Active Apolipoprotein C3 (APOC3)
Apo-C3; APOCIII; APOC-III
- 物种Mus musculus (Mouse，小鼠) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 5mg (保证有货 万一缺货 免费重做)
- 价格 暂无报价 暂无报价 暂无报价 暂无报价 暂无报价
- Figure. Gene Sequencing (Extract)
- Figure. SDS-PAGE
- Figure. Western Blot
- 通过ISO 9001、ISO 13485质量体系认证
Figure. The binding activity of APOC3 with PCYOX1.
Apolipoprotein C3 (APOC3) also known as apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. An increase in apoC-III levels induces the development of hypertriglyceridemia. Some evidences suggest an intracellular role for Apo-CIII in promoting the assembly and secretion of triglyceride-rich VLDL particles from hepatic cells under lipid-rich conditions. Besides, Prenylcysteine Oxidase 1 (PCYOX1) has been identified as an interactor of APOC3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse APOC3 and recombinant mouse PCYOX1. Briefly, APOC3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to PCYOX1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APOC3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of APOC3 and PCYOX1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Mus musculus (Mouse，小鼠)||应用(仅供研究使用，不用于临床诊断！)|
|APB890Mu01||载脂蛋白C3(APOC3)活性蛋白||Cell culture; Activity Assays.|
|RPB890Mu01||载脂蛋白C3(APOC3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB890Mu02||载脂蛋白C3(APOC3)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAB890Mu02||载脂蛋白C3(APOC3)多克隆抗体||WB; IHC; ICC; IP.|
|PAB890Mu01||载脂蛋白C3(APOC3)多克隆抗体||WB; IHC; ICC; IP.|
|SEB890Mu||载脂蛋白C3(APOC3)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB890Mu01||载脂蛋白C3(APOC3)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
|Atherosclerosis||Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations [Pubmed:26978583]|
|The Journal of Lipid Research||Effects of angiopoietin-like protein 3 deficiency on postprandial lipid and lipoprotein metabolism [Pubmed:27040449]|
|Journal of Chromatography A||Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]|