载脂蛋白C3(APOC3)活性蛋白

Active Apolipoprotein C3 (APOC3)

Apo-C3; APOCIII; APOC-III

  • 载脂蛋白C3(APOC3)活性蛋白 产品包装(模拟)
  • 载脂蛋白C3(APOC3)活性蛋白 产品包装(模拟)
  • 载脂蛋白C3(APOC3)活性蛋白 Figure. Gene Sequencing (Extract)
  • APB890Mu01.png Figure. SDS-PAGE
  • 载脂蛋白C3(APOC3)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of APOC3 with PCYOX1.
Apolipoprotein C3 (APOC3) also known as apo-CIII is a component of very low density lipoprotein (VLDL). APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to inhibit hepatic uptake of triglyceride-rich particles. An increase in apoC-III levels induces the development of hypertriglyceridemia. Some evidences suggest an intracellular role for Apo-CIII in promoting the assembly and secretion of triglyceride-rich VLDL particles from hepatic cells under lipid-rich conditions. Besides, Prenylcysteine Oxidase 1 (PCYOX1) has been identified as an interactor of APOC3, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse APOC3 and recombinant mouse PCYOX1. Briefly, APOC3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to PCYOX1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APOC3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of APOC3 and PCYOX1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Mus musculus (Mouse,小鼠) 应用(仅供研究使用,不用于临床诊断!)
APB890Mu01 载脂蛋白C3(APOC3)活性蛋白 Cell culture; Activity Assays.
RPB890Mu01 载脂蛋白C3(APOC3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB890Mu02 载脂蛋白C3(APOC3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB890Mu02 载脂蛋白C3(APOC3)多克隆抗体 WB; IHC; ICC; IP.
PAB890Mu01 载脂蛋白C3(APOC3)多克隆抗体 WB; IHC; ICC; IP.
SEB890Mu 载脂蛋白C3(APOC3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
KSB890Mu01 载脂蛋白C3(APOC3)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
Atherosclerosis Up-reCavia (Guinea pig )lation of Hnf1α gene expression in the liver of rats with experimentally induced chronic renal failure – A possible link between circulating PCSK9 and triacylglycerol concentrations [Pubmed:26978583]
The Journal of Lipid Research Effects of angiopoietin-like protein 3 deficiency on postprandial lipid and lipoprotein metabolism [Pubmed:27040449]
Journal of Chromatography A Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858]
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