B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白

Active B-Lymphocyte Activation Antigen B7-1 (LAB7-1)

CD80; CD28LG; CD28LG1; BB1; B7-1 Antigen; T-lymphocyte activation antigen CD80; CTLA-4 counter-receptor B7.1

  • B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白 产品包装(模拟)
  • B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白 产品包装(模拟)
  • APB386Hu61.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

B-Lymphocyte Activation Antigen B7-1 (CD80) is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. CD80 can combine with CD86, thus, a binding ELISA assay was conducted to detect the association of recombinant human CD80 with recombinant human CD86. Briefly, biotin-linked recombinant human CD80 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to CD86-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human CD80 and recombinant human CD86 was shown in Figure 1, the EC50 for this effect is 0.412 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
EPB386Hu61 B-淋巴细胞激活抗原B7-1(LAB7-1)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB386Hu61 B-淋巴细胞激活抗原B7-1(LAB7-1)活性蛋白 Cell culture; Activity Assays.
PAB386Hu06 B-淋巴细胞激活抗原B7-1(LAB7-1)多克隆抗体 WB; IHC; ICC; IP.
PAB386Hu01 B-淋巴细胞激活抗原B7-1(LAB7-1)多克隆抗体 WB; IHC; ICC; IP.
SEB386Hu B-淋巴细胞激活抗原B7-1(LAB7-1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB386Hu B-淋巴细胞激活抗原B7-1(LAB7-1)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
BioMed Research International Predictability of Urinary CD80 in the Relapse of Primary Nephrotic Syndrome [pubmed:28951877]
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