羧酸酯酶3(CES3)活性蛋白

Active Carboxylesterase 3 (CES3)

Br3; ES31; Brain carboxylesterase 3; Esterase 31; Liver carboxylesterase 31 homolog

  • 羧酸酯酶3(CES3)活性蛋白 产品包装(模拟)
  • 羧酸酯酶3(CES3)活性蛋白 产品包装(模拟)
  • APF283Ra01.jpg Figure. SDS-PAGE
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Carboxylesterase 3 (CES3) also known as Liver carboxylesterase 3 is a serine esterase and a member of a large family of carboxylesterases. The protein Involved in the detoxification of xenobiotics and in the activation of ester and amide prodrugs. Hydrolyzes aromatic and aliphatic esters, but has no catalytic activity toward amides or a fatty acyl-CoA ester. Hydrolyzes the methyl ester group of cocaine to form benzoylecgonine. Thus, the recombinant Rat CES3 activity was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 50 mM Tris, pH 7.5( Assay Buffer), initiated by addition 50 μL of various concentrations of CES3 (dilute by Assay Buffer) to 50 µL of 2 mM Substrate 4-NPA(100 mM stock in Acetone, dilute by deionized water). Incubated at 37℃ for 10min, then read at a wavelength of 400 nm.

One unit of enzyme activity is defined as the 1μg of enzyme required to convert 1pmol of 4-Nitrophenyl acetate to 4-Nitrophenol in 1min at 37°C. The specific activity of recombinant Rat CES3 is 1200 pmol/min/µg.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Rattus norvegicus (Rat，大鼠) 应用(仅供研究使用，不用于临床诊断！)
APF283Ra01 羧酸酯酶3(CES3)活性蛋白 Cell culture; Activity Assays.
RPF283Ra01 羧酸酯酶3(CES3)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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