催产素(OT)等多因子检测试剂盒(流式荧光发光法)

Multiplex Assay Kit for Oxytocin (OT) ,etc. by FLIA (Flow Luminescence Immunoassay)

Pitocin; Syntocinon

(注:单次混测多因子不超过8个指标 )

  • 催产素(OT)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • 催产素(OT)等多因子检测试剂盒(流式荧光发光法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测催产素(OT)等多因子检测试剂盒(流式荧光发光法),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的催产素(OT)等多因子检测试剂盒(流式荧光发光法)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 79-101 82
EDTA plasma(n=5) 85-99 90
heparin plasma(n=5) 89-97 94
sodium citrate plasma(n=5) 79-98 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的催产素(OT)等多因子检测试剂盒(流式荧光发光法),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中催产素(OT)等多因子检测试剂盒(流式荧光发光法)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 80-101% 94-101% 92-103% 85-101%
EDTA plasma(n=5) 90-105% 81-96% 99-105% 78-105%
heparin plasma(n=5) 85-99% 82-103% 95-102% 78-98%
sodium citrate plasma(n=5) 94-102% 83-95% 80-93% 85-103%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本准备;
2. 加样(标准品、样本、磁珠、检测溶液A)标准品或样本50μL及磁珠10μL,加检测溶液A50μL,
    37°C酶标板振荡器孵育60分钟;
3. 磁吸洗板3次;
4. 加检测溶液B100μL,37°C振动孵育30分钟;
5. 磁吸洗板3次;
6. 加鞘液100μL,旋涡震荡2分钟后读数。

实验原理

将催产素(OT)等多因子检测试剂盒(流式荧光发光法)抗体包被于磁珠,制成固相载体,向微孔中分别加入标准品或标本以及磁珠、标记VEGFA,标记的催产素(OT)等多因子检测试剂盒(流式荧光发光法)和未标记的催产素(OT)等多因子检测试剂盒(流式荧光发光法)与连接于固相载体上的抗体竞争结合,然后将未结合物洗净后,加入PE标记的亲和素,再次彻底洗涤后即可上机读数。MFI值和样品中的催产素(OT)等多因子检测试剂盒(流式荧光发光法)呈负相关。

赠品

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPB052Ge11 催产素(OT)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPB052Ge21 催产素(OT)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAB052Ge01 催产素(OT)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
HEB052Ge 催产素(OT)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB052Ge 催产素(OT)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB052Ge 催产素(OT)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB052Ge11 催产素(OT)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Psychiatr Res.? Sex-specific associations between plasma oxytocin levels and schizotypal personality features in healthy individuals [Pubmed:24411593]
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study [Pubmed:27143518]
Progress in neuro-psychopharmacology & biological psychiatry Increased oxytocin levels among abstinent heroin addicts: Association with aggressiveness, psychiatric symptoms and perceived childhood neglect. [pubmed:28093220]
International Journal of Clinical and Experimental Medicine Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic … [files:ijcem0031358.pdf]
Fertility and Sterility Administration of atosiban in patients with endometriosis undergoing frozen–thawed embryo transfer: a prospective, randomized study [pubmed:27143518]
BMC Med. Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial. [pubmed:28209155]
Psychiatry Research Plasma oxytocin levels in major depressive and bipolar II disorders [pubmed:28865715]
BMC Medicine Intranasal oxytocin reduces provoked symptoms in female patients with posttraumatic stress disorder despite exerting sympathomimetic and positive chronotropic effects in a randomized controlled trial [10.1186:s12916-017-0801-0]
EUROPEAN ADDICTION RESEARCH The Association between the Level of Plasma Oxytocin and Craving among Former Heroin Users [Pubmed:29902803]
ASN Neuro . Involvement of Hyperpolarization-Activated Cyclic Nucleotide-Gated Channel 3 in Oxytocin Neuronal Activity in Lactating Rats With Pup Deprivation [Pubmed: 32962418]
European Journal of Integrative Medicine Effects of transcutaneous electrical nerve stimulation applied at different frequencies during labor on hormone levels, labor pain perception, and anxiety: a randomized … []
J. Pers. Med Predicting the Treatment Outcomes of Antidepressants Using a Deep Neural Network of Deep Learning in Drug-Naïve Major Depressive Patients []
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