白介素18(IL18)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Interleukin 18 (IL18)

IGIF; IL-1g; IL1F4; IL1-F4; Interferon-Gamma-Inducing Factor; Interleukin-1 Family Member 4; Iboctadekin; Interleukin-1 Gamma

  • 白介素18(IL18)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 白介素18(IL18)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对白介素18(IL18)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
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SEA064Hu 白介素18(IL18)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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KSA064Hu01 白介素18(IL18)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
International Immunopharmacology Curcumin immune-mediated and anti-apoptotic mechanisms protect against renal ischemia/reperfusion and distant organ induced injuries [ScienceDirect: S1567576911001068]
Atherosclerosis IL-18 overexpression promotes vascular inflammation and remodeling in a rat model of metabolic syndrome [ScienceDirect: S0021915009006200]
PLoS One. HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa Keratitis [Plos: Source]
临床儿科杂志 中性粒细胞明胶酶相关脂质运载蛋白、肾损伤分子1和白介素18 在诊断心肺分流术后急性肾损伤中的意义 [Jcp-Sh:Source]
Cell Death Dis. MicroRNA-30d regulates cardiomyocyte pyroptosis by directly targeting foxo3a in diabetic cardiomyopathy [Pubmed:Pmc4237254]
PLoS One. Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion [Pubmed:24816434]
J Korean Med Sci. Acute Kidney Injury after Using Contrast during Cardiac Catheterization in Children with Heart Disease [Pubmed:25120320]
PLOS ONE Protective Effect of Leaf Essential Oil from [PubMed: 25794175]
Fertil Steril Inflammosome in the human endometrium: further step in the evaluation of the “maternal side” [PubMed: 26474737]
United States Patent Application GLUCAN-ENCAPSULATED SIRNA FOR TREATING TYPE 2 DIABETES MELLITUS [y2016:0138028.html]
Front Pharmacol Poria Attenuates Idiosyncratic Liver Injury Induced by Polygoni Multiflori Radix Praeparata [pubmed:27803670]
Pharmacological reports The Role of PON-1, GR, IL-18, and oxLDL in Depression With and Without Posttraumatic Stress Disorder [science:article]
International Journal of Molecular Sciences PEDF Inhibits the Activation of NLRP3 Inflammasome in Hypoxia Cardiomyocytes through PEDF Receptor/Phospholipase A2 [pubmed:27973457]
Cellular Physiology and Biochemistry MiR-155 is Involved in Renal Ischemia-Reperfusion Injury via Direct Targeting of FoxO3a andRegulating Renal Tubular Cell Pyroptosis. [pubmed:28006785]
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