CD73分子(CD73)多克隆抗体
Polyclonal Antibody to Cluster Of Differentiation 73 (CD73)
NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto
- 编号PAB250Bo01
- 物种Bos taurus; Bovine (Cattle，牛) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPB250Bo01-CD73分子(CD73)重组蛋白
- 缓冲液成份磷酸盐缓冲液（pH7.4,含有0.02% NaN3和50%甘油）
- 性状液体
- 浓度500µg/mL
- 且适物种Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 716 ¥ 1672 ¥ 2388 ¥ 5970 ¥ 23880
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Figure. Western Blot; Sample: Recombinant NT5E, Bovine.
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Western Blot; Sample: Bovine Cerebrum lysate;
Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type K562 cell lysate;
Lane 2: NT5E knockout K562 cell lysate;
Predicted MW: 63kd
Observed MW: 70kd
Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Bovine Liver lysate;
Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human K562 cell lysate;
Primary Ab: 3µg/ml Rabbit Anti-Bovine NT5E Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对CD73的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别CD73。
用法
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Immunocytochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Bos taurus; Bovine (Cattle，牛) | 应用(仅供研究使用，不用于临床诊断！) |
| RPB250Bo01 | CD73分子(CD73)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB250Bo01 | CD73分子(CD73)多克隆抗体 | WB; IHC; ICC; IP. |
参考文献
| 杂志 | 参考文献 |
| Journal of Autoimmunity | The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammation [Pubmed:25640206] |
| Talanta | Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment … [10.1016:j.talanta.2018.01.044] |
| Cancer Biomarkers | Expression and clinical significance of serum NT5E protein in patients with colorectal cancer [Pubmed: 30932882] |
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