肿瘤蛋白p53(P53)单克隆抗体
Monoclonal Antibody to Tumor Protein p53 (P53)
TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
- 编号MAA928Ra22
- 物种Rattus norvegicus (Rat，大鼠) 相同的名称，不同的物种。
- 来源单抗制备
- 宿主小鼠
- 效价(克隆号)D10
- Ig亚型 IgG2a lambda
- 纯化方式蛋白A + 蛋白G亲和纯化
- 标记物无标记物
- 免疫原 RPA928Ra01-肿瘤蛋白p53(P53)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度0.45mg/ml
- 且适物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，猪), Bos taurus; Bovine (Cattle，牛), Capra hircus; Caprine (Goat，山羊), Chicken (Gallus，鸡)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 659 ¥ 1537 ¥ 2196 ¥ 5490 ¥ 21960
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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FITC staining on IF; Sample: Human HepG2 cell; Primary Ab: 40μg/ml Mouse Anti-Rat TP53 Antibody Second Ab: 5μg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu18)
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Western Blot; Sample: Lane1: Rat Colon lysate; Lane2: A431 cell lysate Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Porcine Colon lysate; Lane2: Porcine Spleen lysate; Lane3: Porcine Mammary gland lysate; Lane4: Porcine Skin lysate
Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: 3T3-L1 cell lysate; Lane2: RAW264.7 cell lysate; Lane3: Canine Colon lysate; Lane4: Gallus Small intestine lysate; Lane5: Bovine Colon lysate; Lane6: Caprine Colon lysate
Primary Ab: 0.5ug/ml Mouse Anti-Rat P53 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对P53的小鼠单克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别P53。
用法
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-40µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Rattus norvegicus (Rat，大鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPA928Ra01 | 肿瘤蛋白p53(P53)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA928Ra01 | 肿瘤蛋白p53(P53)活性蛋白 | Cell culture; Activity Assays. |
| PAA928Ra01 | 肿瘤蛋白p53(P53)多克隆抗体 | WB,ICC/IF |
| MAA928Ra22 | 肿瘤蛋白p53(P53)单克隆抗体 | WB; IHC; ICC; IP. |
| MAA928Ra21 | 肿瘤蛋白p53(P53)单克隆抗体 | WB; IHC; ICC; IP. |
| SEA928Ra | 肿瘤蛋白p53(P53)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA928Ra | 肿瘤蛋白p53(P53)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Anticancer Research | Analysis of p53 and miRNA expression after irradiation of glioblastoma cell lines [PubMed: 23155233] |
| Microscopy Research and Technique | Therapeutic role of curcumin in oxidative DNA damage caused by formaldehyde [PubMed: 25761397] |
| J Nutr Biochem | Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing mice [PubMed: 26344777] |
| Biochemical and Biophysical Research Communications | Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytes [Pubmed: 31331641] |
| Folia Biologica | Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats [] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β … [] |
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