表皮生长因子(EGF)活性蛋白

Active Epidermal Growth Factor (EGF)

URG; Beta-Urogastrone; Pro-epidermal growth factor

  • 表皮生长因子(EGF)活性蛋白 产品包装(模拟)
  • 表皮生长因子(EGF)活性蛋白 产品包装(模拟)
  • 表皮生长因子(EGF)活性蛋白 Figure. Gene Sequencing (Extract)
  • APA560Hu02.png Figure. SDS-PAGE
  • 表皮生长因子(EGF)活性蛋白 Figure. Western Blot; Sample: Recombinant EGF, Human.
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .
Cell proliferation of 3T3 cells after incubation with EGF for 72h observed by inverted microscope was shown in Figure 1.

The dose-effect curve of EGF was shown in Figure 2. It was obvious that EGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 0.9848 to 2.958ng/mL.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月，-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定，具体方法如下：在37°C孵育48小时，没有显著的降解或者沉淀产生。保质期内，在适当的条件下存储，损失率低于5%。

相关产品

编号 适用物种：Homo sapiens (Human，人) 应用(仅供研究使用，不用于临床诊断！)
APA560Hu02 表皮生长因子(EGF)活性蛋白 Cell culture; Activity Assays.
RPA560Hu03 表皮生长因子(EGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA560Hu08 表皮生长因子(EGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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RPA560Hu06 表皮生长因子(EGF)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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PAA560Hu01 表皮生长因子(EGF)多克隆抗体 WB; IHC; ICC; IP.
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LAA560Hu71 表皮生长因子(EGF)多克隆抗体(生物素标记) WB; IHC; ICC.
MAA560Hu22 表皮生长因子(EGF)单克隆抗体 WB; IHC
MAA560Hu24 表皮生长因子(EGF)单克隆抗体 WB; IHC; ICC; IP.
MAA560Hu23 表皮生长因子(EGF)单克隆抗体 WB; IHC; ICC; IP.
MAA560Hu25 表皮生长因子(EGF)单克隆抗体 WB; IHC; ICC; IP.
MAA560Hu27 表皮生长因子(EGF)单克隆抗体 WB; IHC; ICC; IP.
MAA560Hu21 表皮生长因子(EGF)单克隆抗体 WB; IHC; ICC; IP.
HEA560Hu 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
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WEA560Hu 表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法,宽范围) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA560Hu 表皮生长因子(EGF)检测试剂盒(化学发光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
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PSA560Hu01 表皮生长因子(EGF)抗体对 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSA560Hu01 表皮生长因子(EGF)检测试剂盒DIY材料(酶联免疫吸附试验法) Main materials for "Do It (ELISA Kit) Yourself".

参考文献

杂志 参考文献
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Biomark Insights Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]
Plos one The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171]
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Eur Rev Med Pharmacol Sci.? Association of colon adenomas and skin tags: coincidence or coexistence? [Pubmed:24763889]
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ACS Applied Materials & Interfaces Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. [pubmed:27709887]
Canadian Journal of Physiology and Pharmacology The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]
Journal of Controlled Release A DPP-IV-resistant glucagon-like peptide-2 dimer with enhanced activity against radiation-induced intestinal injury [pubmed:28522195]
Scientific Reports Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets [pubmed:28811665]
International Journal of Nanomedicine Micro-/nano-topography of selective laser melting titanium enhances adhesion and proliferation and regulates adhesion-related gene expressions of human … [Pubmed: 30233172]
Neoplasia Patient-derived xenograft (PDX) models of colorectal carcinoma (CRC) as a platform for chemosensitivity and biomarker analysis in personalized medicine [Pubmed: 33212364]
Aesthet Surg J Adipose-Derived Stem Cells Improve the Aging Skin of Nude Mice by Promoting Angiogenesis and Reducing Local Tissue Water [33428732]
Cell Death & Disease Sodium/glucose cotransporter 1-dependent metabolic alterations induce tamoxifen resistance in breast cancer by promoting macrophage M2 polarization [34006822]
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Biomaterials Advances Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats []
Authorea Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy []
Biointerface Research in Applied Chemistry Beetroot Improves the Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells in Preventing Cisplatin Induced Nephrotoxicity in Male Rats []
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