调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法)

ELISA Kit DIY Materials for Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES)

CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5

  • 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法) 产品包装(模拟)
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

特异性

试剂盒中的抗体对调节激活正常T-细胞表达分泌因子(RANTES)具有高灵敏度和高特异性,经检测与其它相似物质无明显交叉反应。

用法

1.用100μL每孔的工作浓度的包被抗体包被96孔板,4度孵育过夜或者37度孵育2小时;
2.洗板1次;
3.用200μL每孔的工作浓度的封闭液,37度孵育1.5小时;
4.洗板1次。酶标板即可用于ELISA实验,后续使用方法与ELISA同。

储存

抗体,标准品和HRP标记的链霉亲和素在-20度保存,TMB需在4度保存,96孔板在室温保存。所有组分保质期为1年,试剂一旦开封,在4度保存时间为1个月。

配套试剂

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
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SCA116Hu 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
KSA116Hu01 调节激活正常T-细胞表达分泌因子(RANTES)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
J Thorac Dis.? Anti-endothelial cell antibodies in connective tissue diseases associated with pulmonary arterial hypertension [Pubmed:Pmc4015009]
Pharmacol Rep.? Comparison of chemokines (CCL-5 and SDF-1), chemokine receptors (CCR-5 and CXCR-4) and IL-6 levels in patients with different severities of depression [Pubmed:25150002]
Pharmacological Reports The MCP-1, CCL-5 and SDF-1 chemokines as pro-inflammatory markers in generalized anxiety disorder and personality disorders [Pubmed:25560580]
Pharmacological Reports Serum concentrations of chemokines (CCL-5 and CXCL-12), chemokine receptors (CCR-5 and CXCR-4), and IL-6 in patients with posttraumatic stress disorder and avoidant personality disorder [PubMed: 26481549]
Veterinary Parasitology Expression of adhesion molecules, chemokines and matrix metallo- proteinases (MMPs) in viable and degenerating stage of Taenia solium metacestode in swine neurocysticercosis [PubMed: 26412140]
Pharmacological Reports A study of chemokines, chemokine receptors and interleukin-6 in patients with panic disorder, personality disorders and their co-morbidity [Pubmed:27208878]
PLoS One.  Diacerein inhibits the pro-atherogenic & pro-inflammatory effects of IL-1 on human keratinocytes & endothelial cells. [pubmed:28323859]
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