中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)
Multiplex Assay Kit for Neutrophil Elastase (NE) ,etc. by FLIA (Flow Luminescence Immunoassay)
ELANE; HLE; HNE; ELA2; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Leukocyte Elastase; PMN Elastase
（注：单次混测多因子不超过8个指标 ）
- 编号LMA181Ca
- 物种Canis familiaris; Canine (Dog，犬) 相同的名称，不同的物种。
- 实验方法双抗夹心
- 反应时长3.5h
- 检测范围1.95-2000pg/mL
- 灵敏度最小可检测剂量小于等于0.65 pg/mL.
- 样本类型serum, plasma and other biological fluids
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产品包装(模拟)
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产品包装(模拟)
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通过ISO 9001、ISO 13485质量体系认证
特异性
本试剂盒用于检测中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)，经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制，不可能完成对所有相关或相似物质交叉反应检测，因此本试剂盒有可能与未经检测的其它物质有交叉反应。
回收率
分别于定值血清及血浆样本中加入一定量的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)（加标样品），重复测定并计算其均值，回收率为测定值与理论值的比率。
| 样本 | 回收率范围(%) | 平均回收率(%) |
| serum(n=5) | 81-93 | 85 |
| EDTA plasma(n=5) | 90-97 | 93 |
| heparin plasma(n=5) | 78-88 | 82 |
精密度
精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差：取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次，分别计算不同浓度样本的平均值及SD值。
批间差：选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定，每个样本使用同一试剂盒重复测定8次，分别计算不同浓度样本的平均值及SD值。
批内差： CV<10%
批间差： CV<12%
线性
在定值血清及血浆样本内加入适量的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)，并倍比稀释成1:2，1:4，1:8，1:16的待测样本，线性范围即为稀释后样本中中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。
| 样本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 83-98% | 78-97% | 87-94% | 79-89% |
| EDTA plasma(n=5) | 81-89% | 89-97% | 80-88% | 78-104% |
| heparin plasma(n=5) | 81-88% | 79-93% | 80-98% | 82-96% |
稳定性
经测定，试剂盒在有效期内按推荐温度保存，其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响，实验室的环境条件需尽量保持一致，尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。
实验流程
1. 实验前标准品、试剂及样本准备；
2. 加样（标准品、样本、磁珠）标准品或样本100μL及磁珠10μL，
37°C酶标板振荡器孵育90分钟；
3. 磁吸甩干，加检测溶液A100μL，37°C酶标板振荡器孵育60分钟；
4. 磁吸洗板3次；
5. 加检测溶液B100μL，37°C振动孵育30分钟；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋涡震荡2分钟后读数。
实验原理
将中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠，制成固相载体，向微孔中分别加入标准品或标本以及磁珠，其中的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体结合，然后加入生物素化的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)抗体，将未结合的生物素化抗体洗净后，加入PE标记的亲和素，再次彻底洗涤后即可上机读数。MFI值和样品中的中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法)呈正相关。
赠品
增值服务
相关产品
| 编号 | 适用物种：Canis familiaris; Canine (Dog，犬) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA181Ca01 | 中性粒细胞弹性蛋白酶(NE)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA181Ca01 | 中性粒细胞弹性蛋白酶(NE)多克隆抗体 | WB; IHC |
| MAA181Ca21 | 中性粒细胞弹性蛋白酶(NE)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA181Ca | 中性粒细胞弹性蛋白酶(NE)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA181Ca | 中性粒细胞弹性蛋白酶(NE)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
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