透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Hyaluronic Acid (HA)

Hyaluronan; Hyaluronate

  • 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEA182Ge.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 90-103 97
EDTA plasma(n=5) 78-89 84
heparin plasma(n=5) 80-97 80

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 96-104% 80-92% 83-92% 79-98%
EDTA plasma(n=5) 98-105% 90-101% 97-105% 80-94%
heparin plasma(n=5) 82-93% 78-94% 90-104% 80-93%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL后,立即加入检测溶液A25µL,
    37°C孵育1小时;
3. 甩干,洗板3次;
4. 加检测溶液B50µL,37°C孵育30分钟;
5. 洗板5次;
6. 加TMB底物50µL,37°C孵育10-20分钟;
7. 加终止液25µL,立即450nm读数。

实验原理

本试剂盒应用竞争抑制酶联免疫分析法测定标本中待测物质水平。将透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本)单克隆抗体包被微孔板,制成固相载体,往包被抗体的微孔中同时加入生物素标记的抗原和待测抗原(标准品或样本),待测抗原与生物素标记抗原对特异性抗体进行竞争结合。温育后经洗涤去掉未结合物,然后加入HRP标记的亲和素,经过温育和彻底洗涤后加入底物TMB显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。待测标本浓度越高,标记抗原和抗体的结合就越受到抑制,显色愈浅。显色的深浅与酶量呈正相关,而与样品中待测物质含量呈负相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Pan-species (General,通用) 应用(仅供研究使用,不用于临床诊断!)
CPA182Ge11 透明质酸(HA)牛血清白蛋白偶联物 Immunogen; SDS-PAGE; WB.
CPA182Ge21 透明质酸(HA)卵白蛋白偶联物 Immunogen; SDS-PAGE; WB.
PAA182Ge01 透明质酸(HA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
PAA182Ge02 透明质酸(HA)多克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA182Ge81 透明质酸(HA)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA182Ge71 透明质酸(HA)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA182Ge22 透明质酸(HA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MAA182Ge21 透明质酸(HA)单克隆抗体 ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
MEA182Ge 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA182Ge 透明质酸(HA)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA182Ge 透明质酸(HA)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSA182Ge11 透明质酸(HA)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Clinical Infectious Diseases Hemorrhagic Fever Caused by a Novel Bunyavirus in China: Pathogenesis and Correlates of Fatal Outcome [OxfordJournals: source]
Critical Care Medicine Alveolar fluid in acute respiratory distress syndrome promotes fibroblast migration: Role of platelet-derived growth factor pathway* [PubMed: 22713216]
European Archives of Oto-Rhino-Laryngology Age effects on extracellular matrix production of vocal fold scar fibroblasts in rats [Pubmed: 24077847]
Journal of Voice Vocal Fold Fibroblast Response to Growth Factor Treatment is Age Dependent: Results From an In Vitro Study [ScienceDirect: S0892199713002415]
Gynecol Oncol. Resveratrol inhibits ovarian cancer cell adhesion to peritoneal mesothelium in vitro by modulating the production of α5β1 integrins and hyaluronic acid [Pubmed:24995580]
Laryngoscope. Establishing principles of macromolecular crowding for in vitro fibrosis research of the vocal fold lamina propria [Pubmed:25545625]
Life Sci Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis [PubMed: 25936962]
Nutr Cancer Camel Milk: Potential Utility as an Adjunctive Therapy to Peg-IFN/RBV in HCV-5 Infected Patients in Egypt [PubMed: 26492131]
Journal of Clinical Gastroenterology and Hepatology Study of Some Serum Biochemical Markers of Liver Fibrosis in Patients with Chronic HCV [ISSN 2575-7733]
BMC Complementary and Alternative Medicine A putative Chondroprotective role for IL-1β and MPO in herbal treatment of experimental osteoarthritis [pubmed:29166937]
Experimental and Therapeutic Medicine Polycomb group proteins: Novel molecules associated with ultraviolet A-induced photoaging of human skin. [pubmed:28962194]
European Journal of Applied Physiology The effect of aerobic walking and lower body resistance exercise on serum COMP and hyaluronan, in both males and females [Pubmed:29536174]
European Food Research and Technology Evaluation of antifibrotic effects of coffee and cocoa extracts in rats with thioacetamide-induced fibrosis [10.1007:s00217-018-3119-z]
BioMed Research International Juvenile Ovine Ex Vivo Larynges: Phonatory, Histologic, and Micro CT Based Anatomic Analyses []
Advances in Medical Sciences Selected elements of extracellular matrix of the skin in diabetes and insulin resistance [Pubmed: 31146169]
Viruses Strain-Dependent Porcine Circovirus Type 2 (PCV2) Entry and Replication in T-Lymphoblasts [Pubmed: 31480752]
Physiology International Betulin attenuated liver damage by prevention of hepatic mitochondrial dysfunction in rats with alcoholic steatohepatitis [Pubmed: 31619044]
Journal of Clinical Medicine Damage-Associated Molecular Patterns and Th-Cell-Related Cytokines Released after Progressive Effort [Pubmed: 32210109]
Journal of Proteomics iTRAQ-based analysis for the identification of MARCH8 targets in human esophageal squamous cell carcinoma [33540066]
Eur J Appl Physiol An exploratory study to investigate the association between age, physical activity, femoral trochlear cartilage thickness and biomarkers of tissue metabolism in?¡­ [33713200]
J Cell Physiol Trimethylamine N‐oxide exacerbates acetaminophen‐induced liver injury by interfering with macrophage‐mediated liver regeneration [34459512]
Experimental Cell Research Comparative studies on the effect of pig adipose-derived stem cells (pASCs) preconditioned with hypoxia or normoxia on skin wound healing in mice [Pubmed:35718003]
Biomedicine & Pharmacotherapy Bicyclol ameliorates advanced liver diseases in murine models via inhibiting the IL-6/STAT3 signaling pathway [Pubmed:35658240]
Sensors and Actuators B: Chemical Novel multifunctional delivery system for chondrocytes and articular cartilage based on carbon quantum dots []
留言咨询