
sodium citrate plasma(n=5) 78-94% 88-95% 94-102% 80-101%

稳定性

经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
    37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。

实验原理

将聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法)呈正相关。

赠品

相关产品

编号 适用物种：Oryctolagus cuniculus (Rabbit，兔) 应用(仅供研究使用，不用于临床诊断！)
RPB908Rb01 聚集蛋白聚糖(AGC)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB908Rb51 聚集蛋白聚糖(AGC)多克隆抗体 WB; IHC; ICC; IP.
MAB908Rb21 聚集蛋白聚糖(AGC)单克隆抗体 WB; IHC; ICC; IP.
SEB908Rb 聚集蛋白聚糖(AGC)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB908Rb 聚集蛋白聚糖(AGC)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
Experimental Biology and Medicine Acidic pH conditions mimicking degenerative intervertebral discs impair the survival and biological behavior of human adipose-derived mesenchymal stem cells [Rsmjournals: Source]
Age( dordr). In vitro tenocyte metabolism in aging and oestrogen deficiency [Springer: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Stem Cell Research & Therapy Protein synthesis and secretion in human mesenchymal cells derived from bone marrow, adipose tissue and Wharton's jelly. [Pubmed: 24739658]
Materials Science and Engineering: C Co-electrospun gelatin-poly(l-lactic acid) scaffolds: Modulation of mechanical properties and chondrocyte response as a function of composition [ScienceDirect: S0928493113006656]
PLoS One. Mesenchymal stromal cell proliferation, gene expression and protein production in human platelet-rich plasma-supplemented media [Pubmed:Pmc4130592]
Connective Tissue Research Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747]
Macromolecular Bioscience Highly Porous Gelatin Reinforced 4D Scaffolds for Articular Cartilage Regeneration [PubMed: 25787871]
Osteoarthritis and Cartilage Parathyroid hormone(1-34) exhibits more comprehensive effects than celecoxib in cartilage metabolism and maintaining subchondral bone micro-architecture in meniscectomized Cavia (Guinea pig )inea pigs [Pubmed:26802547]
Cell Proliferation Effects of low oxygen tension on gene profile of soluble growth factors in co‐cultured adipose‐derived stromal cells and chondrocytes [Pubmed:27090063]
bmc musculoskeletal disorders Strontium ranelate causes osteophytes overgrowth in a model of early phase osteoarthritis. [pubmed:28187731]
Journal of Clinical Medicine Aggrecan Turnover in Women with Rheumatoid Arthritis Treated with TNF-α Inhibitors [Pubmed: 32392807]
Loss of autophagy in tibial plateau chondrocytes causes increased apoptosis of chondrocytes in spontaneous osteoarthritis of guinea pigs []
researchsquare Aggrecan-a new biomarker for acute type A aortic dissection [33990642]
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