Dickkopf相关蛋白1(DKK1)活性蛋白

Active Dickkopf Related Protein 1 (DKK1)

SK; Dickkopf Homolog 1(Xenopus Laevis)

  • Dickkopf相关蛋白1(DKK1)活性蛋白 产品包装(模拟)
  • Dickkopf相关蛋白1(DKK1)活性蛋白 产品包装(模拟)
  • Dickkopf相关蛋白1(DKK1)活性蛋白 Gene sequencing
  • APA741Hu01.jpg SDS-PAGE图
  • Dickkopf相关蛋白1(DKK1)活性蛋白 Figure. Western Blot; Sample: Recombinant DKK1, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Dickkopf-related protein 1 (DKK1) is a protein which is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the LRP6 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. Besides, Low Density Lipoprotein Receptor Related Protein 6 (LRP6) has been identified as an interactor of DKK1 thus a binding ELISA assay was conducted to detect the interaction of recombinant human DKK1 and recombinant human LRP6. Briefly, DKK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LRP6-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DKK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DKK1 and LRP6 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

赠品

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
APA741Hu01 Dickkopf相关蛋白1(DKK1)活性蛋白 Cell culture; Activity Assays.
RPA741Hu01 Dickkopf相关蛋白1(DKK1)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA741Hu01 Dickkopf相关蛋白1(DKK1)多克隆抗体 WB; IHC; ICC; IP.
MAA741Hu22 Dickkopf相关蛋白1(DKK1)单克隆抗体 WB; IHC; ICC; IP.
SEA741Hu Dickkopf相关蛋白1(DKK1)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.
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