组织蛋白酶V(CTSV)活性蛋白

Active Cathepsin V (CTSV)

CTS-L2; CTSL2; CATL2; CTSU; CTS-V; Cathepsin L2

  • 组织蛋白酶V(CTSV)活性蛋白 产品包装(模拟)
  • 组织蛋白酶V(CTSV)活性蛋白 产品包装(模拟)
  • APC970Hu01.jpg SDS-PAGE图
  • 组织蛋白酶V(CTSV)活性蛋白 Figure. Western Blot; Sample: Recombinant CTSV, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Cathepsin V (CTSV) is a lysosomal cysteine proteinase which belongs to peptidase C1 family. It is expressed in normal human thymus, testis and corneal epithelium. Cathepsin V plays an important role in corneal physiology and mediates degradation of invariant chain in human thymus. Besides, Retinoblastoma Protein 1 (RB1) has been identified as an interactor of CTSV, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CTSV and recombinant human RB1. Briefly, CTSV were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RB1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-CTSV pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of CTSV and RB1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

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编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
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