Active Epidermal Growth Factor (EGF)
URG; Beta-Urogastrone; Pro-epidermal growth factor
- 物种Homo sapiens (Human，人) 相同的名称，不同的物种。
- 缓冲液成份20mM Tris, 150mM NaCl缓冲液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 纯度> 90%
- 应用Cell culture; Activity Assays.
- 下载 英文说明书 中文说明书
- 规格 10µg50µg 200µg 1mg 1g (保证有货 万一缺货 免费重做)
- 价格 ¥ 768 ¥ 1920 ¥ 3840 ¥ 11520 ¥ 288000
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant EGF, Human.
- 通过ISO 9001、ISO 13485质量体系认证
Epidermal growth factor (EGF) is a growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. To test the effect of EGF on cell proliferation of 3T3 fibroblasts, 3T3 cells were seeded into triplicate wells of 96-well plates at a density of 2, 000cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of EGF. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37°C .
Cell proliferation of 3T3 cells after incubation with EGF for 72h observed by inverted microscope was shown in Figure 1.
The dose-effect curve of EGF was shown in Figure 2. It was obvious that EGF significantly promoted cell proliferation of 3T3 cells. The ED50 for this effect is typically 0.9848 to 2.958ng/mL.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
|编号||适用物种：Homo sapiens (Human，人)||应用(仅供研究使用，不用于临床诊断！)|
|APA560Hu02||表皮生长因子(EGF)活性蛋白||Cell culture; Activity Assays.|
|RPA560Hu02||表皮生长因子(EGF)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPA560Hu01||表皮生长因子(EGF)重组蛋白||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA560Hu01||表皮生长因子(EGF)活性蛋白||Cell culture; Activity Assays.|
|PAA560Hu01||表皮生长因子(EGF)多克隆抗体||WB; IHC; ICC; IP.|
|PAA560Hu02||表皮生长因子(EGF)多克隆抗体||WB; IHC; ICC; IP.|
|LAA560Hu71||表皮生长因子(EGF)多克隆抗体(生物素标记)||WB; IHC; ICC.|
|MAA560Hu22||表皮生长因子(EGF)单克隆抗体||WB; IHC; ICC; IP.|
|MAA560Hu21||表皮生长因子(EGF)单克隆抗体||WB; IHC; ICC; IP.|
|SEA560Hu||表皮生长因子(EGF)检测试剂盒(酶联免疫吸附试验法)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA560Hu||表皮生长因子(EGF)检测试剂盒(化学发光免疫分析法)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA560Hu01||表皮生长因子(EGF)检测试剂盒DIY材料(酶联免疫吸附试验法)||Main materials for "Do It (ELISA Kit) Yourself".|
|Journal of Pediatric Surgery||The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940]|
|Biomark Insights||Decreased Epidermal Growth Factor (EGF) Associated with HMGB1 and Increased Hyperactivity in Children with Autism [PubMed: PMC3623607]|
|Plos one||The Probiotic Mixture VSL#3 Accelerates Gastric Ulcer Healing by Stimulating Vascular Endothelial Growth Factor [PubMed: PMC3590171]|
|Diabetes.||Allogeneic transplantation of an adipose-derived stem cell (ASC) sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity [Pubmed:25795216]|
|Eur Rev Med Pharmacol Sci.?||Association of colon adenomas and skin tags: coincidence or coexistence? [Pubmed:24763889]|
|Int J Clin Exp Med.||The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972]|
|ACS Applied Materials & Interfaces||Polydopamine nanoparticles modulating stimuli-responsive PNIPAM hydrogels with cell/tissue adhesiveness. [pubmed:27709887]|
|Canadian Journal of Physiology and Pharmacology||The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats [pubmed:28177680]|