趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Chemokine (C-X-C Motif) Ligand 2 (CXCL2)

GROb; SCYB2; MIP2; GRO2; MIP2a; MGSAb; CINC2a; HSF; Macrophage inflammatory protein 2-alpha; Hematopoietic synergistic factor; Growth Regulated Oncogene Beta

  • 趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEB603Mu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 91-101 94
EDTA plasma(n=5) 80-98 90
heparin plasma(n=5) 90-104 93

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 92-99% 94-101% 97-105%
EDTA plasma(n=5) 83-94% 95-105% 86-101% 93-105%
heparin plasma(n=5) 81-90% 79-102% 96-105% 84-101%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL,37°C温育1小时;
3. 吸弃,加检测溶液A25µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B25µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分钟;
8. 加终止液20µL,立即450nm读数。

实验原理

将趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合,然后加入生物素化的趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
RPB603Mu01 趋化因子配体2(CXCL2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
EPB603Mu51 趋化因子配体2(CXCL2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB603Mu02 趋化因子配体2(CXCL2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB603Mu07 趋化因子配体2(CXCL2)多克隆抗体 WB; IHC; ICC; IP.
PAB603Mu02 趋化因子配体2(CXCL2)多克隆抗体 WB; IHC; ICC; IP.
PAB603Mu01 趋化因子配体2(CXCL2)多克隆抗体 WB; IHC; ICC; IP.
PAB603Mu51 趋化因子配体2(CXCL2)多克隆抗体 WB; IHC; ICC; IP.
SEB603Mu 趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB603Mu 趋化因子配体2(CXCL2)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB603Mu 趋化因子配体2(CXCL2)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
FEBS Journal Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced chemokine release in both TRAIL-resistant and TRAIL-sensitive cells via nuclear factor kappa B [PubMed: 19120450]
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source]
Physiol Genomics. Identification of human exercise-induced myokines using secretome analysis [Pubmed:24520153]
Diabetes Protein Inhibitor of Activated STAT 1 (PIAS1) Protects Against Obesity-Induced Insulin Resistance by Inhibiting Inflammation Cascade in Adipose Tissue [PubMed: 26324179]
Respiration Bubble CPAP support after discontinuation of mechanical ventilation protects rat lungs with ventilator-induced lung injury [Pubmed:26800273]
Scientific Reports The Transcriptional Foundations of Sp110-mediated Macrophage (RAW264. 7) Resistance to Mycobacterium tuberculosis H37Ra [Pubmed:26912204]
Cellular Physiology and Biochemistry Dexmedetomidine Alleviates HyperoxiaInduced Acute Lung Injury via Inhibiting NLRP3 Inflammasome Activation [pubmed:28873369]
Medicine (Baltimore) Rapid detection of urinary soluble intercellular adhesion molecule-1 for determination of lupus nephritis activity [Pubmed:29953010]
Hepatology Integrated Omics Reveals Tollip as an Aggravator and Therapeutic Target for Hepatic Ischemia‐Reperfusion Injury in Mice [Pubmed: 31077413]
Molecular Medicine Reports IL‑17A promotes CXCR2‑dependent angiogenesis in a mouse model of liver cancer [Pubmed: 31173199]
Anticancer Research The Clinicopathological Significance of the CXCR2 Ligands, CXCL1, CXCL2, CXCL3, CXCL5, CXCL6, CXCL7, and CXCL8 in Gastric Cancer [Pubmed: 31810929]
Lab Invest Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis [Pubmed: 32709888]
SCIENCE OF THE TOTAL ENVIRONMENT Assessment of acute toxicological effects of molybdenum (IV) disulfide nano-and microparticles after single intratracheal administration in rats [Pubmed: 32629262]
Autophagy Vitamin D3 and carbamazepine protect against Clostridioides difficile infection in mice by restoring macrophage lysosome acidification [Pubmed:34989311]
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