膜联蛋白A5(ANXA5)活性蛋白

Active Annexin V (ANXA5)

ANX5; ENX2; PP4; PAP-I; CBP-I; Anchorin CII; Calphobindin I; Endonexin II; Lipocortin V; Placental anticoagulant protein 4; Thromboplastin inhibitor; Vascular anticoagulant-alpha

  • 膜联蛋白A5(ANXA5)活性蛋白 产品包装(模拟)
  • 膜联蛋白A5(ANXA5)活性蛋白 产品包装(模拟)
  • APA259Ra01.png Figure. SDS-PAGE
  • 膜联蛋白A5(ANXA5)活性蛋白 WB图
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Annexin V (ANXA5) is a multifunctional protein that is highly expressed on the apical surfaces of syncytiotrophoblasts, and plays an important role in haemostatic regulations, maintaining blood fluidity of the placenta. Lower ANXA5 levels have been observed in M2/ANXA5 haplotype carrying chorion. The association found between the maternal carriage of the M2/ANXA5 haplotype and an elevated risk of IUGR and/or PE supports the hypothesis that carrier status of this haplotype and the consequently reduced placental ANXA5 expression might be responsible, at least partially, for the onset of these gestational vascular complications. ANXA5 could be used as a biomarker for the early detection of PE and for the prediction of its severity. ANXA5 as an embryonic anticoagulant that appears deficient in contiguous specter of thrombophilia-related pregnancy complications culminating more frequently in miscarriage in a maternal M2 carrier background. Besides, Gamma Actin (ACTG) has been identified as an interactor of ANXA5, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat ANXA5 and recombinant human ACTG. Briefly, ANXA5 were diluted serially in PBS, with 0.01% BSA (pH7.4). Duplicate samples of 100 μl were then transferred to ACTG-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ANXA5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450 nm immediately. The binding activity of ANXA5 and ACTG was shown in Figure 1, and this effect was in a dose dependent manner, the EC50 was 0.78 ug/ml.

Annexin V is a calcium-dependent phospholipid binding protein that can be used to bind Phosphatidylserine (PS) during an early apoptosis event where the PS becomes exposed at the cell surface. Jurkat cells were treated with 10 uM camptothecin for 4h, 2*105 cells which were resuspended in binding buffer were stained with 5 ug recombinant rat Annexin V-FITC and 10 ul Propidium iodide (PI) for 20min in dark room temperature. The flow cytometry was used to detect the early apoptotic and late apoptotic of camptothecin-treated Jurkat cells (Figure 2), the combination of Annexin V-FITC and propidium iodide allows for the distinction between early apoptotic cells (Annexin V-FITC positive and propidium iodide negative), late apoptotic and/or necrotic cells (Annexin V-FITC and propidium iodide positive), and viable cells (unstained). Thus, the recombinant rat Annexin V-FITC can bind Phosphatidylserine (PS) at early apoptosis of Jurkat.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Rattus norvegicus (Rat,大鼠) 应用(仅供研究使用,不用于临床诊断!)
RPA259Ra01 膜联蛋白A5(ANXA5)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA259Ra01 膜联蛋白A5(ANXA5)活性蛋白 Cell culture; Activity Assays.
PAA259Ra01 膜联蛋白A5(ANXA5)多克隆抗体 WB; IHC; ICC; IP.
LAA259Ra81 膜联蛋白A5(ANXA5)多克隆抗体(异硫氰酸荧光素标记) WB; IHC; ICC; IF.
LAA259Ra71 膜联蛋白A5(ANXA5)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA259Ra21 膜联蛋白A5(ANXA5)单克隆抗体 WB; IHC; ICC; IP.
SEA259Ra 膜联蛋白A5(ANXA5)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA259Ra 膜联蛋白A5(ANXA5)等多因子检测试剂盒(流式荧光发光法) FLIA Assay for Antigen Detection.

参考文献

杂志 参考文献
Molecular Biology Markers of apoptotic dysfunctions in schizophrenia [Pubmed: 24466757]
Neuroscience and Behavioral Physiology Involvement of Anomalous Apoptosis in Impairments to Synaptic Plasticity in Post-Traumatic Stress Disorder [Springer: Source]
Inflammation and Cell Signaling Collectins, C3 complement protein, annexin V and C-reactive protein in acute ischemic stroke: interrelation and implication to upregulated apoptosis and inflammation [Smartscitech: Source]
Lung Annexin A5 Protein as a Potential Biomarker for the Diagnosis of Asthma [Pubmed: 30182154]
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