骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)

Mini Samples ELISA Kit for Osteoprotegerin (OPG)

TNFRSF11B; TNFRSF11-B; OCIF; OPG; TR1; Tumor Necrosis Factor Receptor Superfamily Member 11b; Osteoclastogenesis Inhibitory Factor

  • 骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本) 产品包装(模拟)
  • 骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本) 实验结果图
  • MEA108Hu.jpg 标准曲线图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

本试剂盒用于检测骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本),经检测与其它相似物质无明显交叉反应。
由于受到技术及样本来源的限制,不可能完成对所有相关或相似物质交叉反应检测,因此本试剂盒有可能与未经检测的其它物质有交叉反应。

回收率

分别于定值血清及血浆样本中加入一定量的骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)(加标样品),重复测定并计算其均值,回收率为测定值与理论值的比率。

样本 回收率范围(%) 平均回收率(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 83-99 88
heparin plasma(n=5) 82-104 101

精密度

精密度用样品测定值的变异系数CV表示。CV(%) = SD/mean×100
批内差:取同批次试剂盒对低、中、高值定值样本进行定量检测,每份样本连续测定20 次,分别计算不同浓度样本的平均值及SD值。
批间差:选取3个不同批次的试剂盒分别对低、中、高值定值样本进行定量测定,每个样本使用同一试剂盒重复测定8次,分别计算不同浓度样本的平均值及SD值。
批内差: CV<10%
批间差: CV<12%

线性

在定值血清及血浆样本内加入适量的骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本),并倍比稀释成1:2,1:4,1:8,1:16的待测样本,线性范围即为稀释后样本中骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)含量的测定值与理论值的比率。

样本 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 84-91% 79-101% 94-105%
EDTA plasma(n=5) 78-91% 78-99% 92-99% 80-99%
heparin plasma(n=5) 87-101% 93-101% 97-104% 83-97%

稳定性

经测定,试剂盒在有效期内按推荐温度保存,其活性降低率小于5%。
为减小外部因素对试剂盒破坏前后检测值的影响,实验室的环境条件需尽量保持一致,尤其是实验室内温度、湿度及温育条件。其次由同一实验员来进行操作可减少人为误差。

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)25µL,37°C温育1小时;
3. 吸弃,加检测溶液A25µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B25µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物25µL,37°C孵育10-20分钟;
8. 加终止液20µL,立即450nm读数。

