沉默调节蛋白1(SIRT1)重组蛋白
Recombinant Sirtuin 1 (SIRT1)
SIR2L1; Silent Mating Type Information Regulation 2 Homolog 1; NAD-Dependent Deacetylase Sirtuin-1; SIR2-like protein 1; SirtT1 75 kDa fragment
- 编号RPE912Hu01
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 来源原核表达
- 宿主E.coli
- 内毒素水平<1.0EU/µg(LAL法测定)
- 亚细胞定位细胞核
- 预测分子量30.3kDa
- 实际分子量19&28kDa(差异分析请参阅说明书)
- 片段与标签Glu244~Ala498 with N-terminal His Tag
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性状冻干粉
- 纯度> 95%
- 等电点5.9
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应用
Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，请参见活性蛋白。 - 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg
- 价格 ¥ 1200 ¥ 3000 ¥ 6000 ¥ 18000 ¥ 45000
- 欲了解实际交易价格和更多情况，请与当地经销商联系!
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产品包装(模拟)
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产品包装(模拟)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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通过ISO 9001、ISO 13485质量体系认证
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
储存
避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。
稳定性
热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。
增值服务
相关产品
| 编号 | 适用物种：Homo sapiens (Human，人) | 应用(仅供研究使用，不用于临床诊断！) |
| RPE912Hu01 | 沉默调节蛋白1(SIRT1)重组蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE912Hu01 | 沉默调节蛋白1(SIRT1)多克隆抗体 | WB; IHC; ICC; IP. |
| LAE912Hu81 | 沉默调节蛋白1(SIRT1)多克隆抗体(异硫氰酸荧光素标记) | WB; IHC; ICC; IF. |
| LAE912Hu71 | 沉默调节蛋白1(SIRT1)多克隆抗体(生物素标记) | WB; IHC; ICC. |
| MAE912Hu22 | 沉默调节蛋白1(SIRT1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAE912Hu23 | 沉默调节蛋白1(SIRT1)单克隆抗体 | WB; IHC; ICC; IP. |
| MAE912Hu21 | 沉默调节蛋白1(SIRT1)单克隆抗体 | WB; IHC; ICC; IP. |
| SEE912Hu | 沉默调节蛋白1(SIRT1)检测试剂盒(酶联免疫吸附试验法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME912Hu | 沉默调节蛋白1(SIRT1)等多因子检测试剂盒(流式荧光发光法) | FLIA Kit for Antigen Detection. |
| PSE912Hu01 | 沉默调节蛋白1(SIRT1)抗体对 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSE912Hu01 | 沉默调节蛋白1(SIRT1)检测试剂盒DIY材料(酶联免疫吸附试验法) | Main materials for "Do It (ELISA Kit) Yourself". |
参考文献
| 杂志 | 参考文献 |
| PLoS One. | SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases [Plos: Source] |
| PLOS ONE | A Novel Role for SIRT-1 in L-Arginine Protection against STZ Induced Myocardial Fibrosis in Rats [Pubmed:25501750] |
| Journal of Alzheimer's Disease | Serum miR-206 and miR-132 as Potential Circulating Biomarkers for Mild Cognitive Impairment [Pubmed:25589731] |
| Respirology | Increases in peripheral SIRT1: A new biological characteristic of asthma [PubMed: 26040995] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999] |
| Endocrine | Changes of serum Mir-217 and the correlation with the severity in type 2 diabetes patients with different stages of diabetic kidney disease. [pubmed:27522360] |
| Naunyn Schmiedebergs Arch Pharmacol | The potential curative effect of rebamipide in hepatic ischemia/reperfusion injury [pubmed:28361247] |
| International journal of cardiology | A sirtuin 1/MMP2 prognostic index for myocardial infarction in patients with advanced coronary artery disease [pubmed:28043667] |
| International Journal of Cardiology | Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial [pubmed:28029409] |
| Hepatology International | Association of SIRT1 gene polymorphism and its expression for the risk of alcoholic fatty liver disease in the Han population [pubmed:29189974] |
| Toxicology and Applied Pharmacology | Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1) [pubmed:28974454] |
| Chemico-biological Interactions | Magnolol protects pancreatic β-cells against methylglyoxal-induced cellular dysfunction [pubmed:28919305] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after … [Pubmed:30037068] |
