Active Dickkopf Related Protein 1 (DKK1)
SK; Dickkopf Homolog 1(Xenopus Laevis)
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份磷酸盐缓冲液（pH7.4，含有 0.01% SKL, 1mM DTT, 5% Trehalose和Proclin300.）
- 纯度> 95%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 1g (保证有货 万一缺货 免费重做)
- 价格 ¥ 1728 ¥ 4320 ¥ 8640 ¥ 25920 ¥ 648000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant DKK1, Human.
- 通过ISO 9001、ISO 13485质量体系认证
Dickkopf-related protein 1 (DKK1) is a protein which is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the LRP6 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. Besides, Low Density Lipoprotein Receptor Related Protein 6 (LRP6) has been identified as an interactor of DKK1 thus a binding ELISA assay was conducted to detect the interaction of recombinant human DKK1 and recombinant human LRP6. Briefly, DKK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LRP6-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DKK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DKK1 and LRP6 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APA741Hu01||Dickkopf相关蛋白1(DKK1)活性蛋白||Cell culture; Activity Assays.|
|RPA741Hu01||Dickkopf相关蛋白1(DKK1)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAA741Hu01||Dickkopf相关蛋白1(DKK1)多克隆抗体||WB; IHC; ICC; IP.|
|MAA741Hu22||Dickkopf相关蛋白1(DKK1)单克隆抗体||WB; IHC; ICC; IP.|
|SEA741Hu||Dickkopf相关蛋白1(DKK1)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|