粘蛋白5B(MUC5B)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Mucin 5 Subtype B (MUC5B)

MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5,Subtype B,Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin

  • 粘蛋白5B(MUC5B)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 粘蛋白5B(MUC5B)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对粘蛋白5B(MUC5B)具有高灵敏度和高特异性，经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板，4度孵育过夜或者37度孵育2小时；
2.洗板1次；
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时；
4.洗板1次。酶标板即可用于ELISA实验，后续使用方法与ELISA同。

储存

抗体，标准品和HRP标记的链霉亲和素在-20度保存，TMB需在4度保存，96孔板在室温保存。所有组分保质期为1年，试剂一旦开封，在4度保存时间为1个月。

配套试剂

相关产品

编号 适用物种：Mus musculus (Mouse，小鼠) 应用(仅供研究使用，不用于临床诊断！)
RPA684Mu02 粘蛋白5B(MUC5B)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA684Mu01 粘蛋白5B(MUC5B)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA684Mu01 粘蛋白5B(MUC5B)多克隆抗体 WB; IHC; ICC; IP.
PAA684Mu02 粘蛋白5B(MUC5B)多克隆抗体 WB; IHC; ICC; IP.
LAA684Mu81 粘蛋白5B(MUC5B)多克隆抗体(异硫氰酸荧光素标记) Applications: WB; IHC; ICC; IP.
LAA684Mu71 粘蛋白5B(MUC5B)多克隆抗体(生物素标记) WB; IHC; ICC.
SEA684Mu 粘蛋白5B(MUC5B)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA684Mu 粘蛋白5B(MUC5B)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.
KSA684Mu01 粘蛋白5B(MUC5B)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
The Journal of Biological Chemistry The Extracellular Signal-regulated Kinase Mitogen-activated Protein Kinase/Ribosomal S6 Protein Kinase 1 Cascade Phosphorylates cAMP Response Element-binding Protein to Induce MUC5B Gene Expression via D-Prostanoid Receptor Signaling [Jcb: 34199]
The Journal of Biological Chemistry The ERK MAPK/RSK1 cascade phosphorylates CREB to induce MUC5B gene expression via DP1 receptor signaling [Jcb: 247684]
Respiratory Researh Pyocyanin-induced mucin production is associated with redox modification of FOXA2 [PubMed: PMC3765780]
Respirology Secreted mucins and airway bacterial colonization in non-CF bronchiectasis [PubMed: 26172851]
American Thoracic Society International Conference Abstracts Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness [doi:10.1164]
Ann Am Thorac Soc Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization [doi:10.1513]
Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой [10.20538/1682-0363-2017-2-146-158]
Allergy Th2 cytokines orchestrate the secretion of MUC 5 AC and MUC 5B in IL‐5 positive chronic rhinosinusitis with nasal polyps [Pubmed:29802623]
European Journal of Pharmacology  Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling [Pubmed:29382534]
Japan Society of Immunology & Allergology in Otolaryngology インフルエンザ菌Phosphorylcholine の表出と中耳ムチンMUC5AC 及びMUC5B 産生への影響 [10.5648:jjiao.36.7]
Journal of Investigative Medicine The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease [Pubmed: 30573494]
Mucosal Immunology ASC and NLRP3 maintain innate immune homeostasis in the airway through an inflammasome-independent mechanism [Pubmed: 31278375]
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