中性粒细胞弹性蛋白酶(ELA2)活性蛋白

Active Elastase 2, Neutrophil (ELA2)

ELANE; HLE; HNE; NE; PMN-E; Neutrophil Elastase; Medullasin; Polymorphonuclear Leukocyte Elastase; Bone Marrow Serine Protease; Human Leukocyte Elastase; PMN Elastase

  • 中性粒细胞弹性蛋白酶(ELA2)活性蛋白 产品包装(模拟)
  • 中性粒细胞弹性蛋白酶(ELA2)活性蛋白 产品包装(模拟)
  • APA181Hu01.png Figure. SDS-PAGE
  • 中性粒细胞弹性蛋白酶(ELA2)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure 1. The binding activity of ELA2 with COL17.
Elastase2 (ELA2), elastase 2 is a serine proteinase in the same family as chymotrypsin and has broad substrate specificity. Secreted by neutrophils and macrophages during inflammation, it destroys bacteria and host tissue. The neutrophil form of elastase is 218 amino acids long, with two asparagine-linked carbohydrate chains (see glycosylation). Neutrophil elastase may play a role in degenerative and inflammatory diseases by its proteolysis of collagen-IV and elastin of the extracellular matrix. This protein degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Besides, Collagen Type XVII (COL17) has been identified as an interactor of ELA2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human COL17 and recombinant human ELA2. Briefly, ELA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to COL17-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-ELA2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of ELA2 and COL17 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Homo sapiens (Human,人) 应用(仅供研究使用,不用于临床诊断!)
RPA181Hu01 中性粒细胞弹性蛋白酶(ELA2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA181Hu01 中性粒细胞弹性蛋白酶(ELA2)活性蛋白 Cell culture; Activity Assays.
PAA181Hu01 中性粒细胞弹性蛋白酶(ELA2)多克隆抗体 WB; IHC; ICC; IP.
MAA181Hu22 中性粒细胞弹性蛋白酶(ELA2)单克隆抗体 WB; IHC; ICC; IP.
SEA181Hu 中性粒细胞弹性蛋白酶(ELA2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
Mediators of Inflammation Long-Term Leukocyte Filtration Should Be Avoided during Extracorporeal Circulation [Hindawi: 612848]
Journal of Photochemistry and Photobiology B: Biology A dietary supplement improves facial photoaging and skin sebum, hydration and tonicity modulating serum fibronectin, neutrophil elastase 2, hyaluronic acid and carbonylated proteins [Pubmed:25732262]
International Journal of Molecular Medicine The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats [pubmed:27430552]
European Journal of Trauma and Emergency Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in acanine burn model. [pubmed:28070608]
Clin Exp Pharmacol Physiol. Inhibition of ALDH2 protects PC12 cells against formaldehyde-induced cytotoxicity: involving the protection of hydrogen sulphide. [pubmed:28251688]
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