碱性成纤维细胞生长因子(FGF2)活性蛋白

Active Fibroblast Growth Factor 2, Basic (FGF2)

B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2

  • 碱性成纤维细胞生长因子(FGF2)活性蛋白 产品包装(模拟)
  • 碱性成纤维细胞生长因子(FGF2)活性蛋白 产品包装(模拟)
  • APA551Bo01.jpg Figure. SDS-PAGE
  • 碱性成纤维细胞生长因子(FGF2)活性蛋白 Figure. Western Blot
  • Certificate 通过ISO 9001、ISO 13485质量体系认证

活性实验

Figure. The binding activity of FGF2 with CASP1.
Basic fibroblast growth factor (FGF2), also known as bFGF, FGF-β is a member of a large family of structurally related heparin-binding proteins (the FGFs) involved in the regulation of cell proliferation, growth and differentiation. It involved in many biological processes including angiogenesis, embryonic development and wound healing. Additionally, FGF2 is a critical component of human embryonic stem cell culture medium. Besides, Caspase 1 (CASP1) has been identified as an interactor of FGF2, thus a binding ELISA assay was conducted to detect the interaction of recombinant bovine FGF2 and recombinant bovine CASP1. Briefly, FGF2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CASP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FGF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of FGF2 and CASP1 was shown in Figure 1, and this effect was in a dose dependent manner.

用法

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

储存

避免反复冻融。2-8°C不超过一个月,-80°C不超过12个月。

稳定性

热稳定性以损失率显示。损失率是由加速降解试验决定,具体方法如下:在37°C孵育48小时,没有显著的降解或者沉淀产生。保质期内,在适当的条件下存储,损失率低于5%。

相关产品

编号 适用物种:Bos taurus; Bovine (Cattle,牛) 应用(仅供研究使用,不用于临床诊断!)
RPA551Bo01 碱性成纤维细胞生长因子(FGF2)重组蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APA551Bo01 碱性成纤维细胞生长因子(FGF2)活性蛋白 Cell culture; Activity Assays.
PAA551Bo01 碱性成纤维细胞生长因子(FGF2)多克隆抗体 WB; IHC; ICC; IP.
MAA551Bo21 碱性成纤维细胞生长因子(FGF2)单克隆抗体 WB; IHC; ICC; IP.
CEA551Bo 碱性成纤维细胞生长因子(FGF2)检测试剂盒(酶联免疫吸附试验法) Enzyme-linked immunosorbent assay for Antigen Detection.

参考文献

杂志 参考文献
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Science Alert Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source]
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