实验原理

将骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)与连接于固相载体上的抗体结合,然后加入生物素化的骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
EPA108Hu61 骨保护素(OPG)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA108Hu61 骨保护素(OPG)活性蛋白 Cell culture; Activity Assays.
RPA108Hu01 骨保护素(OPG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA108Hu02 骨保护素(OPG)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA108Hu01 骨保护素(OPG)活性蛋白 Cell culture; Activity Assays.
PAA108Hu06 骨保护素(OPG)多克隆抗体 WB; IHC; ICC; IP.
PAA108Hu01 骨保护素(OPG)多克隆抗体 WB; IHC; ICC; IP.
MAA108Hu23 骨保护素(OPG)单克隆抗体 WB; IHC; ICC; IP.
MAA108Hu22 骨保护素(OPG)单克隆抗体 WB; IHC; ICC; IP.
MAA108Hu21 骨保护素(OPG)单克隆抗体 WB; IHC; ICC; IP.
SEA108Hu 骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA108Hu 骨保护素(OPG)检测试剂盒(酶联免疫吸附试验法,小样本) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA108Hu 骨保护素(OPG)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA108Hu 骨保护素(OPG)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Pharmacology Protective Effect of Amlodipine on Rat Bone Tissue after Orchidectomy [Karger: 335491]
Endocrine Pharmacology Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar rats [ScienceDirect: S0014299912000659]
PLOS One Adipokines in Psoriatic Arthritis Patients: The Correlations with Osteoclast Precursors and Bone Erosions [PubMed: PMC3483160]
International Journal of Oral and Maxillofacial Surgery The effects of chronic zoledronate usage on the jaw and long bones evaluated using RANKL and osteoprotegerin levels in an animal model [Pubmed: 23522850]
American Journal of Orthodontics and Dentofacial Orthopedics OPG and RANKL levels around miniscrew implants during orthodontic tooth movement [Pubmed: 23910201]
AGE Estrogen deficiency does not decrease the in vitro osteogenic potential of rat adipose-derived mesenchymal stem cells [Pubmed: 24687841]
Food Science & Nutrition Effects and mechanisms of 8-prenylnaringenin on osteoblast MC3T3-E1 and osteoclast-like cells RAW264.7 [Onlinelibrary: fsn3.109]
Food Sci Nutr. Effects and mechanisms of 8‐prenylnaringenin on osteoblast MC3T3‐E1 and osteoclast‐like cells RAW264. 7 [Pubmed:Pmc4221832]
J Neurol Sci. The effect of topiramate and lamotrigine on rat bone mass, structure and metabolism. [Pubmed:24629477]
Journal of Investigative Surgery Effect of Nicotine on RANKL and OPG and Bone Mineral Density [Pubmed:24830656]
Int J Mol Med. Effects of icariin on the regulation of the OPG?RANKL?RANK system are mediated through the MAPK pathways in IL?1β?stimulated human SW1353 chondrosarcoma … [Pubmed:25270538]
Clinical and Experimental Pharmacology and Physiology Carvedilol promotes neurological function, reduces bone loss and attenuates cell damage after acute spinal cord injury in rats [Pubmed:25424914]
Journal of Colloid and Interface Science Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198]
Int J Clin Exp Pathol A novel model of bisphosphonate-related osteonecrosis of the jaw in rats [PubMed: 26191212]
IJHS(international journal of health sciences) Relation of Osteoprotegerin, Visfatin and Ghrelin to Metabolic Syndrome in Type 2 Diabetic Patients [PubMed: 26309431]
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861]
Experimental And Therapeutic Medicine Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway [Pubmed:27347050]
Bone The effects of strength training and raloxifene on bone health in aging ovariectomized rats [Pubmed:26812611]
Scienze Biomediche Effetti del laser a bassa dose su cellule osteoblastiche [INCERTIPARENTI_SERENA_TESI.pdf]
journal of biomedical materials research part a RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis. [pubmed:27667508]
Nutrition. Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats. [pubmed:27908549]
PLoS One. Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228]
Sci Rep. Skeletal Site-specific Effects of Zoledronate on in vivo Bone Remodeling and in vitro BMSCs Osteogenic Activity. [pubmed:28139685]
Journal of Biomaterials and Tissue Engineering Modification and Characterization of Polyethylene Glycolated Decellularized Porcine Aortic Heart Valve with Osteoprotegerin [art00007]
Japanese Society for Bone and Mineral Research Allogeneic yet major histocompatibility complex‑matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced trabecular bone [article:10.1007]
ONCOLOGY LETTERS Assessment of bone metabolism and biomechanical properties of the femur, following treatment with anastrozole and letrozole in an experimental model of menopause [ol.2017.6596]
Journal of Biomedical Materials Research RAW 264.7 co-cultured with ultra-high molecular weight polyethylene particles spontaneously differentiate into osteoclasts: an in vitro model of periprosthetic osteolysis [10.1002/jbm.a.35912]
Oncotarget Mesenchymal stem cells inhibit RANK-RANKL interactions between osteoclasts and Th17 cells via osteoprotegerin activity. [pubmed:29137353]
Asian Journal of Biomedical and Pharmaceutical Sciences A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/β-catenin Signaling Pathways in Ovariectomized Rats [ISSN 2249 – 622X]
ACS Applied Materials and Interfaces Enhanced Osseointegration of Porous Titanium Modified with Zeolitic Imidazolate Framework-8. [pubmed:28696091]
Journal of Clinical Periodontology  Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908]
Journal of Functional Foods Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats [10.1016:j.jff.2018.04.048]
Macromolecular Bioscience Strontium‐Substituted Hydroxyapatite‐Gelatin Biomimetic Scaffolds Modulate Bone Cell Response [Pubmed:29877029]
Journal of Ethnopharmacology Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model [Pubmed:29705515]
International Journal of Molecular Medicine Bu‑Shen‑Ning‑Xin decoction suppresses osteoclastogenesis by modulating RANKL/OPG imbalance in the CD4+ T lymphocytes of ovariectomized mice [Pubmed:29717766]
Journal of Bone and Mineral Metabolism Allogeneic yet major histocompatibility complex-matched bone marrow transplantation in mice results in an impairment of osteoblasts and a significantly reduced … [Pubmed:28766138]
Journal of Bone and Mineral Metabolism  Therapeutic effect of icariin combined with stem cells on postmenopausal osteoporosis in rats [Pubmed:28681147]
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457]
Experimental and Therapeutic Medicine Liraglutide exerts a bone‑protective effect in ovariectomized rats with streptozotocin‑induced diabetes by inhibiting osteoclastogenesis [Pubmed:29805533]
Bone The sole and combined effect of simvastatin and platelet rich fibrin as a filling material in induced bone defect in tibia of albino rats [Pubmed: 30208342]
Bone Research NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992]
Bone Development and characterization of supramolecular calcitonin assembly and assessment of its interactions with the bone remodelling process [Pubmed: 30797058]
RSC Advances Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcification [Doi: 10.1039/C9RA00408D]
ANTICANCER RESEARCH Promoter Methylation Down-regulates Osteoprotegerin Expression in Ovarian Carcinoma [Pubmed: 31092428]
BIOSCIENCE REPORTS IL-38 restrains inflammatory response of collagen-induced arthritis in rats via SIRT1/HIF-1α signaling pathway [Pubmed: 32347300]
Oxid Med Cell Longev Long-Term Administration of Abacavir and Etravirine Impairs Semen Quality and Alters Redox System and Bone Metabolism in Growing Male Wistar Rats [34373766]
Biomed Pharmacother Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis [Pubmed:35453007]
Medicina (Kaunas) Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980]
Journal of Orthopaedic Translation Exercised accelerated the production of muscle-derived kynurenic acid in skeletal muscle and alleviated the postmenopausal osteoporosis through the Gpr35 … []
留言咨询