Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体
Polyclonal Antibody to Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1)
EAT; MCL1L; MCL1S; BCL2L3; TM; Myeloid Cell Leukemia Sequence 1, Bcl2 Related; Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; mcl1/EAT
- 编号PAC615Mu01
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 来源多抗制备
- 宿主兔
- 效价-
- Ig类型 IgG
- 纯化方式抗原特异性亲和纯化
- 标记物无标记物
- 免疫原 RPC615Mu01-Bcl2相关髓细胞白血病序列1(MCL1)重组蛋白
- 缓冲液成份0.01M 磷酸盐缓冲液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性状液体
- 浓度1mg/ml
- 且适物种Homo sapiens (Human，人)
- 应用WB; IHC; ICC; IP.
如果抗体需用于流式细胞术，请参见流式抗体。 - 下载 英文说明书 中文说明书
- 规格 20µl100µl 200µl 1ml 10ml
- 价格 ¥ 648 ¥ 1512 ¥ 2160 ¥ 5400 ¥ 21600
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产品包装(模拟)
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产品包装(模拟)
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DAB staining on IHC-P; Samples: Mouse Intestine Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse MCL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Thyroid Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Lymphoma Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human Hela cell;
Primary Ab: 40μg/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant MCL1, Mouse.
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Western Blot; Sample: Mouse Spleen lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Small intestine lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Mouse MCL1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-Mouse MCL1 Polyclonal Antibody (Catalog PAC615Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通过ISO 9001、ISO 13485质量体系认证
特异性
该抗体是针对MCL1的兔多克隆抗体。在免疫组织化学染色和免疫印迹实验中能识别MCL1。
用法
Western blotting: 0.5-2µg/mL;1:500-2000
Immunohistochemistry: 5-20µg/mL;1:50-200
Immunocytochemistry: 5-20µg/mL;1:50-200
Optimal working dilutions must be determined by end user.
储存
经常使用则4°C保存。-20°C保存不超过两年。避免反复冻融。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
赠品
增值服务
相关产品
| 编号 | 适用物种：Mus musculus (Mouse，小鼠) | 应用(仅供研究使用，不用于临床诊断！) |
| RPC615Mu01 | Bcl2相关髓细胞白血病序列1(MCL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC615Mu02 | Bcl2相关髓细胞白血病序列1(MCL1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPC615Mu61 | Bcl2相关髓细胞白血病序列1(MCL1)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC615Mu01 | Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体 | WB; IHC; ICC; IP. |
| PAC615Mu02 | Bcl2相关髓细胞白血病序列1(MCL1)多克隆抗体 | WB; IHC; ICC; IP. |
| SEC615Mu | Bcl2相关髓细胞白血病序列1(MCL1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC615Mu | Bcl2相关髓细胞白血病序列1(MCL1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
参考文献
| 杂志 | 参考文献 |
| Cancers | Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia [Pubmed: 31718075] |
| ACTA NEUROPATHOLOGICA COMMUNICATIONS | Restoration of miR-193a expression is tumor-suppressive in MYC amplified Group 3 medulloblastoma [Pubmed: 32410663] |
| Cancers (Basel) | BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 [33540760] |
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