铁蛋白(FE)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Ferritin (FE)

  • 铁蛋白(FE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 铁蛋白(FE)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对铁蛋白(FE)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

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KSA518Hu01 铁蛋白(FE)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Molecular Endocrinology PGC-1α regulates hepatic hepcidin expression and iron homeostasis in response to inflammation. [Pubmed: 23438894]
experimental oncology Redox-regulation of gelatinases during growth of cisplatin-sensitive and resistant Guerin carcinoma. [PubMed: 25804229]
J Cancer Sci Ther Host H68D Genotype Affects Tumor Growth in Mouse Melanoma [Open-Access: 1948-5956-1000353]
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Physiological research The Labile Iron Pool in Monocytes Reflects the Activity of the Atherosclerotic Process in Men with Chronic Cardiovascular Disease [pubmed:27782743]
Expert Review of Hematology 4 (th) Mediterranean Multidisciplinary Course on Iron Anemia April 29 (th)-30 (th) 2016, Madrid, Spain. [pubmed:27615544]
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
European Journal of Pharmaceutical Sciences sucrosomial® iron absorption studied by in vitro and ex-vivo models [pubmed:29055735]
Journal of Food Science and Technology In vitro assessment of bio-augmented minerals from peanut oil cakes fermented by Aspergillus oryzae through Caco-2 cells. [pubmed:29051659]
International Journal of Cuban Health & Medicine Anemia and Iron Deficiency Related to Inflammation, Helicobacter pylori Infection and Adiposity in Reproductive-age Cuban Women [10.1590/MEDICC.2017.1902030004 ]
Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599]
Food Bioscience Preparation of lactose-iron complex and its cyto-toxicity, in-vitro digestion and bioaccessibility in Caco-2 cell model system [10.1016/j.fbio.2017.10.001]
Nutrients. Long-Term Dexamethasone Exposure Down-Regulates Hepatic TFR1 and Reduces Liver Iron Concentration in Rats [pubmed:28629118]
Food Chemistry Physicochemical characterization of mineral (iron/zinc) bound caseinate and their mineral uptake in Caco-2 cells [Pubmed:29622185]
Animal Feed Science and Technology Super-dosing phytase improves the growth performance of weaner pigs fed a low iron diet [10.1016:j.anifeedsci.2018.01.012]
American Journal of Hematology Iron homeostasis in pregnancy and spontaneous abortion [Pubmed: 30394565]
The Nigerian postgraduate medical journal the official publication of the National Postgraduate Medical College of Nigeria Assessment of iron deficiency anaemia and its risk factors among adults with chronic kidney disease in a tertiary hospital in Nigeria []
Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208]
博士论文 PHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE []
Synthesis, characterization and cellular mineral absorption of nanoemulsions of Rhododendron arboreum flower extracts stabilized with gum arabic []
Cell Research Transferrin plays a central role in coagulation balance by interacting with clotting factors [Pubmed: 31811276]
international journal of biological macromolecules Gum arabic capped copper nanoparticles: Synthesis, characterization, and applications [Pubmed: 31904465]
journal of the american college of nutrition Improvement of Mineral Absorption and Nutritional Properties of Citrullus vulgaris Seeds Using Solid-State Fermentation [Pubmed: 32255407]
LWT-FOOD SCIENCE AND TECHNOLOGY Whey protein-iron or zinc complexation decreases pro-oxidant activity of iron and increases iron and zinc bioavailability []
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233]
European Journal of Pharmacology The mechanism of the imbalance between proliferation and ferroptosis in pulmonary artery smooth muscle cells based on the activation of SLC7A11 [Pubmed:35700835]
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