核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Nuclear Factor Kappa B (NFkB)

NF-KB1; EBP-1; KBF1; NF-Kappa-B; NFKB-P105; NFKB-P50; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 1; Nuclear factor NF-kappa-B p105 subunit

  • 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEB824Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL,37°C温育1小时;
3. 吸弃,加检测溶液A25µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B25µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分钟;
8. 加终止液20µL,立即450nm读数。

实验原理

将核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合,然后加入生物素化的核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPB824Hu01 核因子κB(NFkB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB824Hu03 核因子κB(NFkB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB824Hu02 核因子κB(NFkB)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB824Hu01 核因子κB(NFkB)多克隆抗体 WB; IHC; ICC; IP.
PAB824Hu02 核因子κB(NFkB)多克隆抗体 WB; IHC; ICC; IP.
LAB824Hu81 核因子κB(NFkB)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAB824Hu71 核因子κB(NFkB)多克隆抗体(生物素标记) WB; IHC; ICC.
MAB824Hu22 核因子κB(NFkB)单克隆抗体 WB; IHC; ICC; IP.
SEB824Hu 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEB824Hu 核因子κB(NFkB)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB824Hu 核因子κB(NFkB)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.
KSB824Hu01 核因子κB(NFkB)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Reproductice Science Surgical Removal of Endometrioma Decreases the NF-kB1 (p50/105) and NF-kB p65 (Rel A) Expression in the Eutopic Endometrium During the Implantation Window [Sagepub: Source]
International Immunopharmacology Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis [PubMed: 21642017]
PLoS One New Method to Isolate and Culture Rat Kupffer Cells [PubMed: PMC3743898]
World Journal of Gastroenterology Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in rats [PubMed: PMC3660814]
Experimental and Therapeutic Medicine Shuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κB [PubMed: PMC3570159]
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
Free Radical Biology and Medicine Involvement of nitric oxide with activation of Toll-like receptor 4 signaling in mice with dextran sodium sulfate-induced colitis [Pubmed:24992835]
BBA Clin Long-term warfarin therapy and biomarkers for osteoporosis and atherosclerosis [PubMed: 26674156]
J Clin Diagn Res Serological Analysis of Alpha-synuclein and NF-κB in Parkinson’s Disease Patients [PubMed: 26155470]
Eur J Obstet Gynecol Reprod Biol Hysteroscopic polypectomy decreases NF-κB1 expression in the mid-secretory endometrium of women with endometrial polyp [PubMed: 25898371]
Asian Pacific Journal of Cancer Prevention Plasma nuclear factor kappa B and serum peroxiredoxin 3 in early diagnosis of hepatocellular carcinoma. [PubMed: 25743848]
Acta Biochim Pol Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis. [PubMed: 26345092]
Acta Veterinaria Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute Colitis [View: J]
Neurochem Res Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat [PubMed: 26700436]
Transplant Proc L-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients [PubMed: 26518955]
Journal of Food Science  Effects of Hyriopsis cumingii Polysaccharides on Mice Immunologic Receptor, Transcription Factor, and Cytokine [Pubmed:27061742]
IJBPAS THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS [publication:298838934]
Basic & Clinical Pharmacology & Toxicology Placental Growth Factor Triggers Epithelial-to-Mesenchymal Transition-like Changes in Rat Type II Alveolar Epithelial Cells: Activation of Nuclear Factor κB Signalling Pathway [doi:10.1111]
Peptides. Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms. [pubmed:27777064]
british journal of pharmacology Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats. [pubmed:27590029]
european journal of pharmacology Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms. [pubmed:27915041]
european journal of pharmacology S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase. [pubmed:27887948]
Psychopharmacology (Berl) Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms. [pubmed:28303372]
biomedicine & pharmacotherapy Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat. [pubmed:28199883]
biomedicine & pharmacotherapy Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation. [pubmed:28033582]
Toxicology and Applied Pharmacology Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454]
Microbial Immunology Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota [pubmed:28713364]
Journal of Biomechanics The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis [pubmed:29137729]
European Journal of Pharmacology In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesis [pubmed:29126792]
Frontiers in Pharmacology Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. [pubmed:28553224]
International Journal of Medicinal Mushrooms Anti-Inflammatory Activity and Bioactive Constituents of Cultivated Fruiting Bodies of Xylaria nigripes (Ascomycetes), a Chinese Medicinal Fungus. [pubmed:29256845]
Biomedicine & pharmacotherapy Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms [pubmed:28061403]
International Immunopharmacology Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms. [pubmed:29190543]
