钙蛋白酶3(CAPN3)活性蛋白

Active Calpain 3 (CAPN3)

CANPL3; p94; LGMD2; LGMD2A; nCL1; Calcium-activated neutral proteinase 3; Muscle-specific calcium-activated neutral protease 3; New calpain 1

  • 钙蛋白酶3(CAPN3)活性蛋白 产品包装(模拟)
  • 钙蛋白酶3(CAPN3)活性蛋白 产品包装(模拟)
  • APC960Hu01.png Figure. SDS-PAGE
  • 钙蛋白酶3(CAPN3)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of CAPN3 with TTN.
Calpain 3 is a calcium-dependent cysteine protease mainly expressed in skeletal muscle. In humans, calpain 3 is encoded by the CAPN3 gene. This gene encodes a muscle-specific member of the calpain large subunit family that specifically binds to titin. Mutations in this gene are associated with limb-girdle muscular dystrophies type 2A. Alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms and some variants are ubiquitously expressed. Besides, Titin (TTN) has been identified as an interactor of CAPN3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CAPN3 and recombinant human TTN. Briefly, CAPN3 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to TTN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CAPN3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CAPN3 and TTN was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APC960Hu01 钙蛋白酶3(CAPN3)活性蛋白 Cell culture; Activity Assays.
RPC960Hu01 钙蛋白酶3(CAPN3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAC960Hu01 钙蛋白酶3(CAPN3)多克隆抗体 WB; IHC; ICC; IP.
MAC960Hu22 钙蛋白酶3(CAPN3)单克隆抗体 WB; IHC; ICC; IP.
SEC960Hu 钙蛋白酶3(CAPN3)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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