肝细胞生长因子受体(MET)活性蛋白

Active C-Met (MET)

HGFR; RCCP2; Hepatocyte Growth Factor Receptor; Met Proto-Oncogene; Mesenchymal-Epithelial Transition Factor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; Tyrosine-protein kinase M

  • 肝细胞生长因子受体(MET)活性蛋白 产品包装(模拟)
  • 肝细胞生长因子受体(MET)活性蛋白 产品包装(模拟)
  • APC186Hu01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

c-Met is a receptor tyrosine kinase belonging to the MET (MNNG HOS transforming gene) family, and is expressed on the surfaces of various cells. Hepatocyte growth factor (HGF) is the ligand for this receptor. The binding of HGF to c-Met initiates a series of intracellular signals that mediate embryogenesis and wound healing in normal cells. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MET and recombinant human HGF. Briefly, MET was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to HGF-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-MET pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 µL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human MET and recombinant human HGF was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
RPC186Hu01 肝细胞生长因子受体(MET)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APC186Hu01 肝细胞生长因子受体(MET)活性蛋白 Cell culture; Activity Assays.
RPC186Hu02 肝细胞生长因子受体(MET)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC186Hu01 肝细胞生长因子受体(MET)多克隆抗体 WB; IHC
MAC186Hu22 肝细胞生长因子受体(MET)单克隆抗体 WB; IHC; ICC; IP.
SEC186Hu 肝细胞生长因子受体(MET)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC186Hu 肝细胞生长因子受体(MET)等多因子检测试剂盒(流式荧光发光法) FLIA Kit for Antigen Detection.

参考文献

杂志 参考文献
scientific reports New candidate blood biomarkers potentially associated with white matter hyperintensities progression [34253757]
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