碱性磷酸酶(ALP)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Alkaline Phosphatase (ALP)

AKP; ALKP; Basic Phosphatase

  • 碱性磷酸酶(ALP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 碱性磷酸酶(ALP)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对碱性磷酸酶(ALP)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
PAB472Bo01 碱性磷酸酶(ALP)多克隆抗体 WB; IHC; ICC; IP.
LAB472Bo71 碱性磷酸酶(ALP)多克隆抗体(生物素标记) WB; IHC; ICC.
SEB472Bo 碱性磷酸酶(ALP)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB472Bo 碱性磷酸酶(ALP)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSB472Bo01 碱性磷酸酶(ALP)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Journal of Colloid and Interface Science Strontium and zoledronate hydroxyapatites graded composite coatings for bone prostheses [Pubmed:25706198]
Bone The active role of osteoporosis in the interaction between osteoblasts and bone metastases [PubMed: 26057367]
Macromolecular Bioscience Multi‐Layered Scaffolds for Osteochondral Tissue Engineering: In Vitro Response of Co‐Cultured Human Mesenchymal Stem Cells [PubMed: 26126665]
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Colloids Surf B Biointerfaces Antiresorption implant coatings based on calcium alendronate and octacalcium phosphate deposited by matrix assisted pulsed laser evaporation [PubMed: 26445021]
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PLoS One. Extracorporeal shock waves alone or combined with raloxifene promote bone formation and suppress resorption in ovariectomized rats. [pubmed:28158228]
Scientific Reports Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury throughAMP-activated protein kinase pathway. [pubmed:27678302]
J Cell Physiol. An advanced tri-culture model to evaluate the dynamic interplay among osteoblasts, osteoclasts, and endothelial cells. [pubmed:28240358]
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