Food and Chemical Toxicology Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic rats [Pubmed:30026088]
Neurotoxicity Research Protective Effects of Fibroblast Growth Factor 21 Against Amyloid-Beta-Induced Toxicity in SH-SY5Y Cells [Pubmed:29869772]
European Journal of Pharmacology  Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat [Pubmed:29518393]
Experimental biology and medicine (Maywood) Shock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse model [Pubmed:29984607]
Acta Veterinaria Belgrade Anti-inflammatory potential of Lactobacillus plantarum LS/07 in acute colitis in rats [10.2478:acve-2018-0005]
Taiwanese Journal of Obstetrics & Gynecology HMGB1-RAGE signaling pathway in pPROM [Pubmed:29673663]
Acta Veterinaria Brno Improvement of colitis by LS/07 and inulin [10.2754:avb201786040399]
Drug Discoveries & Therapeutics The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4 [Pubmed:29760339]
Life Sciences Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic rats [Pubmed: 30205096]
Life Sciences Sitagliptin attenuates intestinal ischemia/reperfusion injury via cAMP/PKA, PI3K/Akt pathway in a glucagon-like peptide 1 receptor-dependent manner [Pubmed: 30195035]
Journal of Traditional Chinese Medical Sciences Effect of Dachaihu Decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet []
Naunyn-Schmiedeberg's archives of pharmacology Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2 [Pubmed: 30474696]
Archives of Physiology and Biochemistry Diabetes-induced testicular oxidative stress, inflammation, and caspase-dependent apoptosis: the protective role of metformin [Pubmed: 30513216]
Journal of Functional Foods Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments []
Scientific Reports Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis [Pubmed: 30651572]
Journal of Pain Researc Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain [Pubmed: 30881100]
Antioxidants Oxidative Stress, NF-κB-Mediated Inflammation and Apoptosis in the Testes of Streptozotocin–Induced Diabetic Rats: Combined Protective Effects of Malaysian … [Pubmed: 31600920]
journal of biochemical and molecular toxicology Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells [Pubmed: 31916655]
Life Sci New insights into the protection of growth hormone in cisplatin-induced nephrotoxicity: The impact of IGF-1 on the Keap1-Nrf2/HO-1 signaling [Pubmed: 32209424]
European Review for Medical and Pharmacological Sciences Effects of lncRNA gm4419 on rats with hypertensive cerebral atherosclerosis through NF-κB pathway [Pubmed: 31858567]
J Ethnopharmacol Effects of fermented ginseng on the gut microbiota and immunity of rats with antibiotic-associated diarrhea [Pubmed: 33217518]
Odontology Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337]
LIFE SCIENCES Effects of combined treatment with Indomethacin and Juglone on AOM/DSS induced colon carcinogenesis in Balb/c mice: Roles of inflammation and … [Pubmed: 33148421]
JOURNAL OF ETHNOPHARMACOLOGY Morroniside attenuates apoptosis and pyroptosis of chondrocytes and ameliorates osteoarthritic development by inhibiting NF-κB signaling [Pubmed: 33022338]
Journal of Cosmetic Dermatology Examination of androgenetic alopecia with serum biomarkers [Pubmed: 32969583]
NEUROPSYCHOBIOLOGY Diosgenin Attenuates Cognitive Impairment in Streptozotocin-Induced Diabetic Rats: Underlying Mechanisms [Pubmed: 32526752]
journal of ethnopharmacology UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in rats [Pubmed: 32376365]
BIOMEDICINE & PHARMACOTHERAPY Protective effects of bee bread on testicular oxidative stress, NF-κB-mediated inflammation, apoptosis and lactate transport decline in obese male rats [Pubmed: 33152939]
Journal of Diabetes Research Regulation and Mechanism of miR-518d through the PPARα-Mediated NF-κB Pathway in the Development of Gestational Diabetes Mellitus [Pubmed: 33123597]
Folia Biologica Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats []
TOXICOLOGY IN VITRO Cyproheptadine causes apoptosis and decreases inflammation by disrupting thiol/disulfide balance and enhancing the levels of SIRT1 in C6 glioblastoma cells [33675893]
Hum Exp Toxicol Alamandine significantly reduces doxorubicin-induced cardiotoxicity in rats [33882726]
J Food Biochem Masseter muscle and gingival tissue inflammatory response following treatment with high©\fructose corn syrup in rats: Anti©\inflammatory and antioxidant effects of kefir [33864286]
Open Access Macedonian Journal of Medical Sciences The Potential of Nano Curcumin in Preventing the Formation of Artificial Antisperm Antibody in Wistar Rats through Inflammatory Pathway Regulation []
Eur Rev Med Pharmacol Sci Vitamin D supplementation inhibits NF-kß signaling pathway in lean and obese women with PCOS [Pubmed:35731068]
BMC Nephrol Effect of subacute poisoning with lambdacyhalothrin on vascular endothelial growth factor 2 receptor in mice kidneys [Pubmed:35643467]
Antioxidants Therapeutic Effects of Bee Bread on Obesity-Induced Testicular-Derived Oxidative Stress, Inflammation, and Apoptosis in High-Fat Diet Obese Rat Model … [Pubmed:35204140]
Journal of Pharmaceutical Research International Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid … []
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