| Experimental Gerontology | Specific alterations in the circulating levels of the SIRT1, TLR4, and IL7 proteins in patients with dementia [Pubmed:30071285] |
| Kidney and Blood Pressure Research | The Kidney Specific Protein myo-Inositol Oxygenase, a Potential Biomarker for Diabetic Nephropathy [Pubmed: 30504713] |
| Journal of International Medical Research | Serum and follicular fluid levels of sirtuin 1, sirtuin 6, and resveratrol in women undergoing fertilization: an observational, clinical study [Pubmed: 30556451] |
| BioMed Research International | Centella asiatica Prevents Increase of Hippocampal Tumor Necrosis Factor-Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of Chronic … [] |
| 学位论文 | Influência do tratamento da doença periodontal na sirtuína 1 e na lectina ligadora de manose em indivíduos com doença arterial coronária [] |
| Journal of Physics D: Applied Physics | Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility [] |
| Journal of Geriatric Oncology | Serum biomarkers for predicting overall survival and early mortality in older patients with metastatic solid tumors [Pubmed: 30952517] |
| Biomed Research International | Prevents Increase of Hippocampal Tumor Necrosis Factor-α Independently of Its Effect on Brain-Derived Neurotrophic Factor in Rat Model of … [Pubmed: 30956976] |
| Journal of Diabetes and its Complications | Vasohibin-1 in T2DM with different UACR [] |
| Clinical Interventions in Aging | The Serum Concentration of Anti-Aging Proteins, Sirtuin1 and αKlotho in Patients with End-Stage Kidney Disease on Maintenance Hemodialysis [Pubmed: 32214805] |
| Compr Psychiatry | Crocin alleviates schizophrenia-like symptoms in rats by upregulating silent information regulator-1 and brain derived neurotrophic factor [Pubmed: 33045669] |
| Сибирский федеральный университет | Влияние гречневой крупы на сиртуиновую активность у подопытных животных в эксперименте [] |
| Journal of Cereal Science | Dietary buckwheat enhances sirtuin1 without calorie restriction [] |
| Clin Interv Aging | Molecular Changes in Circulating microRNAs' Expression and Oxidative Stress in Adults with Mild Cognitive Impairment: A Biochemical and Molecular Study [33447019] |
| Antioxidants | The Effect of Repeated Whole-Body Cryotherapy on Sirt1 and Sirt3 Concentrations and Oxidative Status in Older and Young Men Performing Different Levels of?¡ [33396247] |
| Int Urol Nephrol | Evaluation of sirtuin 1 (SIRT1) levels in autosomal dominant polycystic kidney disease [33864594] |
| Neurochem Int | Dimethyl fumarate abridged tauo-/amyloidopathy in a D-Galactose/ovariectomy-induced Alzheimer's-like disease: Modulation of AMPK/SIRT-1, AKT/CREB/BDNF, AKT ¡ [34052296] |
| Toxicol Appl Pharmacol | Prasugrel anti-ischemic effect in rats: Modulation of hippocampal SUMO2/3-IкBα/Ubc9 and SIRT-1/miR-22 trajectories [34174262] |
| Food Chem | Buckwheat-enriched diet alleviates bisphenol A mediated oxidative stress via modulation of sirtuin 1 and antioxidant status in experimental rats [34763933] |
| Life Sci | Novel mechanistic insights towards the repositioning of alogliptin in Parkinson's disease [34774622] |
| Int J Mol Sci | Intralesional Infiltrations of Arteriosclerotic Tissue Cells-Free Filtrate Reproduce Vascular Pathology in Healthy Recipient Rats [Pubmed:35163435] |
| J Clin Med | Effects of Coffee on Sirtuin-1, Homocysteine, and Cholesterol of Healthy Adults: Does the Coffee Powder Matter? [Pubmed:35683374] |
| Cell & Bioscience | OGT Protects Skeletal Muscle From Damage Through SIRT1 O-GlcNAcylation During Cold Stress [] |
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